Advanced SearchSearch Tips
Alteration of plant hormones in transgenic rice (Oryza sativa L.) by overexpression of anti-apoptosis genes during salinity stress
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Plant Biotechnology
  • Volume 42, Issue 3,  2015, pp.168-179
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2015.42.3.168
 Title & Authors
Alteration of plant hormones in transgenic rice (Oryza sativa L.) by overexpression of anti-apoptosis genes during salinity stress
Ubaidillah, Mohammad; Safitri, Fika Ayu; Lee, Sangkyu; Park, Gyu-Hwan; Kim, Kyung-Min;
  PDF(new window)
We previously identified the rice gene, OsSAP, as an encoder of a highly conserved putative senescence-associated protein that was shown to have anti-apoptotic activity. To confirm the role of OsSAP in inducing abiotic stress tolerance in rice, we introduced OsSAP and AtBI-1, a plant homologue of Bax inhibitor-1, under the control of the CaMV 35S promoter into the rice genome through Agrobacterium-mediated transformation. The OsSAP transformants showed a similar chlorophyll index after salinity treatments with AtBI-1. Furthermore, we compared the effects of salinity stress on leaves and roots by examining the hormone levels of abscisic acid (ABA), jasmonic acid (JA), gibberellic acid (GA3), and zeatin in transformants compared to the control. With the exception of phytohormones, stress-induced changes in hormone levels putatively related to stress tolerance have not been investigated previously. Hormonal level analysis confirmed the lower rate of stress in the transformants compared to the control. The levels of ABA and JA in OsSAP and AtBI-1 transformants were similar, where stress rates increased after one week and decreased after a two week period of drought; there was a slightly higher accumulation compared to the control. However, a similar trend was not observed for the level of zeatin, as the decrease in the level of zeatin accumulation differed in both OsSAP and AtBI-1 transformants for all genotypes during the early period of salinity stress. The GA3 level was detected under normal conditions, but not under salinity stress.
Plant hormone;Rice response;Salinity stress;Anti-apoptosis genes;OsSAP;AtBI-1;
 Cited by
Roles of plant hormones and anti-apoptosis genes during drought stress in rice (Oryza sativa L.), 3 Biotech, 2016, 6, 2  crossref(new windwow)
Aloni R, Aloni E, Langhans M, Ullrich CI (2006) Role of cytokinin and auxin in shaping root architecture:regulating vascular differentiation, lateral root initiation, root apical dominance and root gravitropism. Ann Bot 97:883-893 crossref(new window)

Bari R, Jones JDG (2009) Role of hormones in plant defense responses. Plant Mol Biol 69:473-488 crossref(new window)

Bolduc N, Ouellet M, Pitre F, Brisson LF (2003) Molecular characterization of two plant BI-1 homologues which suppress Bax-induced apoptosis in human 293 cells. Planta 216:377-386

Browse J. 2009. Jasmonate passes muster:a receptor and targets for the defense hormone. Annu. Rev. Plant Biol 60:183-205 crossref(new window)

Cheng WH, Endo A, Zhou L, Penney J, Chen HC, Arroyo A, Leon P, Nambara E, Asami T, Seo M, Koshiba T, Sheen J (2002) A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell 14:2723-2743 crossref(new window)

Christmann A, Hoffmann T, Teplova I, Grill E, Muller A (2005) Generation of active pools of abscisic acid revealed by in vivo imaging of water-stressed Arabidopsis. Plant Physiol 137:209-219 crossref(new window)

Eichmann R, Schultheiss H, Kogel KH, Huckelhoven R (2004) The barley apoptosis Suppressor homologue Bax inhibitor-1 compromises non host penetration resistance of barley to the in appropriate pathogen Blumeriagraminisf Sp. Tritici. Mol. Plant-Microbe Interact 17:484-490 crossref(new window)

Flowers TJ (2004) Improving crop salt tolerance. J Exp Bot 55:307-319 crossref(new window)

Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, Yamaguchi-Shinozaki K, Shinozaki K (2006) Crosstalk between abiotic and biotic stress responses:A current view from the points of convergence in the stress signaling networks. Curr Opin Plant Biol 9:436-442 crossref(new window)

Fukuda A, Tanaka Y (2006) Effects of ABA, auxin, and gibberellin on the expression of genes for vacuolar $H^+$- inorganic pyrophosphatase, $H^+$-ATPase subunit A, and $Na^+/H^+$ antiporter in barley. Plant Physiol Bioch 44:351-358 crossref(new window)

Golldack D, Li C, Mohan H, Probst N (2013) Gibberellins and abscisic acid signal crosstalk:living and developing under unfavorable conditions. Plant Cell Repots 32:1007-1016 crossref(new window)

Hanada M, Aime-Sempe C, Sato T, Reed JC (1995) Structure function analysis of Bcl-2 protein. Identification of conserved domains important for homodimerization with Bcl-2 and heterodimerization with Bax. J. Biol. Chem. 270:11962-11969 crossref(new window)

Harb A, Krishnan A, Ambavaram MM, Pereira A (2010) Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth. Plant Physiol 154:1254-1271 crossref(new window)

Harrison MA (2012) Cross-talk between pytohormone signaling pathways under both optimal and stressful environmental condition. In:Phytohormone and abiotic stress tolerance in plants. Springers., Berlin/Heidelberg/New York., pp 49-76

