Advanced SearchSearch Tips
Ulmus macrocarpa Hance Water Extract Improved Splenocytes Survival and NK Cell Cytotoxicity
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 26, Issue 1,  2016, pp.109-116
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.1.109
 Title & Authors
Ulmus macrocarpa Hance Water Extract Improved Splenocytes Survival and NK Cell Cytotoxicity
Lee, Sung Do; Kim, Deok Won; Lee, Inhwan; Lee, Jong-Hwan; Hyun, Sook Kyung; Kang, Kyung-Hwa; Hwang, HyeJin; Kim, CheolMin; Kim, Byoung Woo; Chung, Kyung Tae;
  PDF(new window)
Ulmi cortex is the elm bark or root bark of Ulmus macrocarpa Hance and has been used as an ingredient of traditional medicine for anti-inflammatory, analgesic, anti-cancer and wound healing on both the East and the West. This study investigated whether the Ulmus macrocarpa Hance Water extract (UMWE) has the in vivo and in vitro immune activating effect. Animals were orally administrated for 14 days as follows: no treat group with distilled water, cyclophosphamide (CY) group with 120 mg/kg of CY, UMWE 100+CY group with 100 mg/kg of UMWE and 120 mg/kg of CY, UMWE 200+CY group with 200 mg/kg of UMWE and 120 mg/kg of CY, UMWE 100 group with 100 mg/kg of UMWE and UMWE 200 group with 200 mg/kg of UMWE. The immunosuppressive drug CY was intraperitoneally injected to induce immune suppression. Spleen indices showed small changes in CY injected groups but splenocyte indices showed greater decrease in the same groups. However, UMWE appeared to relieve CY’s immunosuppression. UMWE also delayed in vitro splenocyte death increasing its longevity. These data obtained by MTT assay and 7-amino-actinomycin D which stains preferentially dead than live cells. UMWE alone did not show cytotoxicity based on its apoptototic effect on splenocytes in vitro and in vivo. Splenic NK cell activity was maintained by UMWE under the presence of CY in vitro. The data indicated that UMWE protects splenocytes from the immunosuppressive drug CY under in vitro and in vivo conditions.
Immune function;NK cell activity;splenocyte survival;cyclophosphamide;Ulmus macrocarpa Hance;
 Cited by
Chemical Characteristics and Immunostimulating Activity of Crude Polysaccharide Isolated from Commercial Instant Coffee, Korean Journal of Food Science and Technology, 2016, 48, 3, 289  crossref(new windwow)
Choi, W. H., Oh, Y. S., Ahn, J. Y., Kim, S. R. and Ha, T. Y. 2005. Antioxidative and protective effect of Ulmus davidiana var. japonica extracts on glutamate-induced cytotoxicity in PC 12 cells. Kor. J. Food Scl. Technol. 37, 479-483.

Cesta, M. F. 2006. Normal structure, function, and histology of the spleen. Toxicol. Pathol. 34, 455-465. crossref(new window)

Collins, F. M., Congdon, C. C. and Morrison, N. E. 1975. Growth of Mycobacterium bovis (BCG) in T lymphocyte-depleted mice. Infect. Immun. 11, 57-64.

Dranoff, G. 2004. Cytokines in cancer pathogenesis and cancer therapy. Nature Reviews Cancer 4, 11-22. crossref(new window)

Emadi, A. Jones, R. J. and Brodsky, R. A. 2009. Cyclophosphamide and cancer: golden anniversary. Nat. Rev. Clin. Oncol. 6, 638-647. crossref(new window)

Fetterhoff, T. J., Holland, S. P. and Wile, K. J. 1993. Fluorescent detection of non-viable cells in fixed cell preparations. Cytometry Suppl. 6, 27.

Goldstein, M. Roos, W. P. and Kaina, B. 2008. Apoptotic death induced by the cyclophosphamide analogue mafosfamide in human lymphoblastoid cells: contribution of DNA replication, transcription inhibition, and Chk/p53 signaling. Toxicol. Appl. Pharmacol. 229, 20-32. crossref(new window)

Huang, G. C., Wu, L. S., Chen, L. G., Yang, L. L. and Wang, C. C. 2007. Immuno-enhancement effects of Huang Qi Liu Yi Tang in a murine model of cyclophosphamide-induced leucopenia. J. Erthnopharmacol. 109, 229-235. crossref(new window)

Huyan, X. H., Lin, Y. P., Gao, T., Chen, R. Y. and Fan, Y. M. 2011. Immunosuppressive effect of cyclophosphamide on white bloodcells and lymphocyte subpopulations from peripheral blood of Balb/c mice. International Immunopharmacol. 11, 1293-1297. crossref(new window)

Hamerman, J. A., Ogasawara, K. and Lanier, L. L. 2005. NK cells in innate immunity. Current Opinion in Immunol. 17, 29-35. crossref(new window)

Jeong, J. T. 2007. An analysis of the research of pharmacological effects for the ulmus davidiana planch. dissertation, Chosun University, Gwangju, Korea.

