Advanced SearchSearch Tips
Genetic Mapping of QTLs that Control Grain Characteristics in Rice (Oryza sativa L.)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 25, Issue 8,  2015, pp.925-931
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.8.925
 Title & Authors
Genetic Mapping of QTLs that Control Grain Characteristics in Rice (Oryza sativa L.)
Wacera, Home Regina; Safitri, Fika Ayu; Lee, Hyun-Suk; Yun, Byung-Wook; Kim, Kyung-Min;
  PDF(new window)
We performed a molecular marker-based analysis of quantitative trait loci (QTLs) for traits that determine the quality of the appearance of grains, using 120 doubled-haploid (DH) lines developed by another culture from the F1 cross between ‘Cheongcheong’ (Oryza sativa L. ssp. Indica) and ‘Nagdong’ (Oryza sativa L. ssp. Japonica). The traits studied included length, width, and thickness of the grains, as well as length-to-width ratio and 1,000-grain weight. The objective of this study was to determine the genetic control of these traits in order to formulate a strategy for improving the appearance of this hybrid. Within the DH population, five traits exhibited wide variation, with mean values occurring within the range of the two parents. Three QTLs were identified for grain length on chromosomes 2, 5, and 7. Three QTLs were mapped for grain width on chromosome 2: qGW2-1, qGW2-2, and qGW2-3. Six chromosomes were identified for the grain length-to-width ratio; four of these were on chromosome 2, whereas the other two were on chromosomes 7 and 12. One QTL influencing 1,000-grain weight was identified and located on chromosome 8. The results presented in the present study should facilitate rice-breeding, especially for improved hybrid-rice quality.
Doubled haploid;grain quality;QTL;rice;
 Cited by
He, P., Li, S. G., Qian, Q., Ma, Y. Q., Li, J. Z., Wang, W. M., Chen, Y. and Zhu, L. H. 1999. Genetic analysis of rice grain quality. Theor. Appl. Genet. 98, 502-508. crossref(new window)

Huang, R., Jiang, L., Zheng, J., Wang, T., Wang, T., Huang, Y. and Hong, Z. 2012. Genetic bases of rice grain shape: so many genes, so little known. Trends Plant Sci. 18, 218-226.

Jiang, G. H., Hong, X. Y., Xu, C. G., Li, X. H. and He, Y. Q. 2005. Identification of Quantitative Trait Loci for grain appearance and milling quality using a double-haploid rice population. J. Integr. Plant Biol. 47, 1391-1403. crossref(new window)

Juliano, B. O. and Villareal, C. P. 1993. Grain quality evaluation of World rice. International Rice Research Institute.

Li, Z., Pinson, S. R. M., Park, W. D., Paterson, A. H. and Stansel, J. W. 1997. Epistasis for three grain yield components in rice (Oryza sativa L.). Genetics 145, 453-465.

Lin, H. X., Qian, H. R., Zhuang, J. Y., Lu, J., Min, S. K., Xiong, Z. M., Huang, N. and Zheng, K. L. 1996. RFLP mapping of QTLs for yield and related characters in rice (Oryza sativa L.). Theor. Appl. Genet. 92, 920-927. crossref(new window)

Moncada, P., Martínez, C. P., Borrero, J., Chatel, M., Gauch Jr, H., Guimaraes, E., Tohme, J. and McCouch, S. R. 2001. Quantitative trait loci for yield and yield components in an O. sativa x O. rufipogon BC2F2 population evaluated in an upland environment. Theor. Appl. Genet. 102, 41-52. crossref(new window)

Qin, Y., Kim, S. M. and Sohn, J. K. 2008. Detection of main-effect QTLs, epistatic QTLs and QE interactions for grain appearance of brown rice (Oryza sativa L.). J. Crop Sci. Biotech. 11, 151-156.

Rabiei, B., Valizadeh, M., Ghareyazie, B., Moghaddam, M. and Ali, A. J. 2004. Identification of QTLs for rice grain size and shape of Iranian cultivars using SSR markers. Euphytica 137, 325-332. crossref(new window)

Redoña, E. D. and Mackill, D. J. 1998. Quantitative trait locus analysis for rice panicle and grain characteristics. Theor. Appl. Genet. 96, 957-963. crossref(new window)

Tan, Y. F., Xing, Y. Z., Li, J. X., Yu, S. B., Xu, C. G. and Zhang, Q. 2000. Genetic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid. Theor. Appl. Genet. 101, 823-829. crossref(new window)

Temnykh, S., Declerck, G., Luashova, A., Lipovich, L., Cartinhour, S. and MsCouch, S. 2011. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome Res. 11, 1441-1452.

Temnykh, S., Park, W. D., Ayres, N., Cartinhour, S., Hauck, N., Lipovich, L., Cho, Y. G., Ishii, T. and McCouch, S. R. 2000. Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor. Appl. Genet. 100, 697-712. crossref(new window)

Xiao, J., Li, J., Yuan, L. and Tanksley, S. D. 1996. Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific rice cross. Theor. Appl. Genet. 92, 230-244. crossref(new window)

Yan, B., Liu, R., Li, Y., Wang,Y., Gao, G., Zhang, Q., Liu, X., Jiang, G. and He, Y. 2014. QTL analysis on rice grain appaearance quality, as exemplifying the typical events of transgenic or backcrossing breeding. Breed. Sci. 64, 231-239. crossref(new window)

Yan, C. J., Liang, G. H., Chen, F., Li, X., Tang, S. Z., Yi, C. D., Tian, S., Lu, J. F. and Gu, M. H. 2003. Mapping quantitative trait loci associated with rice grain shape based on an indica/japonica backcross population. J. Genet. Genomics 30, 711-716.

Yano, M. and Sasaki, T. 1997. Genetic and molecular dissection of quantitative traits in rice. Plant Mol. Biol. 35, 145-153. crossref(new window)