Hoeberichts FA, Woltering EJ (2003) Multiple mediators of plant programmed cell death:interplay of conserved cell death mechanisms and plant-specific regulators. Bioessays 25:47-57 crossref(new window)

Huckelhoven R (2004) Bax inhibitor-1, an ancient cell death suppressor in animals and plants with prokaryotic relatives. Apoptosis 9:299-307 crossref(new window)

Isbat M, Zeba N, Kim SR, Hong CB (2009) A Bax inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transformants tobacco. J. Plant Physiol. 166:1685-1693 crossref(new window)

Kawai-Yamada M, Jin L, Yoshinaga K, Hirata A, Uchimiya H (2001) Mammalian Bax-induced plant cell death can be down Regulated by over-expression of Arabidopsis Bax Inhibitor-1 (AtBI-1). Proc. Natl. Acad. Sci. U.S.A. 98:12295-12300 crossref(new window)

Kawai-Yamada M, Yoshinaga K, Ogawa K, Ihara-Ohori Y, Uchimiya H (2005a) Oxidative stress and plant cell death suppressors. Plant Biotechnol 22:419-422 crossref(new window)

Kawai-Yamada M, Ohori Y, Uchimiya H (2004) Dissection of Arabidopsis BAX inhibitor-1 suppressing BAX-., hydrogen peroxide-., and salicylic acid-induced cell death. Plant Cell 16:21-32 crossref(new window)

Kawai-Yamada M, Saito Y, Jin L, Ogawa T, Kim KM, Yu LH, Tone Y, Hirata A, Umeda M, Uchimiya H (2005b) A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae. J Biol Chem 280:39468-39473 crossref(new window)

Kissoudis C, van de Wiel C, Visser RG, van der Linden G (2014) Enhancing crop resilience to combined abiotic and biotic stress through the dissection of physiological and molecular crosstalk. Front Plant Sci 5:207

Kroemer G (1997) Theproto-oncogene Bcl-2 and it srolein regulating apoptosis. Nat Med 3:614-620 crossref(new window)

Lacomme C, Santa Cruz S (1999) Bax-induced cell death in tobacco is similar to hypersensitive response. Proc Natl Acad Sci USA 96:7956- 7961 crossref(new window)

Lehmann J, Atzorn R, Bruckner C, Reinbothe S, Leopold J, Wasternack C, Parthier B (1995) Accumulation of Jasmonate, abscisic acid, specific transcripts and proteins in osmotically stressed barley leaf segments. Planta 197:156-162

Munns R (2002) Comparative physiology of salt and water stress. Plant, Cell and Environment 25:239-250 crossref(new window)

Munns R (2005) Genes and salt tolerance:bringing them together. New phytol 167:645-663 crossref(new window)

Pan X, Ruth W, Xuemin W (2010) Quantitative analysis of major plant hormones incrude plant extracts by high-performance liquid chromatography-mass spectrometry. Nat Protoc 56:986-992

Pedranzani H, Racagni G, Alemano S, Miersch O, Ramirez I, Pena Cortes H., Machado-Domenech E, Abdala G (2003) Salt tolerant tomato plants show increased levels of jasmonic acid. Plant Growth Regul 41:149-158 crossref(new window)

Pieterse CMJ, Leon-Reyes A, Van der Ent S, Van Wees SCM (2009) Networking by small-molecule hormones in plant immunity. Nat Chem Biol 5:308-316 crossref(new window)

Sanchez P, de Torres Zabala M, Grant M (2000) AtBI-1, a plant homologue of Bax inhibitor-1, suppresses BAX-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge. Plant J 21:393-399 crossref(new window)

Sato T, Hanada M, Bodrug S, Irie S, Iwama N, Boise LH, Thompson CB, Golemis E, Fong L, Wang HG, Reed J (1994) Interactions among members of the bcl-2 protein family analyzed with a yeast-hybrid system. Proc Natl Acad Sci USA 91:9238-9242 crossref(new window)

Scarfi S, Fresia C, Ferraris C, Bruzzone S, Fruscione F, Usai C, Benvenuto F, Magnone M, Podesta M, Sturla L, Guida L, Albanesi E, Damonte G, Salis A, De Flora A, Zocchi E (2009) The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose. Stem Cells 10:2469-77

Schwartz SH, Qin X, Zeevaart JA (2003) Elucidation of the indirect pathway of abscisic acid biosynthesis by mutants, genes, and enzymes. Plant Physiol 131:1591-1601 crossref(new window)

Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J (2007) JAZ repressor proteins are targets of the SCF (COI1) complex during jasmonate signalling. Nature 448:661-665 crossref(new window)

Ubaidillah M, Kim KA, Kim YH, Lee IJ, Yun BW, Kim DH, Loake GJ, Kim KM (2013) Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast. Mol Biol Rep 40:6113-6121 crossref(new window)

Watanabe N, Lam E (2008) BAX inhibitor-1 modulates endoplasmic reticulum stress-mediated programmed cell death in Arabidopsis. J Biol Chem 283:3200-3210 crossref(new window)

Yeo AR (2007). Salinity. In:Yeo AR, Flowers TJ, eds. Plant solute transport. Oxford:Blackwell Publishing Ltd, 340-365

Zhang J, Jia W, Yang J, Ismail AM (2006) Role of ABA in integrating plant responses to drought and salt stresses. Field Crop. Res. 97:111-119 crossref(new window)