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Anticancer Effect of Citrus Fruit Prepared by Gamma Irradiation of Budsticks
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 9,  2015, pp.1051-1058
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.9.1051
 Title & Authors
Anticancer Effect of Citrus Fruit Prepared by Gamma Irradiation of Budsticks
Kim, Ji Hye; Kim, Min Young;
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 Abstract
Citrus mutant fruits were induced by irradiation of citrus budsticks with 120 Gy of cobalt (60CO) gamma irradiation. The citrus mutant inhibited the growth and induced apoptosis in various human cancer cells, including A549, HepG2, HCT116, MCF-7, and Hela. The results of a trypan blue exclusion assay showed that citrus mutant fruits exhibited excellent antiproliferation activity in various human cancer cells and low cytotoxicity in normal 16HBE140- and CHANG cells. In addition, the cell death induced by the citrus mutant fruits was associated with an increased population of cells in sub-G1 phase, and it caused DNA fragmentation in human lung adenocarcinoma A549 and hepatocellular carcinoma HepG2 cells. It also up-regulated the amount of cellular nitric oxide (NO) produced as a result of nitric oxide synthase (NOS) activation and suppressed the inhibitor of apoptosis protein (IAP) family in A549 and HepG2 cells. These findings indicate that the citrus mutant fruits activates the NO-mediated apoptotic pathway in A549 and HepG2 cells. It may merit further investigation as a potential chemotherapeutic and chemopreventive agent for the treatment of various types of cancer cells. The results provide important major new insights into the mechanisms of the anticancer activity of citrus mutant fruits.
 Keywords
Apoptosis;citrus mutant fruits;human cancer cells;nitric oxide;
 Language
Korean
 Cited by
1.
Antioxidant and Anti-Proliferative Activities of Oats under Different Solvent Extraction Conditions, Journal of the Korean Society of Food Science and Nutrition, 2016, 45, 6, 918  crossref(new windwow)
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