Advanced SearchSearch Tips
Suppressive Effects of Lees from Sweet Potato Soju on LPS-induced Inflammatory Responses in RAW 264.7 Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 26, Issue 1,  2016, pp.117-122
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.1.117
 Title & Authors
Suppressive Effects of Lees from Sweet Potato Soju on LPS-induced Inflammatory Responses in RAW 264.7 Cells
Lee, Seung-Hoon; Kwon, Min-Jeong; Kim, Soon Young; Sohn, Ho-Yong; Shin, Woo-Chang; Kim, Jong-Sik;
  PDF(new window)
In the current study, the ethanol extracts and their subsequent organic solvent fractions from lees of sweet potato soju were prepared and the prepared samples were designated as from KSD-E8-1 to KSD-E8-5. Their effects on cell viability and nitric oxide (NO) production in mouse macrophage RAW 264.7 cells were investigated. The results showed that the ethyl acetate fraction (KSD-E8-3) of lees extracts from sweet potato soju significantly decreased nitric oxide (NO) production in LPS-activated RAW 264.7 cells, whereas they did not affect cell viabilities. The fraction KSD-E8-3 reduced the expression of pro-inflammatory genes such as COX-2, iNOS and TNF-alpha and also decreased protein expression of iNOS in a dose dependent manner, which were detected with RT-PCR and Western blot analysis, respectively. In addition, we detected the expression of mitogen-activated protein kinases (MAPKs) such as p38, JNK, and ERK1/2 and their phosphorylated forms. The results indicated that the treatment of the fraction KSD-E8-3 did inhibit phosphorylation of p38, JNK, and ERK1/2 MAPKs, indicating that the fraction KSD-E8-3 regulates LPS-induced inflammatory response via suppressing MAPK signaling pathway. Overall, these results may contribute to understand the molecular mechanism of anti-inflammatory effects by the ethyl acetate fraction of lees extracts from sweet potato soju.
Anti-inflammation;lees extracts;organic solvent fractions;RAW 264.7 cell;sweet potato soju;
 Cited by
Akira, S. and Takeda, K. 2004. Toll-like receptor signaling. Nat. Rev. Immunol. 4, 499-511. crossref(new window)

Anderson, N. and Borlak, J. 2008. Molecular mechanisms and therapeutic targets in steatosis and steatoheptitis. Pharmacol. Rev. 60, 311-357. crossref(new window)

Chelsky, Z. L., Yue, P., Kondratyuk, T. P., Paladino, D., Pezzuto, J. M., Cushman, M. and Turkson, J. 2015. A resveratrol analogue promotes ERK MAPK-dependent STAT3 serine and tyrosine phosphorylation alterations and antitumor effects in vitro against human tumor cells. Mol. Pharmacol. 88, 524-533. crossref(new window)

Eisinger, A. L., Prescott, S. M., Jones, D. A. and Stafforini, D. M. 2007. The role of cyclooxygenase-2 and prostaglandins in colon cancer. Prostaglandins Other Lipid Mediat. 82, 147-154. crossref(new window)

Jacobsen, M. C., Dusart, P. J., Korowicz, K., Bajaj-eliott, M., Hart, S. L., Klein, N. J. and Dixon, G. L. 2015. A critical role for ATF2 transcription factor in the regulation of E-selectin expression in response to non-endotoxin components of Neisseria meningitides. Cell. Microbiol. DOI: 10.1111/cmi.12483. crossref(new window)

Jin, J. H., Kim, J. S., Kang, S. S., Son, K. H., Chang, H. W. and Kim, H. P. 2010. Anti-inflammatory and anti-arthritic activity of the roots of sophora flavescens. J. Ethnopharmacol. 127, 589-595. crossref(new window)

Ju, A., Cho, Y. C. and Cho, S. 2015. Methanol extracts of Xanthium sibiricum roots inhibit inflammatory response via the inhibition of nuclear factor-κB (NF-κB) and signal transducer and activator of transctiption 3 (STAT3) in murine macrophage. J. Ethnopharmacol. DOI: 10.1016/j.jep.2015.07.038. crossref(new window)

Kang, H. T., Lee, S. H., Kim, S. Y., Kim, M. S., Shin, W. C., Sohn, H. Y. and Kim, J. S. 2014. Anti-proliferative activities of solvent fractions of lees extracts in human colorectal HCT 116 ells. J. Life Sci. 24, 967-972. crossref(new window)

