Advanced SearchSearch Tips
Inhibition of Inflammation by Popillia flavosellata Ethanol Extract in LPSinduced RAW264.7 Macrophages
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 25, Issue 9,  2015, pp.993-999
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.9.993
 Title & Authors
Inhibition of Inflammation by Popillia flavosellata Ethanol Extract in LPSinduced RAW264.7 Macrophages
Yoon, Young-Il; Hwang, Jae-Sam; Kim, Mi-Ae; Ahn, Mi Young; Lee, Young-Bo; Han, Myung Sae; Goo, Tae-Won; Yun, Eun-Young;
  PDF(new window)
The beetle Popillia flavosellata has been no reported its functional effects. In this study, we investigated the anti-inflammatory effect of P. flavosellata ethanol extract (PFE) on RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for the induction of inflammation. First, we examined the cytotoxicity of PFE in the RAW 264.7 cells at a concentration of 2,000 μg/ml or less. To evaluate the anti-inflammatory effects of PFE, we investigated the expression levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and proinflammatory enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether PFE inhibited the translocation of nuclear factor kappa B (NF-κB) p65 into the nucleus in the LPS-induced RAW 264.7 cells. We found that the protein levels of TNF-α and IL-6 were decreased in the LPS-induced RAW 264.7 cells after the treatment with PFE in a dose-dependent manner. In addition, we confirmed that PFE inhibited the translocation of NF-κB p65 into the nucleus, as well as the protein expression levels of iNOS and COX-2. Accordingly, we propose that PFE exerts an anti-inflammatory effect through the down-regulation of NF-κB p65, TNF-α, IL-6, iNOS, and COX-2 via the toll like receptor (TLR)-4 inflammatory signaling pathway.
Anti-inflammation;lipopolysaccharide;Popillia flavosellata;pro-inflammatory cytokine;RAW 264.7 cells;
 Cited by
Ahmadi-Ashtiani, H. R., Rastegar, H. and Arkam, M. 2013. The inhibitory effect of silymarin on cell viability and cellular COX-2 and iNOS level in HepG2 cell line. Health Med. 7, 2008-2014.

Arora, R. B., Kapoor, V., Basu, N. and Jain, A. P. 1971. Anti-inflammatory studies on Curcuma longa (turmeric). Indian J. Med. Res. 59, 1289-1295.

Baldwin, A. S. Jr. 1996. The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu. Rev. Immunol. 14, 649-683. crossref(new window)

Brennan, F. M., Chantry, D., Jackson, A., Maini, R. and Feldmann, M. 1989. Inhibitory effect of TNF alpha antibodies on synovial cell interleukin-1 production in rheumatoid arthritis. Lancet 2, 244-247.

Cerella, C., Sobolewski, C., Dicato, M. and Diederich, M. 2010. Targeting COX-2 expression by natural compounds:a promising alternative strategy to synthetic COX-2 inhibitors for cancer chemoprevention and therapy. Biochem. Pharmachol. 15, 1801-1815.

Chung, M. Y., Hwang, J. S., Goo, T. W. and Yun, E. Y. 2013. Analysis of general composition and harmful material of Protaetia brevitarsis. J. Life Sci. 23, 664-668. crossref(new window)

Ferrero-Miliani, L., Nielsen, O. H., Andersen, P. S. and Girardin, S. E. 2007. Chronic inflammation: Importance of NOD2 and NALP3 in interleukin-1beta generation. Clin. Exp. Immunol. 147, 227-235.

Gabay, C. 2006. Interleukin-6 and chronic inflammation. Arthritis Res. Ther. 8, S3.

Giuliani, C., Napolitano, G., Bucci, I., Montani, V. and Monaco, F. 2001. NF-κB transcription factor: role in the pathogenesis of inflammatory, autoimmune, and neoplastic diseases and therapy implications. Clin. Ter. 152, 249-253.

Hink, U. and Münzel, T. 2006. COX-2, another important player in the nitric oxide-endothelin cross-talk: good news for COX-2 inhibitors? Circ. Res. 98, 1344-1346. crossref(new window)

Howes, L. G. 2007. Selective COX-2 inhibitors, NSAIDs and cardiovascular events - is celecoxib the safest choice? Ther. Clin. Risk Manag. 3, 831-845.

Iwalewa, E. O., McGaw, L. J., Naidoo, V. and Eloff, J. N. 2007. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of south african origin used to treat pain and inflammatory conditions. Afr. J. Biotechnol. 6, 2868-2885.

Kang, C. H., Choi, Y. H., Choi, I. W., Lee, J. D. and Kim, G. Y. 2011. Inhibition of lipopolysaccharide-induced iNOS, COX-2, and TNF-α expression by aqueous extract of Orixa Japonica in RAW 264.7 cells via suppression of NF-κB activity. Trop. J. Pharm. Res. 10, 161-168.

Kim, J. J., Jang, B. K., Lee, S. M., Choo, H. Y., Zhu, M. and Lee, D. W. 2011. Investigation of host plants and seasonal occurrence of Popillia flavosellata (Coleoptera: Rutelidae) ingolf courses. Asian J. Turfgrass Sci. 25, 22-29.

