JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Phylogenetic Study of Genus Haliotis in Korea by Cytochrome c Oxidase Subunit 1 and RAPD Analysis
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 26, Issue 4,  2016, pp.406-413
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.4.406
 Title & Authors
Phylogenetic Study of Genus Haliotis in Korea by Cytochrome c Oxidase Subunit 1 and RAPD Analysis
Seo, Yong Bae; Kang, Sung Chul; Choi, Seong Seok; Lee, Jong Kyu; Jeong, Tae Hyug; Lim, Han Kyu; Kim, Gun-Do;
  PDF(new window)
 Abstract
Abalones are gastropod mollusks belonging to the genus Haliotis. Pacific abalones are regarded as a very important marine gastropod mollusk in Korea, Japan, China, and also in food industries around the world. In Korea, 6 species of abalone have been reported to occur along the coasts: Haliotis discus hannai, Haliotis discus discus, Haliotis madaka, Haliotis gigantea, Haliotis diversicolor supertexta, and Haliotis diversicolor diversicolor. This study was performed to discriminate the genetic variances by the partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) genes and random amplified polymorphic DNA (RAPD) analysis against four species of Pacific abalone (H. discus hannai, H. discus, H. madaka, H. gigantea). COI gene is reasonably well conserved and has been sequenced in various invertebrate taxa. The RAPD analysis technique is a relatively simple and low cost method that allows differentiation of taxa without the need to know their genomes. In this study, we investigated the genetic diversity, phylogenetic relationships within each species. The COI and RAPD analysis were able to distinguish between H. gigantea and the other three species. However, these analysis methods were inadequate to distinguish between H. discus and H. madaka. These results are believed to be able to provide a basis data for future hybrid breeding research by defining the genetically closely related four species of abalone, which is to develop new hybrid abalone for export using hybrid breeding.
 Keywords
COI (cytochrome c oxidase subunit I);Haliotis discus;Haliotis gigantea;Haliotis madaka;RAPD (random amplified polymorphic DNA);
 Language
Korean
 Cited by
 References
1.
Ali, B. A., Huang, T. H., Qin, D. N. and Wang, X. M. 2004. A review of random amplified polymorphic DNA (RAPD) markers in fish research. Rev. Fish Biol. Fish. 14, 443-453. crossref(new window)

2.
An, H. S., Jee, Y. J., Min, K. S., Kim, B. L. and Han, S. J. 2005. Phylogenetic analysis of six species of Pacific abalone (Haliotidae) based on DNA sequences of 16s rRNA and cytochrome c oxidase subunit I mitochondrial genes. Mar. Biotechnol. 7, 373-380. crossref(new window)

3.
Asahida, T., Kobayashi, T., Saitoh, K. and Nakayarma, I. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing of urea. Fish. Sci. 62, 727-730. crossref(new window)

4.
Bardakci, F. and Skibinski, D. O. 1994. Application of the RAPD technique in tilapia fish: species and subspecies identification. Heredity 73, 117-123. crossref(new window)

5.
Birky, C. W., Fuerst, P. and Maruyamat, T. 1989. Organelle gene diversity under migration, mutation, and drift: equilibrium expectations, approach to equilibrium, effects of heteroplasmic cells, and comparison to nuclear genes. Genetics 121, 613-627.

6.
Doherty, K. R., Zweifel, E. W., Elde, N. C., McKone, M. J. and Zweifel, S. G. 2003. Random amplified polymorphic DNA markers reveal genetic variation in the symbiotic fungus of leaf-cutting ants. Mycologia 95, 19-23. crossref(new window)

7.
Geiger, D. L. 1998. Recent genera and species of the family Haliotidae Rafinesque, 1815 (Gastropoda, Vetigastyropoda). Nautilus 111, 85-116.

8.
Hamm, D. E. and Burton, R. S. 2000. Population genetics of black abalone, Haliotis cracherodii, along the central California coast. J. Exp. Mar. Bio. Ecol. 254, 235-247. crossref(new window)

9.
Hebert, P. D., Ratnasingham, S. and deWaard, J. R. 2003. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc. Biol. Sci. 270, 96-99. crossref(new window)

10.
Jones, C. J., Edwards, K. J., Castaglione, S., Winfield, M. O., Sala F., van deWiel, C., Bredemeijer, G., Vosman, B., Matthes, M., Daly, A., Brettschneider, R., Bettini, P., Buiatti, M., Maestri, E., Malcevschi, A., Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. and Karp, A. 1997. Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Mol. Breed. 3, 381-390. crossref(new window)

11.
Metz, E. C., Robles-Sikisaka, R. and Vacquier, V. D. 1998. Nonsynonymous substitution in abalone sperm fertilization genes exceeds substitution in introns and mitochondrial DNA. Proc. Natl. Acad. Sci. USA 95, 10676-10681. crossref(new window)

12.
Park, C. J., Kim, H. C., Noh, J. K., Lee, J. H. and Myeong, J. I. 2008. Genetic divergence and relationship among abalone species by RAPD analysis. J. Aquaculture 21, 346-350.

13.
Sesli, M. and Yegenoglu, E. D. 2010. Comparison of similarity coefficients used for cluster analysis based on RAPD markers in wild olives. Genet. Mol. Res. 9, 2248-53. crossref(new window)

14.
Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535. crossref(new window)

15.
Won, S. H., Kim, S. K., Kim, S. C., Yang, B. K., Lim, B. S., Lee, J. H., Lim, H. K., Lee, J. S. and Lee, J. S. 2014. The Morphological characteristics of four Korean Abalone species in Nordotis. Kor. J. Malacol. 30, 87-93. crossref(new window)