Advanced SearchSearch Tips
Anti-metastatic Effect of Garlic Hexane Extract on Lung Metastasis Induced by Melanoma B16F10 Cells in Mice
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 26, Issue 2,  2016, pp.259-264
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.2.259
 Title & Authors
Anti-metastatic Effect of Garlic Hexane Extract on Lung Metastasis Induced by Melanoma B16F10 Cells in Mice
Ko, Min Jung; Rajasekar, Seetharaman; Wang, Ziyu; Li, Mei; Kwak, Jung Ho; Park, Young Hoon; Son, Beung Gu; Kang, Jum Soon; Choi, Young Whan;
  PDF(new window)
Metastatic cancer is one of the main causes of cancer-related death since they rarely respond to available treatments. There is epidemiologic evidence that high garlic consumption decreases the incidence of cancer. Recent studies of our laboratory have revealed that a garlic-extracts is effective in suppressing metastasis. For experimental metastasis, C57BL/6 mice were injected intravenously with melanoma B16F10 cells in the tail vein, and were orally administered various concentrations (0, 50, 100 or 200 mg/kg body weight) of garlic hexane extract (GHE) for 21 days. The incidence and the area of the melanoma cell colony occupied by the poorly differentiated carcinoma were significantly lower in dose-dependent in 50, 100 and 200 mg/kg BW GHE - treated mice compared with control mice. In conclusion, the results of the present study show that GHE administration prevents lung metastasis in C57BL/6 mice.
C57BL/6 mice;garlic hexane extract;melanoma B16F10;metastasis;
 Cited by
Bedrosian, I., Faries, M. B., Guerry, D. t., Elenitsas, R., Schuchter, L., Mick, R., Spitz, F. R., Bucky, L. P., Alavi, A., Elder, D. E., Fraker, D. L. and Czerniecki, B. J. 2000. Incidence of sentinel node metastasis in patients with thin primary melanoma (< or = 1 mm) with vertical growth phase. Ann. Surg. Oncol. 7, 262-267. crossref(new window)

Fidler, I. J. 1973. Selection of successive tumor lines for metastasis. Nature (London) 242, 148-149.

Gautam, A., Waldrep, J. C. and Densmore, C. L. 2000. Inhibition of experimental lung metastasis by aerosol delivery of PEI - p53 complexes. Mol. Ther. 2, 318-323. crossref(new window)

Hanahan, D. and Weinberg, R. A. 2000. The hallmarks of cancer. Cell 100, 57-70. crossref(new window)

Herman-Antosiewicz, A. and Singh, S. V. 2004. Signal transduction pathways leading to cell cycle arrest and apoptosis induction in cancer cells by Allium vegetable-derived organosulfur compounds: a review. Mutat. Res. 555, 121-131. crossref(new window)

Hsing, A. W., Chokkalingam, A. P., Gao, Y. T., Madigan, M. P., Deng, J., Gridley, G. and Fraumeni, J. F. Jr. 2002. Allium vegetables and risk of prostate cancer: a population based study. J. Natl. Cancer Inst. 94, 1648-1651. crossref(new window)

Hu, X., Cao, B. N., Hu, G., He, J., Yang, D. Q. and Wan, Y. S. 2002. Attenuation of cell migration and induction of cell death by aged garlic extract in rat sarcoma cells. Int. J. Mol. Med. 9, 641-643.

Jeong, J. W., Park, S., Park, C., Chang, Y. C., Moon, D. O., Kim, S. O., Kim, G. Y., Cha, H. J., Kim, H. S., Choi, Y. W., Kim, W. J., Yoo, Y. H. and Choi, Y. H. 2014. N-benzyl-N-methyldecan-1-amine, a phenylamine derivative isolated from garlic cloves, induces G2/M phase arrest and apoptosis in U937 human leukemia cells. Oncol. Rep. 32, 373-381.

Kim, E. K., Yun, S. J., Ha, J. M., Jin, I. H., Kim, Y. W., Kim, S. G., Park, D. J., Choi, Y. W., Yoon, S., Kim, C. D. and Bae, S. S. 2011. Inhibition of cancer cell migration by compounds from garlic extracts. J. Life Sci. 21, 767-774. crossref(new window)

Kim, H. J., Han, M. H., Kim, G. Y., Choi, Y. W. and Choi, Y. H. 2012. Hexane extracts of garlic cloves induce apoptosis through the generation of reactive oxygen species in Hep3B human hepatocarcinoma cells. Oncol. Rep. 28, 1757-1763.

Kim, H. K., Choi, Y. W., Lee, E. N., Park, J. K., Kim, S. G., Park, D. J., Kim, B. S., Lim, Y. T. and Yoon, S. 2011. 5-Hydroxymethyl furfural from black garlic extract prevents TNF-α-induced monocytic cell adhesion to HUVECs by suppression of vascular cell adhesion molecule-1 expression, reactive oxygen species generation and NF-κB activation. Phytother. Res. 25, 965-974. crossref(new window)

Kim, K. H., Park, J. K., Choi, Y. W., Kim, Y. H., Lee, E. N., Lee, J. R., Kim, H. S., Baek, S. Y., Kim, B. S., Lee, K. S. and Yoon, S. 2013. Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM-1 in TNF-α-activated human endometrial stromal cells. Int. J. Mol. Med. 32, 67-78.

