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Extract from Eucheuma cottonii Induces Apoptotic Cell Death on Human Osteosarcoma Saos-2 Cells via Caspase Cascade Apoptosis Pathway
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 2,  2016, pp.147-154
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.2.147
 Title & Authors
Extract from Eucheuma cottonii Induces Apoptotic Cell Death on Human Osteosarcoma Saos-2 Cells via Caspase Cascade Apoptosis Pathway
Kang, Chang-Won; Kang, Min-Jae; Kim, Kyong Rok; Kim, Nan-Hee; Seo, Yong Bae; Kang, Keon-Hee; Kim, Sang-Ho; Kim, Gun-Do;
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 Abstract
Osteosarcoma (OS) is the most common and malignant bone tumors. Although many types of resection surgery and experimental agents were developed, median survival and clinical prognosis are poorly investigated. Recently, several researches have reported that Eucheuma cottonii has potent as protective effects of coal dust-induced lung damage via inhibition of malondialdehyde (MDA) and oxidative stress in bronchoalveolar lavage fluids (BALF). However, anti-cancer effects and specific molecular mechanism of extract from Eucheuma cottonii (EE) has not been clearly studied yet. This study evaluated that anti-cancer potential of EE in human osteosarcoma Saos-2 cells. EE indicated cytotoxicity on Saos-2 cells in a dose-dependent manner. Morphological degradation and nucleic condensation were also observed under the EE treatment. However, it did not significantly affect on non-cancerous kidney HEK-293 cells under the same concentration which is shown cytotoxicity on Saos-2 cells. The phosphorylation of Fas-Associated Death Domain (FADD) and expression of cleaved caspase-8, -7 and -3 were upregulated in a dose-dependent manner. In immunofluorescence staining, expression level of Fas and cleaved PARP were upregulated by EE treatment. Furthermore, treatment of EE induces upregulation of sub G1 phase by flow cytometry analysis. The results demonstrated that EE has a therapeutic potential against osteosarcoma via FADD mediated caspase cascade apoptosis signal pathway.
 Keywords
Apoptosis;Eucheuma cottonii;FADD;Saos-2 cells;sub G1 phase;
 Language
Korean
 Cited by
 References
1.
Bard, D. R., Dickens, M. J., Smith, A. U. and Zarek, J. M. 1972. Isolation of living cells from mature mammalian bone. Nature 236, 314-315. crossref(new window)

2.
Bazzoni, F. and Beutler, B. 1996. The tumor necrosis factor ligand and receptor Families. N. Engl. J. Med. 334, 1717-1725. crossref(new window)

3.
Bertrand, T. E., Cruz, A., Binitie, O., Cheong, D. and Letson, G. D. 2015. Do surgical margins affect local recurrence and survival in extremity, nonmetastatic, high-grade osteosarcoma? Clin. Orthop. Relat. Res. PMID 26013153.

4.
Bielack, S. S., Kempf-Bielack, B., Delling, G., Exner, G. U., Flege, S., Helmke, K., Kotz, R., Salzer-Kuntschik, M., Werner, M. and Winkelmann, W. et al. 2002. Prognostic factors in high-grade osteosarcoma of the extremities or trunk: an analysis of 1,702 patients treated on neoadjuvant cooperative osteosarcoma study group protocols. J. Clin. Oncol. 20, 776-790. crossref(new window)

5.
Budihardjo, I., Oliver, H., Lutter, M., Luo, X. and Wang, X. 1999. Biochemical pathways of caspase activation during apoptosis. Annu. Rev. Cell Dev. Biol. 15, 269-290. crossref(new window)

6.
Cha, S. S., Sung, B. J., Kim, Y. A., Song, Y. L., Kim, H. J., Kim, S., Lee, M. S. and Oh, B. H. 2000. Crystal structure of TRAIL-DR5 complex identifies a critical role of the unique frame insertion in conferring recognition specificity. J. Biol. Chem. 275, 31171-31177. crossref(new window)

7.
Choudhuri, T., Pal, S., Agwarwal, M. L., Das, T. and Sa, G. 2002. Curcumin induces apoptosis in human breast cancer cells through p53-dependent Bax Induction. FEBS Lett. 512, 334-340. crossref(new window)

8.
Cohen, G. M. 1997. Caspases: The executioners of apoptosis. Biochem. J. 326, 1-16. crossref(new window)

9.
Creagh, E. M., Conroy, H. and Martin, S. J. 2003. Caspase-activation pathways in apoptosis and immunity. Immunol. Rev. 193, 10-21. crossref(new window)

10.
Czekanska, E. M., Stoddart, M. J., Richards, R. G. and Hayes, J. S. 2012. In search of an osteoblast cell model for in vitro research. Eur. Cell. Mater. 24, 1-17.

11.
Davis, A. M., Bell, R. S. and Goodwin, P. J. 1994. Prognostic factors in osteosarcoma: a critical review. J. Clin. Oncol. 12, 423-431.