Lee, S. Y., Kwon, H. K. and Lee, S. M. 2011. SHINBARO, a new herbal medicine with multifunctional mechanism for joint disease: first therapeutic application for the treatment of osteoarthritis. Arch. Pharm. Res. 34, 1773-1777. crossref(new window)

Miyataka, M., Rich, K. A., Ingram, M., Yamamoto, T. and Bing, R. J. 2002. Nitric oxide, anti-inflammatory drugs on renal prostaglandins and cyclooxygenase-2. Hypertension 39, 785-789. crossref(new window)

Mitchell, J. A., Akarasereenont, P., Thiemermann, C., Flower, R. J. and Vane, J. R. 1993. Selectivity of nonsteroid anti-inflammatory drugs as inhibitors of constitutive and inducible cyclo-oxygenase. Proc. Nat. Acad. Sci. USA 90, 11693-11697. crossref(new window)

Monaco, C., Andreakos, E., Kiriakidis, S., Mauri, C., Bicknell, C., Foxwell, B., Cheshire, N., Paleolog, E. and Feldmann, M. 2004. Canonical pathway of nuclear factor kappa B activation selectively regulates proinflammatory and prothrombotic responses in human atherosclerosis. Proc. Nat. Acad. Sci. USA 101, 5634-5639. crossref(new window)

Nakanishi, M. and Rosenberg, D. W. 2013. Multifaceted roles of PGE2 in inflammation and cancer. Semin. Immunopathol. 35, 123-137. crossref(new window)

Paek, M. K., Hwang, J. M., Jung, K. S., Kim, T. W., Kim, M. C., Lee, Y. J., Cho, Y. B., Park, S. W., Lee, H. S., Ku, D. S., Jeong, J. C., Kim, K. G., Choi, D. S., Shin, E. H., Jwang, J. H., Lee, J. S., Kim, S. S. and Bea, Y. S. 2010. Checklist of Korean insects. Nature & Ecology, pp. 113.

Park, J. Y., Heo, J. C., Woo, S. U., Yun, C. Y., Kang S. W., Hwang, J. S. and Lee, S. H. 2006. Anti-inflammatory and cellular protective effects on hydrogen peroxide-induced cytotoxicity of grasshopper extracts. Kor. J. Food Preserv. 13, 796-802.

Rajendram, R., Saraswathy, S. and Rao, N. A. 2007. Photoreceptor mitochondrial oxidative stress in early experimental autoimmune uveoretinitis. Br. J. Ophthalmol. 91, 531-537. crossref(new window)

Rao, C. V., Indranie, C., Simi, B., Manning, P. T., Connor, J. R. and Reddy, B. S. 2002. Chemopreventive properties of a selective inducible nitric oxide synthase inhibitor in colon carcinogenesis, administered alone or in combination with celecoxib, a selective cyclooxygenase-2 inhibitor. Cancer Res. 62, 165-170.

Medzhitov, R. 2008. Origin and physiological roles of inflammation. Nature 454, 428-435. crossref(new window)

Salvemini, D., Milko, T. P., Masferrer, J. L., Seibert, K., Currie, M. G. and Needleman, P. 1993. Nitric oxide activates cyclooxygenase enzymes. Proc. Nat. Acad. Sci. USA 90, 7240-7244. crossref(new window)

Shafran, Y., Zurgil, N., Afrimzon, E., Tauber, Y., Sobolev, M., Shainberg, A. and Deutsch, M. 2012. Correlative analyses of nitric oxide generation rates and nitric oxide synthase levels in individual cells using a modular cell-retaining device. Anal. Chem. 84, 7315-7322. crossref(new window)

Tak, P. P. and Firestein, G. S. 2001. NF-kappaB: a key role in inflammatory diseases. J. Clin. Invest. 107, 7-11. crossref(new window)

Tracey, D., Klareskog, L., Sasso, E. H., Salfeld, J. G. and Tak, P. P. 2008. Tumor necrosis factor antagonist mechanisms of action: a comprehensive review. Pharmacol. Ther. 117, 244-279. crossref(new window)

Swierkosz, T. A., Mitchell, J. A., Warner, T. D., Botting, R. M. and Vane, J. R. 1995. Co-induction of nitric oxide synthase and cyclo-oxygenase: interactions between nitric oxide and prostanoids. Br. J. Pharmacol. 114, 1335-1342. crossref(new window)

Van Huis, A., Van ltterbeeck, J., Klunder, H., Mertens, E., Halloran. A., Muir, G. and Vantomme, P. 2013. Edible in sects:future prospects for food and feed security. Food and agriculture Organization of the United Nations, Rome, Italy.

Vane, J. R. 1971. Inhibition of prostaglandin synthesis as a mechanism of action for the aspirin-like drugs. Nature 231, 232-235.

Vlassara, H. 2005. Advanced glycation in health and disease role of the modern environment. Ann. N. Y. Acad. Sci. 1043, 452-460. crossref(new window)

Wang, X., Luo, Y., Liao, W. B., Zhang, J. and Chen, T. M. 2013. Effect of osteoprotegerin in combination with interleukin-6 on inhibition of osteoclast differentiation. Chin. J. Traumatol. 16, 277-280.

Yoo, J. M., Hwang, J. S., Goo, T. W. and Yun, E. Y. 2013. Comparative analysis of nutritional and harmful components in Korean and Chinese mealworms (Tenebrio molitor). J. Kor. Soc. Food Sci. Nutr. 42, 249-254. crossref(new window)

Yoon, Y. I., Chung, M. Y., Hwang, J. S., Goo, T. W., Ahn, M. Y., Lee, Y. B., Han, M. S. and Yun, E. Y. 2014. Anti-inflammatory effect of Oxya chinensis sinuosa ethanol extract in LPS-induced RAW 264.7 cells. J. Life Sci. 24, 370-376. crossref(new window)