Kim, H. G., Shi, C., Bode, A. M. and Dong, Z. 2015. p38α MAPK is required for arsenic-induced cell transformation. Mol. Carcinog. DOI: 10.1002/mc.22331. crossref(new window)

Kim, M. S., Lee, Y, S., Kim, J. S., Shin, W. C. and Sohn, H. Y. 2014. Evaluation of in-vitro antithrombosis activity of lees of Korean traditional wine. J. Life Sci. 24, 865-872. crossref(new window)

Kindt, T. J., Goldsby, R. A. and Osborne, B. A. 2007. Innate immunity : Tenneys. Kuby Immunology. New York. Freeman Press 6, 52-73.

Kim, T. Y., Jeon, T. W., Yeo, S. H., Kim, S. B., Kim, J. S. and Kwak, J. S. 2010. Antimicrobial, antioxidant and SOD-like activity effect of Jubak extracts. Kor. J. Food Nutr. 23, 299-305.

Kojda, G. and Harrison, D. 1999. Interactions between NO and reactive oxygen speices : pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc. Res. 43, 562-571. crossref(new window)

Lee, E. S., Ju, H. K., Moon, T. C., Lee, E., Jahng, Y., Lee, S. H. and Chang, H. W. 2004. Inhibition of nitric oxide and tumor necrosis factor-alpha (TNF-alpha) production by prepenone compound through blockade of nuclear factor (NF)-κB activation in cultured murine macrophages. Biol. Pharm. Bull. 27, 617-620. crossref(new window)

Li, G., Wulan, H., Song, Z., Paik, P. A., Tsao, M. L., Goodman, G. M., MacEachern, P. T., Downey, R. S., Jankowska, A. J., Rabinowitz, Y. M., Learch, T. B., Song, D. Z., Yuan, J. J., Zheng, S. and Zheng, Z. 2015. Regulatory B cell function is suprresed by smoking and obesity in H. pylori-infected subjects and is correlated with elevated risk of gastric cancer. Plos One 10, e0134591. crossref(new window)

Lo, J. Y., Kamarudin, M. N., Hamdi, O. A., Awang, K. and Kadir, H. A. 2015. Curcumenol isolated from Curuma zedoaria suppresses Akt-mediated NF-κB activation and p38 MAPK signaling pathway in LPS-stimulated BV-2 microlial cells. Food Funct. 6, 3550-3559. crossref(new window)

Monick, M. M., Arter, A. B., Robeff, P. K., Flaherty, D. M., Peterson, M. W. and Hunninghake, G. W. 2001. Lipopolysaccharide activates Akt in human alveolar macrophages resulting in nuclear accumulation and transcriptional activity of beta-catenin. J. Immunol. 166, 4713-4720. crossref(new window)

Park, M. J., Kang, H. T., Kim, M. S., Shin, W. C., Sohn, H. Y. and Kim, J. S. 2014. Anti-inflammatory effects of extracts and their solvent fractions of rice wine lees. J. Life Sci. 24, 843-850. crossref(new window)

Sahu, B. D., Kumar, J. M. and Sistla, R. 2015. Baicalein, a bioflavonoid, prevents cisplatin-induced acute kidney injury by up-regulating antioxidant defense and down-regulating the MAPKs and NF-κB pathways. Plos One 10, e0134139. crossref(new window)

Syed Hussein, S. S., Kamarudin, M. N. and Kadir, H. A. 2015. (+)-Catechin attenuates NF-κB activation through regulation of Akt, MAPK, and AMPK signaling pathways in LPS-induced BV-2 microglial cells. Am. J. Chin. Med. 43, 927-952. crossref(new window)

Vlkova, M., Ticha, O., Nechvatalova, J., Kalina, T., Litzman, J., Mauri, C. and Blair, P. A. 2015. Regulatory B cells in CVID patients fail to suppress multifunctional IFN-gamma+TNF-alpha+CD4+ T cells differentiation. Clin. Immunol. 29, 292-300.

Xu, C., Chen, G., Yang, W., Xu, Y., Xu, Y., Huang, X., Liu, J., Feng, Y., Xu, Y. and Liu, B. 2015. Hyaluronan ameliorates LPS-induced acute lung injury in mice via Toll-like receptor (TLR) 4-dependent signaling pathway. Int. Immunopharmacol. 28, 1050-1058. crossref(new window)