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New Four-herb Formula Ameliorates Memory Impairments via Neuroprotective Effects on Hippocampal Cells
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 4,  2016, pp.475-483
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.4.475
 Title & Authors
New Four-herb Formula Ameliorates Memory Impairments via Neuroprotective Effects on Hippocampal Cells
Ahn, Sung Min; Choi, Young Whan; Shin, Hwa Kyoung; Choi, Byung Tae;
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 Abstract
The current study was conducted to evaluate beneficial effects of a new formula (CWC-9) using four traditional Oriental medicinal herbs, Cynanchum wilfordii, Rehmannia glutinosa, Polygala tenuifolia, and Acorus gramineus, on hippocampal cells and memory function. To examine the neuroprotective effects of a new four-herb extract, cell viability, cytotoxicity, and reactive oxygen species (ROS) assays were performed in HT22 cells and behavioral tests (Morris water maze and passive avoidance retention), Western blot, and immunohistochemistry were performed in a mouse model of focal cerebral ischemia. In HT22 hippocampal cells, pretreatment with CWC-9 resulted in significantly reduced glutamate-induced cell death with suppression of ROS accumulation caused by glutamate. In a mouse model of focal cerebral ischemia, we observed significant improvement of spatial and short-term memory function by treatment with CWC-9. Phosphorylated p38 mitogen-activated protein kinases (MAPK) in hippocampus of ischemic mice were decreased by treatment with CWC-9, but phosphorylated phosphatidylinositol-3 kinase (PI3K) and cAMP response element binding protein (CREB) were significantly enhanced. By immunohistochemical analysis, we confirmed higher expression of phosphorylation of CREB in the hippocampal neurons of CWC-9 treated mice. These results suggest that new multi-herb formula CWC-9 mainly exerted beneficial effects on cognitive function through regulation of neuro-protective signaling pathways associated with CREB.
 Keywords
Focal cerebral ischemia;hippocampal neurons;memory;neuroprotection;
 Language
English
 Cited by
 References
1.
Anderson, C. N. and Tolkovsky, A. M. 1999. A role for MAPK/ERK in sympathetic neuron survival: protection against a p53-dependent, JNK-independent induction of apoptosis by cytosine arabinoside. J. Neurosci. 19, 664-673.

2.
Bonni, A., Brunet, A., West, A. E., Datta, S. R., Takasu, M. A. and Greenberg, M. E. 1999. Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science 286, 1358-1362. crossref(new window)

3.
Cechetti, F., Worm, P. V., Elsner, V. R., Bertoldi, K., Sanches, E., Ben, J., Siqueira, I. R. and Netto, C. A. 2012. Forced treadmill exercise prevents oxidative stress and memory deficits following chronic cerebral hypoperfusion in the rat. Neurobiol. Learn. Mem. 97, 90-96. crossref(new window)

4.
Collino, M., Aragno, M., Mastrocola, R., Gallicchio, M., Rosa, A. C., Dianzani, C., Danni, O., Thiemermann, C. and Fantozzi, R. 2006. Modulation of the oxidative stress and inflammatory response by PPAR-gamma agonists in the hippocampus of rats exposed to cerebral ischemia/reperfusion. Eur. J. Pharmacol. 530, 70-80. crossref(new window)

5.
Coyle, J. T. and Puttfarcken, P. 1993. Oxidative stress, glutamate, and neurodegenerative disorders. Science 262, 689-695. crossref(new window)

6.
Debette, S. 2013. Vascular risk factors and cognitive disorders. Rev. Neurol. (Paris) 169, 757-764. crossref(new window)

7.
Dineley, K. T., Westerman, M., Bui, D., Bell, K., Ashe, K. H. and Sweatt, J. D. 2001. Beta-amyloid activates the mitogen-activated protein kinase cascade via hippocampal alpha7 nicotinic acetylcholine receptors: In vitro and in vivo mechanisms related to Alzheimer′s disease. J. Neurosci. 21, 4125-4133.

8.
Fukui, M., Choi, H. J. and Zhu, B. T. 2010. Mechanism for the protective effect of resveratrol against oxidative stress-induced neuronal death. Free Radic. Biol. Med. 49, 800-813. crossref(new window)

9.
Ghosh, A., Sarkar, S., Mandal, A. K. and Das, N. 2013. Neuroprotective role of nanoencapsulated quercetin in combating ischemia-reperfusion induced neuronal damage in young and aged rats. PLoS One 8, e57735.

