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Influence of Chromosome Number on Cell Growth and Cell Aging in Yeast
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 6,  2016, pp.646-650
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.6.646
 Title & Authors
Influence of Chromosome Number on Cell Growth and Cell Aging in Yeast
Kim, Yeon-Hee;
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The influence of chromosome number on cell growth and cell aging was investigated in various yeast strains that have many artificial chromosomes constructed using a chromosome manipulation technique. Host strain FY833 and the YKY18, YKY18R, YKY24, and YKY30 strains harboring 16 natural chromosomes, 18 chromosomes, 18 chromosomes containing rDNA chromosome, 24 chromosomes, and 30 chromosomes, respectively, were used, and the specific growth rate of each strain was compared. The specific growth rates in the YKY18 and YKY24 strains were indistinguishable from that in the host strain, while those of the YKY18R and YKY30 strains were reduced to approximately 25% and 40% of the host strain level, respectively. Subsequently, the replicative life span was examined to investigate the relationship between the number of chromosomes and cell aging, and the life span was decreased to approximately 14% and 45% of the host strain level in the YKY24 and YKY30 strains, respectively. Moreover, telomere length, well known as a senescence factor, was shorter and more diversified in the strain, showing decreased life span. Therefore, these results suggest the possibility that an increase in the number of chromosomes containing artificial chromosomes caused cell aging, and we expected these observations would be applied to improve industrial strain harboring of versatile and special artificial chromosomes.
Artificial chromosome;cell growth rate;life span;telomere length;Saccharomyces cerevisiae;
 Cited by
Banerjee, S. and Myung, K. 2004. Increased genome instability and telomere length in the elg1-deficient Saccharomyces cerevisiae mutant are regulated by S-phase check-points. Eukaryot. Cell 3, 1557-1566. crossref(new window)

Bitterman, K. J., Medvedik, O. and Sinclair, D. A. 2003. Longevity regulation in Saccharomyces cerevisiae: Linking metabolism, genome stability, and heterochromatin. Microbiol. Mol. Biol. Rev. 67, 376-399. crossref(new window)

Burke, D., Dawson, D. and Stearns, T. 2000. Methods in yeast genetics, pp. 110-111, A Cold Spring Harbor Laboratory Course Manual. A Cold Spring Harbor Laboratory, Cold Spring Harbor. New York.

Burke, D. T., Carle, G. F. and Olson, M. V. 1987. Cloning of large segments of DNA into yeast by means of artificial chromosome vectors. Science 236, 806-812. crossref(new window)

Egilmez, N. K. and Jazwinski, S. M. 1989. Evidence for the involvement of a cytoplasmic factor in the aging of the yeast Saccharomyces cerevisiae. J. Bacteriol. 1, 37-42.

Gillespie, C. S., Proctor, C. J., Boys, R. J., Shanley, D. P., Wilkinson, D. J. and Kirkwood, T. B. 2004. A mathematical model of ageing in yeast. J. Theor. Biol. 229, 189-196. crossref(new window)

Jazwinski, S. M. 1999. Longevity, genes, and aging: A view provided by a genetic model system. Exp. Gerontol. 34, 1-6. crossref(new window)

Kaeberlein, M. 2006. Longevity and aging in the budding yeast. In: Conn PM, editor. Handbook of models for human aging. Boston: Elvesier Press pp. 109-120.

Kennedy, B. K., Austriaco, N. R. Jr. and Guarente, L. 1994. Daughter cells of Saccharomyces cerevisiae from old mothers display a reduced life span. J. Cell Biol. 127, 1985-1993. crossref(new window)

Kim, Y. H., Ishikawa, D., Ha, H. P., Sugiyama, M., Kaneko, Y. and Harashima, S. 2006. Chromosome XII context is important for rDNA function in yeast. Nucleic Acids Res. 34, 2914-2924. crossref(new window)

Louis, E. J. and Haber, J. E. 1992. The structure and evolution of subtelomeric Y’ repeats in Saccharomyces cerevisiae. Genetics 131, 559-574.

Masoro, E. J. 2005. Overview of caloric restriction and ageing. Mech. Ageing Dev. 126, 913-922. crossref(new window)

Melov, S., Ravenscroft, J., Malik, S., Gill, M. S., Walker, D. W., Clayton, P. E., Wallace, D. C., Malfroy, B., Doctrow, S. R. and Lithgow, G. J. 2000. Extension of life-span with superoxide dismutase/catalase mimetics. Science 289, 1567-1569. crossref(new window)

Mortimer, R. K. and Johnston, J. R. 1959. Life span of individual yeast cells. Nature 183, 1751-1752. crossref(new window)

Park, A. H. and Kim, Y. H. 2013. Breeding of ethanol producing and tolerant Saccharomyces cerevisiae by using genome shuffling. J. Life Sci. 23, 1192-1198. crossref(new window)

Park, A. H., Sugiyama, M., Harashima, S. and Kim, Y. H. 2012. Creation of an ethanol-tolerant yeast strain by genome reconstruction based on chromosome splitting technology. J. Microbiol. Biotechnol. 22, 184-189. crossref(new window)

Piper, P. W. 2006. Long-lived yeast as a model for ageing research. Yeast 23, 215-226. crossref(new window)

Sinclair, D. A. and Guarente, L. 1997. Extrachromosomal rDNA circles-A cause of aging in yeast. Cell 91, 1033-1042. crossref(new window)

Sugiyama, M., Ikushima, S., Nakazawa, T., Kaneko, Y. and Harashima, S. 2005. PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae. Biotechniques 38, 909-914. crossref(new window)

Winston, F., Dollard, C. and Ricupero-Hovasse, S. L. 1995. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11, 53-55. crossref(new window)