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A Recombinant Microbial Biosensor for Cadmium and Lead Detection
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 5,  2016, pp.503-508
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.5.503
 Title & Authors
A Recombinant Microbial Biosensor for Cadmium and Lead Detection
Shin, Hae Ja;
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Biosensors have been used as first-step monitoring tools to detect on-site samples in a simple and cost-effective manner. Numerous recombinant microbial biosensors have been exploited for monitoring on-site toxic chemicals and biological signals. Herein, a recombinant microbial biosensor was constructed for monitoring cadmium. The cadmium responding cadC regulatory gene and it’s promoter from Staphylococcus aureus was amplified through PCR, fused with the lacZ gene, and transformed into Escherichia coli BL21 (DE3) cells. In the presence of cadmium, the biosensor cells express β-galactosidase showing red color development with chlorophenol red β-galactopyranoside (CPRG) as the enzymatic substrate. The biosensor cells showed the best β-galactosidase activity after 3 hr induction with cadmium at pH 5 and a detection range from 0.01 μM to 10 mM cadmium with a linearity from 0.01 to 0.1 μM cadmium (y
Cadmium;heavy metals;recombinant microbial biosensor;
 Cited by
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