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Cloning of the Bombyx mori short neuropeptide F receptor (BsNPF-R) cDNA
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  • Journal title : Journal of Life Science
  • Volume 26, Issue 6,  2016, pp.721-726
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.6.721
 Title & Authors
Cloning of the Bombyx mori short neuropeptide F receptor (BsNPF-R) cDNA
Shin, Hyojung; Kwon, Kisang; Hong, Sun Mee; Kim, Hong Geun; Park, Kwan-Ho; Choi, Ji-Young; Kim, Seung-Whan; Yu, Kweon; Kwon, O-Yu;
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 Abstract
It has already been reported that short neuropeptide F (sNPF) stimulates feeding behaviors in a wide variety of insect species. In the present study, we cloned cDNA, encoding a sNPF receptor homologue from a silkworm, Bombyx mori, named BsNPF-R. The amino acid sequence of BsNPF-R was compared with those of sNPF-R thus far reported, which is shared with humans (36%), mice (34%), zebrafish (35%), and fruit flies (51%), respectively. A BsNPF-R protein’s mass was theoretically estimated to be 42,731 Da and it is a putative plasma membrane-penetrating protein. The mRNA expression of BsNPF-R was tested; the results showed that a strong expression was detected at the midgut, post-silk gland, Malpighian, and testis; however, a weak expression was at the fat body, hemocyte, and ovary. In addition, the synthesized sNPF of a silkworm regulated the BsNPF-R mRNA expression through the cell-based functional analysis.
 Keywords
Bombyx mori;B. mori short neuropeptide F receptor (BsNPF-R);cDNA;
 Language
Korean
 Cited by
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