12.
Earnshaw, W. C., Martins, L. M. and Kaufmann, S. H. 1999. Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu. Rev. Biochem. 68, 383-424. crossref(new window)

13.
Errami, Y., Naura, A. S., Kim, H., Ju, J., Suzuki, Y., El-Bahrawy, A. H., Ghonim, M. A., Hemeida, R. A., Mansy, M. S. and Zhang, J. et al. 2013. Apoptotic DNA fragmentation may be a cooperative activity between caspase-activated Deoxyribonuclease and the Poly (ADP-Ribose) polymerase- regulated DNAS1L3, an endoplasmic reticulum-localized endonuclease that translocates to the nucleus during apoptosis. J. Biol. Chem. 288, 3460-3468. crossref(new window)

14.
Isakoff, M. S., Bielack, S. S., Meltzer, P. and Gorlick, R. 2015. Osteosarcoma: current treatment and a collaborative pathway to Success. J. Clin. Oncol. 30, 3029-3035

15.
Kania, N., Mayangsari, E., Setiawan, B., Nugrahenny, D., Tony, F., Wahyuni, E. S. and Widodo, M. A. 2013. The effects of Eucheuma cottonii on signaling pathway inducing mucin synthesis in rat lungs chronically exposed to particulate matter 10 (PM10) coal dust. J. Toxicol. 2013, 528146.

16.
Kroemer, G., Galluzzi, L. and Brenner, C. 2007. Mitochondrial membrane permeabilization in cell death. Physiol. Rev. 87, 99-163. crossref(new window)

17.
Lazebnik, Y. A., Kaufmann, S. H., Desnoyers, S., Poirier, G. G. and Earnshaw, W. C. 1994. Cleavage of poly (ADP-Ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346-347. crossref(new window)

18.
Liu, X., Li, P., Widlak, P., Zou, H., Luo, X., Garrard, W. T. and Wang, X. 1998. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc. Natl. Acad. Sci. USA 95, 8461-8466. crossref(new window)

19.
Longhi, A., Errani, C., De Paolis, M., Mercuri, M. and Bacci, G. 2006. Primary bone osteosarcoma in the pediatric age: state of the Art. Cancer Treat. Rev. 32, 423-436. crossref(new window)

20.
Manivannan, K., Karthikai Devi, G., Anantharaman, P. and Balasubramanian, T. 2011. Antimicrobial potential of selected brown seaweeds from vedalai coastal waters, Gulf of Mannar. Asian Pac. J. Trop. Biomed. 1, 114-120. crossref(new window)

21.
Matanjun, P., Mohamed, S., Muhammad, K. and Mustapha, N. M. 2010. Comparison of cardiovascular protective Effects of tropical seaweeds, kappaphycus alvarezii, caulerpa lentillifera, and sargassum polycystum, on high-cholesterol/high-fat diet in rats. J. Med. Food 13, 792-800. crossref(new window)

22.
McIlwain, D. R., Berger, T. and Mak, T. W. 2013. Caspase functions in cell death and disease. Cold Spring Harb Perspect. Biol. 5, a008656.

23.
O′Kane, G. M., Cadoo, K. A., Walsh, E. M., Emerson, R., Dervan, P., O′Keane, C., Hurson, B., O′Toole, G., Dudeney, S. and Kavanagh, E. et al. 2015. Perioperative chemotherapy in the treatment of osteosarcoma: a 26-year single institution Review. Clin. Sarcoma Res. 5, 17-015-0032-0. eCollection 2015. crossref(new window)

24.
Prokhorova, E. A., Zamaraev, A. V., Kopeina, G. S. and Zhivotovsky, B. Lavrik, I. N. 2015. Role of the nucleus in apoptosis: signaling and execution. Cell Mol. Life Sci. 72, 4593-4612. crossref(new window)

25.
Roos, W. P. and Kaina, B. 2006. DNA damage-induced cell death by apoptosis. Trends Mol. Med. 12, 440-450. crossref(new window)

26.
Saputri, R. K., Setiawan, B., Nugrahenny, D., Kania, N., Wahyuni, E. S. and Widodo, M. A. 2014. The effects of Eucheuma cottonii on alveolar macrophages and malondialdehyde levels in bronchoalveolar lavage fluid in chronically particulate matter 10 coal dust-exposed rats. Iran. J. Basic Med. Sci. 17, 541-545.

27.
Schulze-Osthoff, K., Ferrari, D., Los, M., Wesselborg, S. and Peter, M. E. 1998. Apoptosis signaling by death receptors. Eur. J. Biochem. 254, 439-459. crossref(new window)

28.
Shamsabadi, F. T., Khoddami, A., Fard, S. G., Abdullah, R., Othman, H. H. and Mohamed, S. 2013. Comparison of Tamoxifen with edible seaweed (Eucheuma cottonii L.) extract in suppressing breast Tumor. Nutr. Cancer 65, 255-262. crossref(new window)

29.
Singh, N., Hassan, A. and Bose, K. 2015. Molecular basis of death effector domain chain assembly and its role in caspase-8 activation. FASEB J. 30, 186-200.

30.
Thompson, C. B. 1995. Apoptosis in the pathogenesis and treatment of disease. Science 267, 1456-1462. crossref(new window)

31.
Yang, J., Liu, X., Bhalla, K., Kim, C. N., Ibrado, A. M., Cai, J., Peng, T. I., Jones, D. P. and Wang, X. 1997. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science 275, 1129-1132. crossref(new window)

32.
Yoon, J. M., Koppula, S., Huh, S. J., Hur, S. J. and Kim, C. G. 2015. Low concentrations of doxycycline attenuates FasL- induced apoptosis in HeLa Cells. Biol. Res. 48, 38. crossref(new window)

33.
Zhang, J., Zhang, D. and Hua, Z. 2004. FADD and its phosphorylation. IUBMB Life 56, 395-401. crossref(new window)