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Establishment of detection methods for approved LMO in Korea
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  • Journal title : Journal of Plant Biotechnology
  • Volume 42, Issue 3,  2015, pp.196-203
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2015.42.3.196
 Title & Authors
Establishment of detection methods for approved LMO in Korea
Seol, Min-A; Lee, Jung Ro; Choi, Wonkyun; Jo, Beom-Ho; Moon, Jeong Chan; Shin, Su Young; Eum, Soon-Jae; Kim, Il Ryong; Song, Hae-Ryong;
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 Abstract
AbstractLiving modified organisms (LMO) are one of the most widespread products of modern biotechnology after DNA discovery. Due to the decline of grain self-sufficiency rate and the increase of reliance on LMO imports in Korea, a series of concerns with regard to safety of living modified(LM) crops has been raised. The aim of this study is to establish the detection methods for unintentional release or growing of LMO plants in environmental conditions. To detect LM crop events, general concepts of specific primer design and PCR conditions were provided by the Joint Research Centre (JRC). The certified reference materials of seven LM events (4 soybean, 2 cotton and 1 corn) were obtained from the Institute for Reference Materials and Measurements (IRMM) and the American Oil Chemists' Society (AOCS). Genomic DNA from seven LM events were purified and PCR amplifications were carried out by using individual event-specific primer sets. LM-specific PCR products of all seven events were efficiently amplified by our methods. The results indicate that the established detection method for LMOs is suitable as a scientific tool to monitor whether the crops found in natural environments are LMOs.
 Keywords
living modified organism (LMO);detection method;event specific primer;polymerase chain reaction (PCR);
 Language
Korean
 Cited by
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