Advanced SearchSearch Tips
Effects of Brassica rapa SHI-RELATED SEQUENCE overexpression on petunia growth and development
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Plant Biotechnology
  • Volume 42, Issue 3,  2015, pp.204-214
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2015.42.3.204
 Title & Authors
Effects of Brassica rapa SHI-RELATED SEQUENCE overexpression on petunia growth and development
Hong, Joon Ki; Suh, Eun Jung; Lee, Su Young; Song, Cheon Young; Lee, Seung Bum; Kim, Jin A; Lee, Soo In; Lee, Yeon-Hee;
  PDF(new window)
SHI-RELATED SEQUENCE (SRS) genes are plant-specific transcription factors that contain a zinc-binding RING finger motif, which play a critical role in plant growth and development. Among Brassica rapa SRS genes, BrSRS7 and BrLRP1 genes, isolated from shoot apical regions are important regulators of plant growth and development. In order to explore the function of BrSRS genes in horticultural plant growth and development, two constructs containing BrSRS7 and BrLRP1 under the control of a cauliflower mosaic virus 35S promoter were introduced into petunia by Agrobacterium-mediated transformation. The resulting transgenic plants were dwarf and compact plants with reduced plant height and diameter. Additionally, these transgenic plants had upward-curled leaves of narrow width and short internodes. Interestingly, the flower shapes of petunia were different among transgenic plants harboring different kinds of SRS genes. These phenotypes were stably inherited through generations and . Semi-quantitative RT-PCR analyses of transgenic plants revealed that BrSRS7 and BrLRP1 regulate expression of gibberellin (GA)- and auxinrelated genes, PtAGL15- and PtIAMT1-related, involved in shoot morphogenesis. These results indicate that the overexpression of BrSRS7 and BrLRP1 genes suppressed the growth and development of petunia by regulating expression of GA- and auxin-related genes. From these data, we deduce that BrSRS7 and BrLRP1 genes play an important role in the regulation of plant growth and development in petunia. These findings suggest that transformation with the BrSRS genes can be applied to other species as a tool for growth retardation and modification of plant forms.
Short internodes;Transcription factor;Transgenic plant;Retardation;Morphogenesis;
 Cited by
Coles JP, Phillips AL, Croker SJ, Garcia-Lepe R, Lewis MJ, Hedden P (1999) Modification of gibberellin production and plant development in Arabidopsis by sense and antisense expression of gibberellin 20-oxidase genes. The Plant J 17:547-556 crossref(new window)

Chandler SF, Sanchez C (2012) Genetic modification; the development of transgenic ornamental plant varieties. Plant Biotech J 10:891-903 crossref(new window)

De Castro VL, Goes KP, Chiorato SH (2004) Developmental toxicity potential of paclobutrazol in the rat. Int J Environ Health Res 14:371-380 crossref(new window)

Eckardt NA (2002) Foolish seedlings and DELLA regulators: the functions of rice SLR1 and Arabidopsis RGL1 in GA signal transduction. Plant Cell 14:1-5 crossref(new window)

Eklund DM, Staldal V, Valsecchi I, Cierlik I, Eriksson C, Hiratsu K,Ohme-Takagi M, Sundstrom JF, Thelander M, Ezcurra I et al. (2010) The Arabidopsis thaliana STYLISH1 protein acts as a transcriptional activator regulating auxin biosynthesis. Plant Cell 22:349-363 crossref(new window)

Fleet CM, Sun TP (2005) A DELLAcate balance: the role of gibberellins in plant morphogenesis. Curr Opin Plant Biol 8:77-85 crossref(new window)

Fridborg I, Kuusk S, Moritz T, Sundberg E (1999) The Arabidopsis dwarf mutant shi exhibits reduced gibberellin responses conferred by overexpression of a new putative zinc finger protein. Plant Cell 11: 1019-1032 crossref(new window)

Fridborg I, Kuusk S, Robertson M, Sundberg E (2001) The Arabidopsis protein SHI represses gibberellin responses in Arabidopsis and barley. Plant Physiol 127:937-948 crossref(new window)

