Advanced SearchSearch Tips
Enhancement of Anti-inflammatory Activity by Fermentation of Sargassum siliquanstrum
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 26, Issue 3,  2016, pp.318-324
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.3.318
 Title & Authors
Enhancement of Anti-inflammatory Activity by Fermentation of Sargassum siliquanstrum
Lee, Sol-Ji; Lee, Dong-Geun; Kim, Mihyang; Kong, Chang-Suk; Yu, Ki-Hwan; Kim, Yuck-Young; Lee, Sang-Hyeon;
  PDF(new window)
This study was aimed to verify anti-inflammatory activity of fermented Sargassum siliquanstrum with lactic acid bacteria. Anti-inflammatory activities were compared by measuring the amount of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and suppressive effect on inducible nitric oxide synthase (iNOS) expression in stably transfected RAW 264.7 cells. Inhibitory activities of NO production and iNOS expression were measured after confirmation of NO radical scavenging activities. Fermentation increased NO radical scavenging activities from 7.6% to 15.2% compared to non-fermented condition, and fermentation with Lactobacillus sp. SH-1 was the most efficient. Fermentation without algal debris showed better NO radical scavenging activities than that with debris. Fermentation with Lactobacillus sp. SH-1 also showed the highest NO production inhibitory activity (64.1%) in LPS-stimulated RAW 264.7 cells. LPS-induced iNOS expression was diminished to 28.6, 35.6, 49.4 and 58.5 at 50, 100, 500 and 1,000 μg/ml, respectively, by fermentation with Lactobacillus sp. SH-1. According to MTT assay, fermented S. siliquanstrum did not influence the cell viability at all concentrations tested, meaning no or less cytotoxicity. These results suggest that S. siliquanstrum has NO radical scavenging activity and anti-inflammatory activity. Thus biological activities of S. siliquanstrum were upgraded by fermentation, which could be used for the development of functional foods.
Anti-inflammatory;fermentation;immunity;nitric oxide;Sargassum siliquanstrum;
 Cited by
Effect of seaweed flakes addition on the development of bioactivities in functional Camembert-type cheese, International Journal of Food Science & Technology, 2017, 09505423  crossref(new windwow)
Ahn, S. M., Hong, Y. K., Kwon, G. S. and Sohn, H. Y. 2011. Evaluation of antioxidant and nitrite scavenging activity of seaweed extracts. J. Life Sci. 21, 576-583. crossref(new window)

Andreakos, E., Foxwell, B. and Feldmann, M. 2004. Is targeting Toll-like receptors and their signaling pathway a useful therapeutic approach to modulating cytokine-driven inflammation? Immunol. Rev. 202, 250-265. crossref(new window)

Bredt, D. S. and Snyder, S. H. 1994. Transient nitric oxide synthase neurons in embryonic cerebral cortical plate, sensory ganglia, and olgactory epithelium. Neuron 13, 301-313. crossref(new window)

Chabrier, P. E. and Auguest, M. 1999. Nitric oxide synthases: targets for therapeutic strategies in neurological diseases. Cell Mol. Life Sci. 55, 1029-1035. crossref(new window)

Cho, K. J., Lee, Y. S. and Ryu, B. H. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull. Kor. Fish. Soc. 23, 345-352.

Cho, S. H., Kang, S. E., Cho, J. Y., Kim, A. R., Park, S. M., Hong, Y. K. and Ahn, D. H. 2007. The antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts. J. Med. Food 10, 479-485. crossref(new window)

Chung, M. J., Walker, P. A., Brown, R. W. and Hogstrand, C. 2005. Zinc-mediated gene expression offers protection against H2O2-induced cytotoxicity. Toxicol. Appl. Pharmacol. 205, 225-236. crossref(new window)

Clark, D., Durner, J., Navarre, D. A. and Klessig, D. F. 2000. Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase. MPMI 13, 1380-1384. crossref(new window)

Eom, S. H., Lee, B. J. and Kim, Y. M. 2010. Effect of yeast fermentation on the antioxidant and anti-inflammatory activity of sea tangle water extract. Kor. J. Fish Aquat. Sci. 43, 117-124.