10.
Gim, S. A. and Koh, P. O. 2014. Ferulic acid prevents the injury-induced decrease of gamma-enolase expression in brain tissue and HT22 cells. Lab. Animal Res. 30, 8-13. crossref(new window)

11.
Hwang, B. Y., Kim, Y. H., Ro, J. S., Lee, K. S. and Lee, J. J. 1999. Acetophenones from the roots of Cynanchum wilfordii H(EMSLEY). Arch. Pharm. Res. 22, 72-74. crossref(new window)

12.
Itua, I. and Naderali, E. K. 2010. Review: omega-3 and memory function: to eat or not to eat. Am. J. Alzheimers Dis. Other. Demen. 25, 479-482. crossref(new window)

13.
Jamarkattel-Pandit, N., Pandit, N. R., Kim, M. Y., Park, S. H., Kim, K. S., Choi, H., Kim, H. and Bu, Y. 2010. Neuroprotective effect of defatted sesame seeds extract against in vitro and in vivo ischemic neuronal damage. Planta Med. 76, 20-26. crossref(new window)

14.
Kitagawa, K. 2007. CREB and cAMP response elementmediated gene expression in the ischemic brain. FEBS J. 274, 3210-3217. crossref(new window)

15.
Lasa, M., Abraham, S. M., Boucheron, C., Saklatvala, J. and Clark, A. R. 2002. Dexamethasone causes sustained expression of mitogen-activated protein kinase (MAPK) phosphatase 1 and phosphatase-mediated inhibition of MAPK p38. Mol. Cell. Biol. 22, 7802-7811. crossref(new window)

16.
Lee, K. Y., Jeong, E. J., Huh, J., Cho, N., Kim, T. B., Jeon, B. J., Kim, S. H., Kim, H. P. and Sung, S. H. 2012. Cognition-enhancing and neuroprotective activities of the standardized extract of Betula platyphylla bark and its major diarylheptanoids. Phytomedicine 19, 1315-1320. crossref(new window)

17.
Lee, M. K., Yeo, H., Kim, J., Markelonis, G. J., Oh, T. H. and Kim, Y. C. 2000. Cynandione A from Cynanchum wilfordii protects cultured cortical neurons from toxicity induced by H2O2, L-glutamate, and kainate. J. Neurosci. Res. 59, 259-264. crossref(new window)

18.
Lo, E. H., Dalkara, T. and Moskowitz, M. A. 2003. Mechanisms, challenges and opportunities in stroke. Nat. Rev. Neurosci. 4, 399-415.

19.
Maher, P. and Davis, J. B. 1996. The role of monoamine metabolism in oxidative glutamate toxicity. J. Neurosci. 16, 6394-6401.

20.
May, B. H., Lu, C., Bennett, L., Hugel, H. M. and Xue, C. C. 2012. Evaluating the traditional Chinese literature for herbal formulae and individual herbs used for age-related dementia and memory impairment. Biogerontology 13, 299-312. crossref(new window)

21.
Qin, R., Li, X., Li, G., Tao, L., Li, Y., Sun, J., Kang, X. and Chen, J. 2011. Protection by tetrahydroxystilbene glucoside against neurotoxicity induced by MPP+: the involvement of PI3K/Akt pathway activation. Toxicol Lett. 202, 1-7. crossref(new window)

22.
Stanciu, M., Wang, Y., Kentor, R., Burke, N., Watkins, S., Kress, G., Reynolds, I., Klann, E., Angiolieri, M. R., Johnson, J. W. and DeFranco, D. B. 2000. Persistent activation of ERK contributes to glutamate-induced oxidative toxicity in a neuronal cell line and primary cortical neuron cultures. J. Biol. Chem. 275, 12200-12206. crossref(new window)

23.
Tan, S., Sagara, Y., Liu, Y., Maher, P. and Schubert, D. 1998. The regulation of reactive oxygen species production during programmed cell death. J. Cell Biol. 141, 1423-1432. crossref(new window)

24.
Tan, S., Schubert, D. and Maher, P. 2001. Oxytosis: A novel form of programmed cell death. Curr. Top. Med. Chem. 1, 497-506. crossref(new window)

25.
Taylor, C. P., Weber, M. L., Gaughan, C. L., Lehning, E. J. and LoPachin, R. M. 1999. Oxygen/glucose deprivation in hippocampal slices: altered intraneuronal elemental composition predicts structural and functional damage. J. Neurosci. 19, 619-629.

26.
Xu, J., Xilouri, M., Bruban, J., Shioi, J., Shao, Z., Papazoglou, I., Vekrellis, K. and Robakis, N. K. 2011. Extracellular progranulin protects cortical neurons from toxic insults by activating survival signaling. Neurobiol. Aging 32, 2326.

27.
Yamamoto-Sasaki, M., Ozawa, H., Saito, T., Rosler, M. and Riederer, P. 1999. Impaired phosphorylation of cyclic AMP response element binding protein in the hippocampus of dementia of the Alzheimer type. Brain Res. 824, 300-303. crossref(new window)

28.
Yue, R., Yuan, X., Liu, X., Zhang, J., Jiang, P., He, C., Shan, L., Yu, Y. and Zhang, W. 2012. Cynandione A mitigates ischemic injuries in rats with cerebral ischemia. J. Neurochem. 121, 451-464. crossref(new window)