Advanced SearchSearch Tips
Production and Characterization of Extracellular Polysaccharide Produced by Pseudomonas sp. GP32
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 25, Issue 9,  2015, pp.1027-1035
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.9.1027
 Title & Authors
Production and Characterization of Extracellular Polysaccharide Produced by Pseudomonas sp. GP32
Lee, Myoung Eun; Lee, Hyun Don; Suh, Hyun-Hyo;
  PDF(new window)
A strain GP32 which produces a highly viscous extracellular polysaccharide was conducted with soil samples and identified as Pseudomonas species. The culture flask conditions for the production of extracellular polysaccharide by Pseudomonas sp. GP32 were investigated. The most suitable carbon and nitrogen source for extracellular polysaccharide production were galactose and (NH4)2SO4. The optimum carbon/nitrogen ratio for the production of extracellular polysaccharide was around 50. The optimum pH and temperature for extracellular polysaccharide production was 7.5 and 32℃, respectively. In batch fermentation using a jar fermentor, the highest extracellular polysaccharide content (15.7 g/l) was obtained after 70 hr of cultivation. The extracellular polysaccharide produced by Pseudomonas sp. GP32 (designated Biopol32) was purified by ethanol precipitation, cetylpyridinium chloride (CPC) precipitation, and gel permeation chromatography. Biopol32, which has an estimated molecular weight of over 3×107 datons, is a novel polysaccharide derived from sugar components consisting of galactose, glucose, gulcouronic acid and galactouronic acid in an approximate molar ratio of 1.85 : 3.24 : 1.00 : 1.42. The solution of Biopol32 showed non-Newtonian characteristics. The viscosity of Biopol32 exhibited appeared to be higher at all concentration compared to that of zooglan from Zoogloea ramigera. An analysis of the flocculating efficiency of Biopol32 in industry wastewater (food, textile, and paper wastewater) revealed chemical oxygen demand (COD) reduction rates 58.4-67.3% and suspended solid (SS) removal rates 82.6-91.3%. Based on these results, Biopol32 is a possible candidate for industrial applications such as wastewater treatment.
Apparent viscosity;bacterial polysaccharide;flocculating efficiency;polysaccharide production;
 Cited by
Andrew, J. R., Drozd, J. W., Jones, C. W. and Linton, J. D. 1988. Growth efficiency of Xanthomonas compestris in continuous culture. J. Gen. Microbiology 134, 1055-1061.

Ashtaputre, A. A. and Shah, A. K. 1995. Studies on a viscous, gel-forming exopolysaccharide from Sphingomonas paucimobilis GS1. Appl. Environ. Microbiol. 61, 1159-1162.

Becker, A., Katzen, F., Puhler, A. and Lelpi, L. 1998. Xanthan gum biosynthesis and application : A biochemical/genetic prospective. Appl. Microbiol. Biotechnol. 50, 145-152. crossref(new window)

Chaplin, M. F. and Kennedy, J. F. 1968. Phenol-sulfuric acid assay. p. In M. F. Chaplin and J. F. Kennedy (eds), Carbohydrate Analysis : A Practical Approach. IRL Press,Washington, DC., U.S.A.

Chen, R. H., Ker, Y. C. and Wu, C. S. 1990. Temperature and shear rate affecting the viscosity and secondary structural changes soy 11S globulin measured by a cone-plate viscometer and fourier transform infrared spectroscopy. Agric. Biol. Chem. 54, 1165-1176. crossref(new window)

Crescenzi, V. 1995. Microbial polysaccharides of applied interest : On going research activities in Europe. Biotechnol. Prog. 11, 251-259. crossref(new window)

Dearfield, K. L. and Ambermathy. 1988. Acrylamide its metabolism, development and reproductive effects, genotoxicity, and carcinogenicity. Mutant Res. 195, 45-77.

Kennedy, J. F. and Bradshow. 1984. Progress in industrial microbiology. Bushell, M. E. (ed.) Elsevier. 19, 319-365.