Jaffrey, S. R. and Snyder, S. H. 1995. Nitric oxide: a neural messenger. Annu. Rev. Cell Dev. Biol. 11, 417-440. crossref(new window)

Jeon, M. J., Kim, M. H., Jang, H. J., Lee, S. W., Kim, J. H., Kim, H. S. and Lee, S. H. 2012. Whitening effect of Hizikia fusiformis ethanol extract and its fractions. J. Life Sci. 22, 889-896. crossref(new window)

Kim, A. R. 2008. Antioxidant abilities of extracts from Sargassum siliquanstrum treated with heat, pH and γ-irradiation. M.S. Thesis. Pukyong National University, Busan, Korea.

Kim, C. M. and Park, Y. M. 2009. The effects of different extracts of Ostericum koreanum on the production of imflammatory mediators in LPS-stimulated RAW 264.7 cells. Kor. J. Herbology 24, 169-178.

Kim, E. K. 2012. Studies on the bioactive compounds from Sargassum siliquanstrum. M.S. Thesis. Hanbat National University, Daejeon, Korea.

Kim, J. Y., Kim, K. H., Suh, H. S. and Choi, W. C. 1997. Antiinflammatory effects of new chemical compounds, HS-1580 series(HS-1580, HS-1581, HS-1582). J. Life Sci. 16, 1181-1187.

Kim, M. J. and Kim, G. R. 2006. In vitro evaluation of cholesterol reduction by lactic acid bacteria extracted from kimchi. Kor. J. Culinary Res. 12, 259-268.

Kim, M. J., Song, E. J., Lee, S. Y., Kim, K. B. W. R., Kim, S. J., Lee, S. J., Yoon, S. Y., Kim, A. R., Jeon, Y. J., Park, J. G., Choi, J. I., Lee, J. W., Byun, M. W. and Ahn, D. H. 2008. Effects of γ-irradiation on antioxidant and physicochemical properties of Ishige okamurai extracts. J. Kor. Soc. Food Sci. Nutr. 37, 1485-1490. crossref(new window)

Kim, M. K., Kim, M. Y., Youn, E. K. and Kim, S. D. 2002. Extraction of citrus bioflavonoid with vinegars and effect on blood pressure. Kor. J. Food Preserv. 9, 411-417.

Kim, N. Y., Jang, H. J., Lee, D. G., Jang, M. K., Lee, S. W., Jeon, M. J., Kim, M. H., Kim, S. G. and Lee, S. H. 2011. Establishment of in vitro detection system for iNOS expression and the verification of suppressive effect by pine needle extract. KSBB J. 26, 172-176. crossref(new window)

Kwon, M. S., Mun, O. J., Bae, M. J., Lee, S. G., Kim, M. H., Lee, S. H., Yu, K. H., Kim, Y. Y. and Kong, C. S. 2015. Anti-inflammatory activity of ethanol extracts from Hizikia fusiformis fermented with lactic acid bacteria in LPS-stimulated RAW 264.7 macrophages. J. Kor. Soc. Food Sci. Nutr. 44, 1450-1457. crossref(new window)

Lee, H. J., Kim, J. H., Lee, C. H., Kim, J. S., Kwak, S. T., Lee, K. B., Song, K. S., Choi, B. W. and Lee, B. H. 1999. Inhibition activities of sea weeds on prolyl endopeptidase, tyrosinase and coagulation. Kor. J. Pharmacogn. 30, 231-237.

