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Simultaneous Analysis Method for Polar and Non-polar Ginsenosides in Cultivated Wild Ginseng by Reversed-phase HPLC-CAD
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 2,  2016, pp.247-252
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.2.247
 Title & Authors
Simultaneous Analysis Method for Polar and Non-polar Ginsenosides in Cultivated Wild Ginseng by Reversed-phase HPLC-CAD
Ok, Seon; Kang, Jae Seon; Kim, Kang Min;
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Cultivated wild ginseng is a widely used dietary supplement and medicinal herb. The aim of this study was to optimize the ginseng using high performance liquid chromatography (HPLC)- charged aerosol detection (CAD) for ginsenoside analysis. CAD measures the physical property of an analyte and responds to almost all non-volatile species, independent of their nature, spectral properties, or particle size. It has become widely employed in pharmaceutical analysis. The cultivated wild ginseng extracts were analyzed for compositions of ginsenosides Rb1, Rd, Rg1, Rf, Re, and Rh1. The optimal analysis condition was set up from an experiment using a gradient. Ten grams of cultivated wild ginseng were extracted with 95% EtOH 100 ml for 24 hr at 80℃. The contents of the 6six major ginsenosides in the cultivated wild ginseng extract were Rb1 (5.48±0.12 mg/g), Rd (5.33±0.14 mg/g), Rg1 (12.80± 0.05 mg/g), Rf (19.08±0.68 mg/g), Re (19.87±0.05 mg/g), and Rh1 (16.47±0.16 mg/g), respectively. HPLC showed that the protopanaxatriol group (Rg1, Rf, Re, Rh1) had more content than the protopanaxadiol group (Rb1, Rd) in cultivated wild ginseng extract. In summary, the ginsenosides were identified with HPLC-CAD analysis, and their presence and quantity imply the importance of quality control, as well as the pharmacological activity of the ginseng root.
Cultivated wild ginseng;charged aerosol detection;gensenoside content;
 Cited by
Testicular antioxidant mechanism of cultivated wild ginseng extracts, Molecular & Cellular Toxicology, 2016, 12, 2, 149  crossref(new windwow)
Ahn, Y. M., Park, H. S. and Kwon, K. R. 2007. Anti-cancer and anti-oxidant efficacies of wild ginseng of korea and china. J. Pharmacopuncture 10, 5-16.

Bombardelli, E, B. A., Gabetta, B. and Martinelli, E. M. 1980. Gas-liquid chromatographic method for determination of ginsenosides in Panax ginseng. J. Chromatogr. A 196, 121-132. crossref(new window)

Choi, K. T. 2008. Botanical characteristics, pharmacological effects and medicinal components of Korean ginseng. Acta. Pharmacol. 29, 1109-1118. crossref(new window)

Choi, J. E., Nam, K. Y., Li, X., Kim, B. Y., Cho, H. S. and Hwang, K. B. 2010. Changes of chemical compositions and ginsenoside contents of different root parts of ginseng with processing method. Kor. J. Medicinal Crop Sci. 18, 118-125.

Christensen, L. P. 2009. Ginsenosides chemistry, biosynthesis, analysis, and potential health effects. Adv. Food Nutr. Res. 55, 1-99.

Chu, C. S., Lee, S. G. and Kwon, K. R. 2004. An experimental study on apoptosis of cultivated wild ginseng distilled herbal acupuncture by controlled pH and electrolyte. J. Kor. Acupunct. Mox. Med. Sci. 21, 1-17.

Corbit, R. M., Ferreira, J. F. S., Ebbs, S. D. and Murphy, L. L. 2005. Simplified extraction of ginsenosides from American ginseng (Panax quinquefolius L.) for high-performance liquid chromatography-ultraviolet analysis. J. Agr. Food Chem. 53, 9867-9873. crossref(new window)

Corthout, J., Naessens, T., Apers, S. and Vlietinck, A. J. 1999. Quantitative determination of ginsenosides from Panax ginseng roots and ginseng preparations by thin layer chromatography-densitometry. J. Pharm. Biomed. Anal. 21, 187-192. crossref(new window)

Jia, L. and Zhao, Y. 2009. Current evaluation of the millennium phytomedicine-ginseng (I): etymology, pharmacognosy, phytochemistry, market and regulations. Curr. Med. Chem. 16, 2475-2484. crossref(new window)

Kim, J. H. and Kim, J. K. 2006. Antioxidant activity and functional component analysis of korean mountain ginseng's different sections. J. Kor. Soc. Food Sci. Nutr. 35, 1315-1321. crossref(new window)

Kim, S. N., Ha, Y. W., Shin, H., Son, S. H., Wu, S. J. and Kim, Y. S. 2007. Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control. J. Pharm. Biomed. Anal. 45, 164-170. crossref(new window)

Kwon, K. S. Lee, C. R., Choi, Y. E., Im, B. O., Sung, J. H. and Yoon, K. R. 2003. Analysis of ginsenosides of white and red ginseng concentrates. Kor. J. Food Sci. Technol. 35, 536-539.

