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α-Asarone Modulates Activity of Matrix Metalloproteinase as well as Antioxidant Activity
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 9,  2015, pp.1000-1006
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.9.1000
 Title & Authors
α-Asarone Modulates Activity of Matrix Metalloproteinase as well as Antioxidant Activity
Park, Hye-Jung; Kim, Moon-Moo;
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α-Asarone is the main component of Acorus gramineus, which is a widely used oriental traditional medicine. A. gramineus is known to have a variety of medicinal effects, such as anti-gastric ulcer, antiallergy and antioxidant activity. It is also known to inhibit the release of histamine. However, the mechanism of its action remains unclear in humans. In this study, the effects of α-asarone on matrix metalloproteinase (MMP) and its antioxidant effect in a cell-free system were examined in HT1080 cells. In an MTT assay, the effect of α-asarone on cell viability showed no cytotoxicity below 16 μM. In an antioxidant assay, α-asarone increased reducing power in a dose-dependent manner but not the scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. In addition, α-asarone exhibited the protective effect against DNA oxidation induced by hydroxyl radicals produced by the Fenton reaction. Furthermore, in a gelatin disk assay, α-asarone enhanced collagenase activity. It also increased the activities of MMP-2 and MMP-9 stimulated by phorbol 12-myristate 13-acetate (PMA) in a gelatin zymography. On the other hand, the activity of MMP-9 stimulated by phenazine methosulfate (PMS) but not that of MMP-2 was increased in the presence of α-asarone. These findings suggest that α-asarone could be a candidate for the prevention and treatment of pathological diseases related to oxidative stress and MMPs.
α-Asarone;Acorus gramineus;antioxidant;collagenase;matrix metalloproteinase;
 Cited by
Apel, K. and Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55, 373-399. crossref(new window)

Bergers, G., Brekken, R., McMahon, G., Vu, T. H., Itoh, T., Tamaki, K., Tanzawa, K., Thorpe, P., Itohara, S., Werb, Z. and Hanahan, D. 2000. Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis. Nat. Cell Biol. 2, 737-744. crossref(new window)

Cho, J., Kim, Y. H., Kong, J. Y., Yang, C. H. and Park, C. G. 2002. Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus. Life Sci. 71, 591-599. crossref(new window)

Coussens, L. M., Fingleton, B. and Matrisian, L. M. 2002. Matrix metalloproteinase inhibitors and cancer-trials and tribulations. Science 295, 2387-2392. crossref(new window)

Hansen, M. B., Nielsen, S. E. and Berg, K. 1989. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods 119, 203-210. crossref(new window)

Imai, J., Ide, N., Nagae, S., Moriguchi, T., Matsuura, H. and Itakura, Y. 1994. Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Medica 60, 417-420. crossref(new window)

Jain, N., Jain, R., Jain, A., Jain, D. K. and Chandel, H. S. 2010. Evaluation of wound-healing activity of Acorus calamus Linn. Nat. Prod. Res. 24, 534-541. crossref(new window)

Kim, M. M., Ta, Q. V., Mendis, E., Rajapakse, N., Jung, W. K., Byun, H. G., Jeon, Y. J. and Kim, S. K. 2006. Phlorotannins in Ecklonia cava extract inhibit matrix metalloproteinase activity. Life Sci. 79, 1436-1443. crossref(new window)

Lee, K. W. and Lee, H. J. 2006. Biphasic effects of dietary antioxidants on oxidative stress-mediated carcinogenesis. Mechsm Age. Dev. 127, 424-431. crossref(new window)

Lee, S. J., Seo, K. W., Yun, M. R., Bae, S. S., Lee, W. S., Hong, K. W. and Kim, C. D. 2008. 4-Hydroxynonenal enhances MMP-2 production in vascular smooth muscle cells via mitochondrial ROS-mediated activation of the Akt/NF kappaBsignaling pathways. Free Radic. Biol. Med. 45, 1487-1492. crossref(new window)

Limón, I. D., Mendieta, L., Díaz, A., Chamorro, G., Espinosa, B., Zenteno, E. and Guevara, J. 2009. Neuroprotective effect of alpha-asarone on spatial memory and nitric oxide levels in rats injected with amyloid-β (25-35). Neurosci. Lett. 453, 98-103. crossref(new window)

Manikandan, S. and Devi, R. S. 2005. Antioxidant property of alphaasarone against noise-stress-induced changes in different regions of rat brain. Pharmacol. Res. 52, 467-474. crossref(new window)

Martin, P. 1997. Wound healing--aiming for perfect skin regeneration. Science 276, 75-81. crossref(new window)

Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7, 405-410. crossref(new window)

Neufeld, G., Cohen, T., Gengrinovitch, S. and Poltorak, Z. 1999. Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 13, 9-22.

Oyaizu, M. 1986. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44, 307-315. crossref(new window)

Pages, N., Maurois, P., Delplanque, B., Bac, P., Stables, J. P., Tamariz, J., Chamorro, G. and Vamecq, J. 2010. Activities of α-asarone in various animal seizure models and in biochemical assays might be essentially accounted for by antioxidant properties. Neurosci. Res. 68, 337-344. crossref(new window)

Park, H. J., Lee, S. J. and Kim, M. M. 2015. Effect of α-asarone on angiogenesis and matrix metalloproteinase. Environ. Toxico.l Pharmacol. 39, 1107-1114. crossref(new window)

Rodríguez-Páez, L., Juárez-Sanchez, M., Antúnez-Solís, J., Baeza, I. and Wong, C. 2003. α-Asarone inhibits HMG-CoA reductase, lowers serum LDL-cholesterol levels and reduces biliary CSI in hypercholesterolemic rats. Phytomedicine 10, 397-404. crossref(new window)

Salo, T., Mäkelä, M., Kylmäniemi, M., Autio-Harmainen, H. and Larjava, H. 1994. Expression of matrix metalloproteinase-2 and-9 during early human wound healing. Lab. Invest. 70, 176-182.

Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning: a laboratory manual: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1977.

Shin, J. W., Cheong, Y. J., Koo, Y. M., Kim, S., Noh, C. K., Son, Y. H., Kang, C. and Sohn, N. W. 2014. Alpha-asarone Ameliorates memory deficit in lipopolysaccharide-treated mice via suppression of pro-inflammatory cytokines and microglial activation. Biomol. Ther. 22, 17-26. crossref(new window)

Simon, H. U., Haj-Yehia, A. and Levi-Schaffer, F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis 5, 415-418. crossref(new window)

Taniyama, Y. and Griendling, K. K. 2003. Reactive oxygen species in the vasculature molecular and cellular mechanisms. Hypertension 42, 1075-1081. crossref(new window)

Vihinen, P. and Kähäri, V. M. 2002. Matrix metalloproteinases in cancer: prognostic markers and therapeutic targets. Int. J. Cancer 99, 157-166. crossref(new window)