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Rosuvastatin Induces ROS-mediated Apoptosis in Human Prostate Cancer PC-3 Cells
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 4,  2016, pp.398-405
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.4.398
 Title & Authors
Rosuvastatin Induces ROS-mediated Apoptosis in Human Prostate Cancer PC-3 Cells
Choi, Hyeun Deok; Baik, Jong Jin; Kim, Sang Hun; Yu, Sun Nyoung; Chun, Sung Hak; Kim, Young Wook; Nam, Hyo Won; Kim, Kwang Youn; Ahn, Soon Cheol;
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Statins, the inhibitors of 3-hydroxy 3-methylglutaryl coenzyme A (HMG-CoA) reductase, are widely used in treatments of hypercholesterolemia and newly known as anti-cancer effect of various cancer cells. Recently, several studies suggested that reactive oxygen species (ROS) play a critical role on cell death signaling. However, mechanism of ROS by rosuvastatin is currently unclear. This study aimed to explore the molecular mechanism of apoptosis by rosuvastatin in human prostate cancer PC-3 cells. Cell viability and apoptosis-related protein expression were measured by MTT assay and western blotting, respectively. In addition, the levels of apoptosis and ROS were analyzed. The results showed that rosuvastatin dramatically reduced cell viability in a dose- and time-dependent manner. We confirmed that rosuvastatin induced apoptosis through reduction of procaspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) in PC-3 cells. In addition, rosuvastatin stimulated ROS production in a dose-dependent manner and pre-treatment with N-acetylcysteine (NAC), a ROS scavenger, significantly recovered rosuvastatin-induced ROS and apoptosis. Thus, we concluded that rosuvastain induces apoptosis through generation of ROS in human prostate cancer PC-3 cells and provides a promising approach to improve the efficacy of cancer therapy.
Apoptosis;human prostate cancer PC-3 cells;N-acetylcysteine;reactive oxygen species;rosuvastatin;
 Cited by
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