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Characterization of Agarase Produced from the Isolated Marine Bacterium Marinomonas sp. SH-2
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 2,  2016, pp.198-203
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.2.198
 Title & Authors
Characterization of Agarase Produced from the Isolated Marine Bacterium Marinomonas sp. SH-2
Jo, Jeong-Gwon; Lee, Sol-Ji; Lee, Dong-Geun; Lee, Sang-Hyeon;
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This study aimed to isolate a novel agarase-producing marine bacterium and characterize its agarase, as agarases are known to produce biofunctional agarooligosaccharides or neo-agarooligosaccharides. A novel agar-degrading bacterium, SH-2, was isolated from the seawater of Namhae in Gyeongnam Province, Korea, and cultured in Marine agar 2216 medium. The 16S rRNA gene sequence represented 99% identity with that of the members of the Marinomonas genus; hence, the isolated bacterium was named Marinomonas sp. SH-2. The crude agarase was prepared from a culture medium of Marinomonas. sp SH-2, and exhibited maximum agarase activity at 170.2 units/l. The optimum conditions were pH 6.0 and 30℃ in 20 mM Tris-HCl buffer. The agarase activity of the bacterium was highly elevated from 20℃(42% relative activity) to 30℃(100%), and 82% activity was shown at 40℃. Its relative activities were less than 40% at over 40℃ after a 0.5 hr exposure. Relative activity was 100% at pH 6.0, while it was 72% and 48% at pH 5.0 and pH 7.0, respectively. The enzyme from Marinomonas sp. SH-2 degraded agarose to neoagarohexaose and neoagarotetraose, indicating that the enzyme is β-agarase. Thus, Marinomonas sp. SH-2 and its enzyme could be practical for applications in food, cosmetic, and medical research.
Agarase;marine bacterium;Marinomonas sp. SH-1;neoagarohexaose;neoagarotetraose;
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