Lee, S. J., Lee, D. G., Park, S. H., Kim, M. H., Kong, C. S., Kim, Y. Y. and Lee, S. H. 2015. Comparison of biological activities in Sargassum siliquanstrum fermented by isolated lactic acid bacteria. Biotechnol. Bioprocess. Eng. 20, 341-348. crossref(new window)

Lee, S. Y., Kim, J. H., Kim, K. B. W. R., Song, E. J., Kim, A. R., Park, S. M., Han, C. S. and Ahn, D. H. 2007. Antimicrobial activities of medicinal herbs and seaweeds extracts against microorganisms isolated from the rice warehouses. J. Kor. Soc. Food Sci. Nutr. 36, 476-480. crossref(new window)

Lee, S. Y., Kim, K. B. W. R., Song, E. J., Kim, J. H., Kim, A. R., Kim, M. J., Moon, J. H., Kang, H. M., Lee, H. D., Hong, Y. K. and Ahn, D. H. 2008. Effect of extracts from Sargassum siliquanstrum on shelf-life and quality of bread. J. Kor. Soc. Food Sci. Nutr. 37, 490-496. crossref(new window)

Liu, J. N., Yoshida, Y., Wang, M. Q., Okai, Y. and Yamashita, U. 1997. B cell stimulating activity of seaweed extracts. Int. J. Immunopharmacol. 19, 135-142.

Marcocci, L., Packer, L., Dory-Lefaix, M. T., Sakaki, A. and Gardes-Albert, M. 1994. Antioxidant action of Ginkgo biloba extract Egb 76. Methods Enzymol. 234, 462-475. crossref(new window)

Moncada, S. 1999. Nitric oxide: discovery and impact on clinical medicine. J. R. Soc. Med. 92, 164-169. crossref(new window)

Numata, A., Kanbara, S., Takahashi, C., Fujiki, R., Yoneda, M., Usami, Y. and Fujita, E. 1992. A cytotoxic principle of the brown alga Sargassum tortile and structures of chromenes. Phytochemistry 31, 1209-1213. crossref(new window)

Park, J. C., Choi, J. S., Song, S. H., Choi, M. R., Kim, K. Y. and Choi, J. W. 1997. Hapatoprotective effect of extracts and phenolic compound from marine algae in bromobenzene-treated rats. Kor. J. Pharmacogn. 28, 239-246.

Park, M. J. and Han, J. S. 2006. Radical scavenging and anti-oxidant activities of fermented Laminaria japonica extracts. J. Food Sci. Nutr. 11, 10-16. crossref(new window)

Park, S. H., Kim, J. I., Jeong, Y. K. and Choi, Y. H. 2011. Extracts of lipopolysaccharide-stimulated BV2 microgia cells. J. Life Sci. 21, 796-804. crossref(new window)

Schwartsmann, G., Rocha, A. B., Berlinck, G. S. and Jimeno, J. 2001. Marine organisms as a source of new anticancer agents. Lancet Oncol. 2, 221-225. crossref(new window)

Shobharani, P., Halani, P. M. and Sachindra, N. M. 2012. Potential of marine lactic acid bacteria to ferment Sargassum sp. for enhances anticoagulant and antioxidant properties. J. Appl. Microbiol. 114, 96-107.

Song, H. S., Kim, H. K., Min, H. O., Choi, J. D. and Kim, Y. M. 2011. Change of physicochemical and sensory properties of Hizikia fusiformis water extract by the fermentation of lactic acid bacteria. Kor. J. Fish Aquat. Sci. 44, 104-110.

Yang, J. O., Yoo, C. J., Kim, J. O. and Che, M. E. 1999. Utilization fermented tea-fungus beverage for the sports drink. Kor. J. Phys. Edu. 38, 277-293.

Yoon, T. J., Yoo, Y. C., Kang, T. B., Lee, K. H., Kwak, J. H., Baek, Y. J., Huh, C. S. and Kim, J. B. 1999. Fermented extracts of Korean mistletoe with Lactobacillus (FKM-110) stimulate macrophage and inhibit tumor metastasis. Kor. J. Food Sci. Technol. 31, 838-847.