Kwon, H. J., Jeong, J. S., Sim, H. J., Lee, Y. M., Kim, Y. S. and Hong, S. P. 2009. Sensitive high-performance liquid chromatography method of non-polar ginsenosides by alkaline- enhanced pulsed amperometric detection. J. Chromatogr. A 1216, 4445-4450. crossref(new window)

Labonte, P., Kadhim, S., Bowlin, T. and Mounir, S. 2000. Inhibition of tumor growth with doxorubicin in a new orthotopically implanted human hepatocellular carcinoma model. 2003. Hepatol. Res. 18, 72-85. crossref(new window)

Lee, S. H., Kang, J. I. and Lee, S. Y. 2008. Saponin composition and physico-chemical properties of Korean red ginseng extract as affected extracting conditions. J. Kor. Soc. Food Sci. Nutr. 37, 256-260. crossref(new window)

Li, L., Zhang, J. L., Sheng, Y. X., Guo, D. A., Wang, Q. and Guo, H. Z. 2005. Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method. J. Pharm. Biomed. Anal. 38, 45-51. crossref(new window)

Liu, Y., Yang, J. and Cai, Z. 2006. Chemical investigation on Sijunzi decoction and its two major herbs Panax ginseng and Glycyrrhiza uralensis by LC/MS/MS. J. Pharm. Biomed. Anal. 41, 1642-1647. crossref(new window)

Lui, J. H. C. and Staba, E. J. 1980. The ginsenosides of various ginseng plants and selected products. J. Nat. Prod. 43, 340-346. crossref(new window)

Min, B. I., Kim, H. H., Seo, I. B. and Kwon K. R. 2007. Antitumor effects and protective effects against doxorubicin-induced testicular toxicity of cultivated wild ginseng extract in the B16/F10 melanoma-bearing C57BL/6 mice. J. Pharmacopuncture 10, 85-100.

Qu, C., Yang, L., Yu, S., Wang, S., Bai, Y. and Zhang, H. 2009. Investigation of the interactions between ginsenosides and amino acids by mass spectrometry and theoretical chemistry. Spectrochim. Acta A. Mol. Biomol. Spectrosc. 74, 478-483. crossref(new window)

Sievenpiper, J. L., Sung, M. K., DiBuono, M., Seung-Lee, K., Nam, K. Y., Arnason, J. T., Leiter, L. A. and Vuksan, V. 2006. Korean red ginseng rootlets decrease acute postprandial glycemia: results from sequential preparation- and dose-finding studies. J. Am. Coll. Nutr. 25, 100-107. crossref(new window)

The grade quality standards of products of processed ginseng. 1995. Beijing, China. GB/T 15517.1-15517.6.

Tian, Y., Lu, Y., Xie, J., Cheng, Y., Qi, R., Wu, Y. and Zhang, S. 2009. Rapid determination of ginsenoside Rg1, Re and Rb1 in ginseng samples by capillary electrophoresis. Anal. Methods 1, 203-207. crossref(new window)

Wan, J. B., Yang, F. Q., Li, S. P., Wang, Y. T. and Cui, X. M. 2006. Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD. J. Pharm. Biomed. Anal. 41, 1596-1601. crossref(new window)

Wang, A., Wang, C. Z., Wu, J. A., Osinski, J. and Yuan, C. S. 2005. Determination of major ginsenosides in Panax quinquefolius (American ginseng) using high-performance liquid chromatography. Phytochem. Anal. 16, 272-277. crossref(new window)

Wang, C. Z. and Yuan, C. S. 2008. Potential role of ginseng in the treatment of colorectal cancer. Am. J. Chin. Med. 36, 1019-1028. crossref(new window)

Woo, I. H., Yang, C. B. and Sung, H. S. 1986. Effect of different extraction procedures on chemical composition of ginseng extract. Kor. J. Ginseng Sci. 10, 36-44.