Jeong, K. Y. and Kim, M. L. 2012. Physiological activities of Ulmus pumila L. extracts. Kor. J. Food Preserv. 19, 104-109. crossref(new window)

Kim, K. H., Kang, I. K., Kang, E. J., Yang, E. K. and Park, S. N. 2004. A research trend of natural product on well-being industry. J. Soc. Cosmet. 30, 329-343.

Koopman, G., Reutelingsperger, C. P. M., Kuijten, G. A. M., KeeHnen, R. M. J., Pals, S. T. and van Oers, M. H. J. 1994. Annexine V for flow cytometric detection of phosphatidylserine expression on B Cells undergoing apoptosis. Blood. 84, 1415-1420.

Lee, Y. J. and Han, J. P. 2000. Antioxidative activities and nitrite scavenging abilities of extracts from Ulmus devidiana. J. Kor. Soc. Food Sci. Nutr. 29, 893-899.

Lee, S. E., Kim, Y. S., KIm, J. E., Bang, J. K. and Seong, N. S. 2004. Antioxidant activity of Ulmus davidiana var.japonica N. and Hemipteleae davidii P. Kor. J. Medicinal Crop. Sci. 12, 321-327.

Lee, E. H., Park, C. W. and Jung, Y. J. 2013. Anti-inflammatory and immune-modulating effect of Ulmus davidiana var. japonica Nakai extract on a macrophage cell line and immune cells in the mouse small intestine. J. Ethnopharmacol. 146, 608-613. crossref(new window)

Lee, H. Y., Kim, C. K., Sung, T. K., Mun, T. K. and Lim, C. J. 1992. Antibacterial activity of Ulmus pumila L. extract. Kor. J. Appl. Microbiol. Biotechnol. 20, 1-5.

Lee, K. H., Cho, C. H. and Yoon, W. H. 2004. In vivo antitumor activity of mansonone E isolated from Ulmus davidiana var. japonica NAKAI. Kor. J. Pharmacogn. 35, 199-202.

Lafreniere, R. and Rosenberg, S. A. 1985. Successful immunotherapy of murine experimental hepatic metastases with lymphokine-activated killer cells and recombinant interleukin 2. Cancer Res. 45, 3735-3741.

Leung, H. Y., Ma, C. W., Tang, Q. T. and Ko, K. M. 2011. Long-Term treatment with a compound polysaccharide-based health product (InfinitusPolysac Plus) enhances innate and adaptive immunity in mice. Chinese Medicine 2, 178-185. crossref(new window)

Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63. crossref(new window)

Pak, C. H. 1999. Korean Folk Medicine, pp. 53, PNU Press, Korea.

Park, J. S., Shim, C. J., Jung, J. H., Lee, G. H., Sung, C. K. and Oh, M. J. 1999. Antimicrobial activity of ulmi cortex extracts. J. Kor. Soc. Food Sci. Nutr. 28, 1022-1028.

Parham, P. 2009. The Immune System, pp 21-22, 3rd edition. Garland Science: New York, NY, USA.

Seo, B. I., Ju, Y. S., Choi, H. Y., Park, Roh, S. S., Koo, J. S., Kim, J. J. and Kim, D. Y. 2011. Illustrated Book of Herbal Plants in Oriental Medicine, pp. 353-357, DaeWondang, Korea.

Schmid, I., Uittenbogaart, C. H., Krall, W. J., Braun, J. and Giorgi, J. V. 1992. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13, 204-208. crossref(new window)

Shimizu, S. 2004. The Laboratory mouse. Elsevier.

Wang, J., Tong, X., Li, P., Cao, H. and Su, W. 2012. Immuno-enhacement effects of ShenqiFuzheng Injection on cyclophosphamide-induced immunosuppression in Balb/c mice. J. Ethnopharmacol. 139, 769-775.

Winkelstein, A. 1973. Mechanism of Immunosuppression : Effects of Cyclophosphamide on Cellular Immunity. Blood 41, 273-284.

Yang, Y. L., Kim, Y. J., Kim, K. H. and Oh, E. G. 2001. Separation of glycoprotein and its anticancer immunostimulating activity from dried barks of slippery elm [Ulmus parvifolia]. Kor. J. Biotechnol. Bioeng. 16, 547-553.

Yang, S. X. and Li, X. Yu. 1990. Enhancement of T lymphocyte proliferation and suppression of antibody producing cell formation by methionine-enkephalin. Acta Pharmacologica Sinica 11, 355-359.