Kurane, R., Takeda, K. and Suzuki, T. 1986. Screening for and characteristics of microbial flocculants. Agr. BIol. Chem. 50, 2301-2307. crossref(new window)

Kurane, R., Toeda, K., Takeda, K. and Suzuki, T. 1986. Culture conditions for production of microbial flocculant by Rhodococcus erythropolis. Agric. Biol. Chem. 50, 2309-2313. crossref(new window)

Lawson, C. J. and Shurtland, I. W. 1978. Polysaccharides, pp. 327-397, Rose, A. H. (ed), Economic Microbiology, vol. 2, Academic Press, New York.

MacFaddin, J. F. 1984. Biochemical tests for identification for medical bacteria. 2nd eds,.Williams & Wilkins Co., Baltimore, Maryland.

Margaritis, A. and Pace, G. W. 1985. Micorbial polysaccharides, pp. 1005-1044. In: Murray, M. Y. (ed.), Comprehensive Biotechnology, The principles, applications and regulations of biotechnology in industry, Agriculture and Medicine.

Marshall, K. and Weigel, H. 1980. Evidence of multiple branching in the levan elaborated by Streptococcus salivarius strain 51. Carbohyd. Res. 83, 321-326. crossref(new window)

McNeil, B. and Kristanian, B. 1989. Temperature effect on pulluan formation by Aureobasidium pullulans in stirred tanks. Enzyme Microb. Technol. 12, 521-526.

Moorhouse, R. 1987. In M. Yalpany (ed.), Industrial polysaccharide, Structure property relationships of family of microbial polysaccharides, pp.187-206. Elsevier, Amsterdam.

Nohata, Y. and Kurane, B. 1993. Culture conditions for production and purification of bioabsorbent from Acaligenes latus B-16. J. Ferment. Bioeng. 77, 390-393.

Peter, H. A. S., Nicholas, S. M., Sharpe, M. E. and Holt, J. F. 1986. Bergey’s manual of systematic bacteriology, Williams and Wikins Co., Baltimore, Maryland.

Rehm, B. H. A. and Valla, S. 1997. Bacterial alginates : Biosynthesis and application. Appl. MIcrobiol. Biotechnol. 48, 281-288. crossref(new window)

Seo, W. T., Kahang, G. G., Nam, S. H., Choi, H. H., Kim, S. W. and Park, Y. H. 1999. Isolation and characterization of a novel exopolsaccharide-producing Penibacillus sp. WN9 KCTC 8591P. J. Microbiol. Biotechnol. 9, 820-825.

Souw, P. and Demain, A. L. 1979. Nutritional studies on xanthan production by Xanthomonas compestris NRRL B1459. Appl. Environ. Microbiol. 37, 1186-1192.

Speer, R. A. and Tung, M. A. 1986. Concentration and temperature dependence of low behavior of xanthan gum dispersions. J. Food. Sci. 56, 96-103.

Tait, M. I. Sutherland, I. W. and Clarke-Sturman, A. 1986. Effect of growth condition on the production, composition and viscosity of Xanthomonas compestris exopolysaccharide. J. Gen. Microbiol. 132, 1483-1492.

Tamaoka, K. and Komagata, K. 1984. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25, 125-128. crossref(new window)

Takagi, H. and Kadowaki, K. 1985. Purification and chemical properties of a flocculant produced by Paecilomyces. Agric. Biol. Chem. 49, 3159-3164. crossref(new window)

Toeda, K. and Kurane, R. 1991. Microbial flocculant from Alcaligenes cupidus KT201. Agric. Biol. Chem. 55, 2793-2799. crossref(new window)

Unz, R. F. and Farrah, S. R. 1976. Exopolymer production and flocculation by Zoogloea MP6. Appl. Environ. Microbiol. 31, 623-626.

Williams, A. G. and Wimpenny, J. T. T. 1977. Exopolysaccharide production by Pseudomonas NCIB 11264 grown on batch culture. J. Gen. Microbiol. 102, 13-21. crossref(new window)