Lee, S. K., Park, Y. J., Ko, M. J., Wang, Z., Lee, H. Y., Choi, Y. W. and Bae, Y. S. 2015. A novel natural compound from garlic (Alliums ativum L.) with therapeutic effects against experimental polymicrobial epsis. Biochem. Biophys. Res. Commun. 464, 774-779. crossref(new window)

Marsh, C. L., Torrey, R. R., Woolley, J. L., Barker, G. R. and Lau, B. H. 1987. Superiority of intravesical immunotherapy with Corynebacterium parvum and Allium sativum in control of murine bladder cancer. J. Urol. 137, 359-362.

Newman, D. J., Cragg, G. M. and Snader, K. M. 2003. Natural products as sources of new drugs over the period 1981-2002. J. Nat. Prod. 66, 1022-1037. crossref(new window)

Park, H. S., Kim, G. Y., Choi, I. W., Kim, N. D., Hwang, H. J., Choi, Y. W. and Choi, Y. H. 2011. Inhibition of matrix metalloproteinase activities and tightening of tight junctions by diallyldisulfide in AGS human gastric carcinoma cells. J. Food Sci. 76, T105-T111. crossref(new window)

Park, S. Y., Seetharaman, R., Ko, M. J., Kim, D. Y., Kim, T. H., Yoon, M. K., Kwak, J. H., Lee, S. J., Bae, Y. S. and Choi, Y. W. 2014. Ethyl linoleate from garlic attenuates lipopolysaccharide-induced pro-inflammatory cytokine production by inducing heme oxygenase-1 in RAW264.7 cells. Int. Immunopharmacol. 9, 253-261.

Qingjun, C., Ling, M. T., Feng, H., Cheung, H. W., Tsao, S. W., Wang, X. and Wong, Y. C. 2006. A novel anticancer effect of garlic derivatives: inhibition of cancer cell invasion through restoration of E-cadherin expression. Carcinogenesis 27, 2180-2189. crossref(new window)

Riggs, D. R., DeHaven, J. I. and Lamm, D. L. 1997. Allium sativum (garlic) treatment for murine transitional cell carcinoma. Cancer 79, 1987-1994. crossref(new window)

Shin, D. Y., Kim, G. Y., Kim, J. I., Yoon, M. K., Kwon, T. K., Lee, S. J., Choi, Y. W., Kang, H. S., Yoo, Y. H. and Choi, Y. H. 2010. Anti-invasive activity of diallyldisulfide through tightening of tight junctions and inhibition of matrix metalloproteinase activities in LNCaP prostate cancer cells. Toxicol. In Vitro 24, 1569-1576. crossref(new window)

Shin, D. Y., Yoon, M. K., Choi, Y. W., Gweon, O. C., Kim, J. I., Choi T. H. and Choi, Y. H. 2010. Effects of aged black garlic extracts on the tight junction permeability and cell invasion in human gastric cancer cells. J. Life Sci. 20, 528-534. crossref(new window)

Tannock, I. F., de Wit, R., Berry, W. R., Horti, J., Pluzanska, A., Chi, K. N., Oudard, S., Théodore, C., James, N. D., Turesson, I., Rosenthal, M. A. and Eisenberger, M. A. 2004. Docetaxel plus prednisone or mitoxantrone plus prednisone for advanced prostate cancer. N. Engl. J. Med. 351, 1502-1512. crossref(new window)

Teng, M. W., von Scheidt, B., Duret, H., Towne, J. E. and Smyth, M. J. 2011. Anti-IL-23 monoclonal antibody synergizes in combination with targeted therapies or IL-2 to suppress tumor growth and metastases. Cancer Res. 71, 2077-2086. crossref(new window)

Thomson, M. and Ali, M. 2003. Garlic (Allium sativum): a review of its potential use as an anti-cancer agent. Curr. Cancer Drug Targets 3, 67-81. crossref(new window)

Virginia, L. 2006. The analysis of onion and garlic. J. Chroma. A 1112, 3-22. crossref(new window)

William, E. D. Jr., Rosenbaum, L. E. and Bosenberg, M. 2011. Decoding melanoma metastasis. Cancers 3, 126-163.

Yao, Z., Che, X. C., Lu, R., Zheng, M. N., Zhu, Z. F., Li, J. P., Jian, X., Shi, L. X., Liu, J. Y. and Gao, W. Y. 2007. Inhibition by tyroserleutide (YSL) on the invasion and adhesion of the mouse melanoma cell. Mol. Med. 13, 14-21.

Yoon, S. O., Kim, M. M. and Chung, A. S. 2001. Inhibitory effect of selenite on invasion of HT1080 tumor cells. J. Biol. Chem. 276, 20085-20092. crossref(new window)

Yun, H. M., Ban, J. O., Park, K. R., Lee, C. K., Jeong, H. S., Han, S. B. and Hong, J. T. 2014. Potential therapeutic effects of functionally active compounds isolated from garlic. Pharmacol. Ther. 142, 183-195. crossref(new window)