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Anti-oxidant and Anti-inflammatory Activities of Barley Sprout Extract
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 5,  2016, pp.537-544
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.5.537
 Title & Authors
Anti-oxidant and Anti-inflammatory Activities of Barley Sprout Extract
Eun, Cheong-Su; Hwang, Eun-Young; Lee, Syng-Ook; Yang, Seun-Ah; Yu, Mi-Hee;
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Barley (Hardeum vulgare L.) sprout has received much attention in recent years as a functional food in many countries, especially in Korea and Japan. It has been reported that barley sprouts are comprised of 52.6% polysaccharides, 34.1% proteins, and 4.97% fats, along with a variety of vitamins, minerals, and polyphenols. The purpose of this study was to assess the anti-oxidant and anti-inflammatory activities of the ethanol extracts of barley sprouts. We examined the inhibitory effect of barley sprout extracts (BSE) on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, and nitric oxide (NO), prostaglandin E2 (PGE2) and cytokine production in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophage cells. BSE contains high amounts of phenolics and flavonoids and exhibits potent anti-oxidative activity, as depicted by the DPPH radical-scavenging experiment. The concentration of total phenols was 17.55 μg/ml, and flavonoids, 13.98 μg/ml. We also investigated the anti-inflammatory activities of BSE in LPS-stimulated RAW 264.7 cells. Tumor necrosis factor-alpha and PGE2 production, which had increased as a result of treatment with LPS, were significantly inhibited by BSE in a dose-dependent manner. BSE also significantly suppressed LPS-induced production of NO, and this was accompanied by a decrease in the expression of the iNOS and COX-2 proteins. These results indicate that barley sprouts may be a highly valuable natural product owing to its high-quality functional components as well as its anti-oxidant and anti-inflammatory activities.
Anti-inflammatory;anti-oxidant;barley sprout;macrophage;Raw 264.7;
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