Advanced SearchSearch Tips
Cloning of the Bombyx mori short neuropeptide F receptor (BsNPF-R) cDNA
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 26, Issue 6,  2016, pp.721-726
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2016.26.6.721
 Title & Authors
Cloning of the Bombyx mori short neuropeptide F receptor (BsNPF-R) cDNA
Shin, Hyojung; Kwon, Kisang; Hong, Sun Mee; Kim, Hong Geun; Park, Kwan-Ho; Choi, Ji-Young; Kim, Seung-Whan; Yu, Kweon; Kwon, O-Yu;
  PDF(new window)
It has already been reported that short neuropeptide F (sNPF) stimulates feeding behaviors in a wide variety of insect species. In the present study, we cloned cDNA, encoding a sNPF receptor homologue from a silkworm, Bombyx mori, named BsNPF-R. The amino acid sequence of BsNPF-R was compared with those of sNPF-R thus far reported, which is shared with humans (36%), mice (34%), zebrafish (35%), and fruit flies (51%), respectively. A BsNPF-R protein’s mass was theoretically estimated to be 42,731 Da and it is a putative plasma membrane-penetrating protein. The mRNA expression of BsNPF-R was tested; the results showed that a strong expression was detected at the midgut, post-silk gland, Malpighian, and testis; however, a weak expression was at the fat body, hemocyte, and ovary. In addition, the synthesized sNPF of a silkworm regulated the BsNPF-R mRNA expression through the cell-based functional analysis.
Bombyx mori;B. mori short neuropeptide F receptor (BsNPF-R);cDNA;
 Cited by
Bajracharya, P., Lu, H. L. and Pietrantonio, P. V. 2014. The red imported fire ant (Solenopsis invicta Buren) kept Y not F: predicted sNPY endogenous ligands deorphanize the short NPF (sNPF) receptor. PLoS One 9, e109590. crossref(new window)

Beck-Sickinger, A. G. and Jung, G. 1995. Structure-activity relationships of neuropeptide Y analogues with respect to Y1 and Y2 receptors. Biopolymers 37, 123-142. crossref(new window)

Bigot, L., Beets, I., Dubos, M. P., Boudry, P., Schoofs, L. and Favrel, P. 2014. Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mol lusk Crassostrea gigas. J. Exp. Biol. 217, 2974-2982. crossref(new window)

Dillen, S., Verdonck, R., Zels, S., Van Wielendaele, P. and Vanden Broeck, J. 2014. Identification of the short neuropeptide F precursor in the desert locust: evidence for an inhibitory role of sNPF in the control of feeding. Peptides 53, 134-139. crossref(new window)

Garczynski, S. F., Crim, J. W. and Brown, M. R. 2007. Characterization and expression of the short neuropeptide F receptor in the African malaria mosquito, Anopheles gambiae. Peptides 28, 109-118. crossref(new window)

Hong, S. H., Lee, K. S., Kwak, S. J., Kim, A. K., Bai, H., Jung, M. S., Kwon, O. Y., Song, W. J., Tatar, M. and Yu, K. 2012. Minibrain/Dyrk1a regulates food intake through the Sir2-FOXO-sNPF/NPY pathway in Drosophila and mammals. PLoS Genet. 8, e1002857. crossref(new window)

Kaneko, Y. and Hiruma, K. 2014. Short neuropeptide F (sNPF) is a stage-specific suppressor for juvenile hormone biosynthesis by corpora allata, and a critical factor for the initiation of insect metamorphosis. Dev. Biol. 393, 312-319. crossref(new window)

Kannan, K. and Fridell, Y. W. 2013. Functional implications of Drosophila insulin-like peptides in metabolism, aging, and dietary restriction. Front. Physiol. 288, 1-8.

Lee, K. S., You, K. H., Choo, J. K., Han, Y. M. and Yu, K. 2004. Drosophila short neuropeptide F regulates food intake and body size. J. Biol. Chem. 279, 50781-50789. crossref(new window)

Lee, K. S., Kwon, O. Y., Lee, J. H., Kwon, K., Min, K. J., Jung, S. A., Kim, A. K., You, K. H., Tatar, M. and Yu, K. 2008. Drosophila short neuropeptide F signalling regulates growth by ERK-mediated insulin signalling. Nat. Cell Biol. 10, 468-475. crossref(new window)

Lee, K. S., Hong, S. H., Kim, A. K., Ju, S. K., Kwon, O. Y. and Yu, K. 2009. Processed short neuropeptide F peptides regulate growth through the ERK-insulin pathway in Drosophila melanogaster. FEBS Lett. 583, 2573-2577. crossref(new window)

Mertens, I., Meeusen, T., Huybrechts, R., De Loof, A. and Schoofs, L. 2002. Characterization of the short neuropeptide F receptor from Drosophila melanogaster. Biochem. Biophys. Res. Commun. 297, 1140-1148. crossref(new window)

Nagata, S., Matsumoto, S., Nakane, T., Ohara, A., Morooka, N., Konuma, T., Nagai, C. and Nagasawa, H. 2012. Effects of starvation on brain short neuropeptide F-1, -2, and -3 levels and short neuropeptide F receptor expression levels of the silkworm, Bombyx mori. Front. Endocrinol. 3, 1-8.

Nässel, D. R., Kubrak, O. I., Liu, Y., Luo, J. and Lushchak, O. V. 2013. Factors that regulate insulin producing cells and their output in Drosophila. Front. Physiol. 252, 1-12.

Setzu, M., Biolchini, M., Lilliu, A., Manca, M., Muroni, P., Poddighe, S., Bass, C., Angioy, A. M. and Nichols, R. 2012. Neuropeptide F peptides act through unique signaling pathways to affect cardiac activity. Peptides 33, 230-239. crossref(new window)

Shang, Y., Donelson, N. C., Vecsey, C. G., Guo, F., Rosbash, M. and Griffith, L. C. 2013. Short neuropeptide F is a sleep-promoting inhibitory modulator. Neuron 80, 171-183. crossref(new window)