Fu X, Sudhakar D, Peng J, Richards DE, Christou P, Harberd NP (2001) Expression of Arabidopsis GAI in transgenic rice represses multiple gibberellin responses. Plant Cell 13:1791-1802 crossref(new window)

Hong JK, Kim JS, Kim JA, Lee SI, Lim MH, Park BS, Lee YH (2010) Identification and characterization of SHI family genes from Brassica rapa L. ssp. Pekinensis. Genes & Genomics 32: 309-317 crossref(new window)

Hong JK, Kim JA, Kim, JS, Lee SI, Koo BS, Lee Y-H (2012) Overexpression of Brassica rapa SHI-RELATEDSEQUENCE genes suppresses growth and development in Arabidopsis thaliana, Biotechnology letters 34:561-156

Islam MA, Lutken H, Haugslien S, Blystad D-R, Torre S, Rolcik J (2013) Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants, PLoS One 8: e53377 crossref(new window)

Kazaz S, Atilla AM, Kilic S, Ersoy N (2010) Effects of day length and daminozide on the flowering, some quality parameters and chlorophyll content of Chrysanthemum morifolium Ramat. Sci Res Essays 5(21):3281-3288

Kim SG, Lee S, Kim YS, Yun DJ, Woo JC, Park CM (2010) Activation tagging of an Arabidopsis SHI-RELATED SEQUENCE gene produces abnormal anther dehiscence and floral development. Plant Mol Biol 74:337-351 crossref(new window)

Koornneef M, Elgersma A, Hanhart CJ, van Loenen-Martinet EP, van Rijn L, Zeevaart JAD (1985) A gibberellin insensitive mutant of Arabidopsis thaliana. Physiol Plant 65:33-39 crossref(new window)

Krysan PJ, Young JC, Sussman MichaelR (1999) T-DNA as an Insertional Mutagen in Arabidopsis. The Plant Cell 11: 2283-2290 crossref(new window)

Kuusk S, Sohlberg JJ, Magnus Eklund D, Sundberg E (2006) Functionally redundant SHI family genes regulate Arabidopsis gynoecium development in a dose-dependent manner. The Plant J 47:99-111 crossref(new window)

Li LC, Kang DM, Chen ZL, Qu LJ (2007) Hormonal regulation of leaf morphogenesis in Arabidopsis. J Integr Plant Biol 49: 75-80 crossref(new window)

Liscum L, Reed JW (2002) Genetics of Aux/IAA and ARF action in plant growth and development. Plant Mol Biol 49:387-400 crossref(new window)

Lutken H, Clarke JL, Muller R (2012) Genetic engineering and sustainable production of ornamentals:current status and future directions, Plant Cell Rep 31:1141-1157 crossref(new window)

Lutken H, Jensen SJ, Topp SH, Mibus H, Muller R, Rasmussen SK (2010) Production of compact plants by overexpression of AtSHI in the ornamental Kalanchoe, Plant Biotechnology Journal 8:211-222 crossref(new window)

Olszewski N, Sun TP, Gubler F (2002) Gibberellin signaling: Biosynthesis, catabolism, and response pathways. Plant Cell 14 (suppl.):S61-S80 crossref(new window)

Petty LM, Thomas B, Jackson SD, Harberd N (2001) Manipulating the gibberellin response to reduce plant height in Chrysanthemum morifolium. Acta Hortic 560:87-90

Ryu H-S, Ryoo N, Jung K-H, An G, Jeon J-S (2010) Rice functional genomics using T-DNA mutants. J Plant Biotechnol 37:133-143 crossref(new window)

Topp SH, Rasmussen SK, Mibus H, Sander L (2009) A search for growth related genes in Kalanchoe blossfeldiana. Plant Physiol Biochem 47:1024-1030 crossref(new window)

Sohlberg JJ, Myrenas M, Kuusk S, Lagercrantz U, Kowalczyk M, Sandberg G, Sundberg E (2006) STY1 regulates auxin homeostasis and affects apical-basal patterning of the Arabidopsis gynoecium. The Plant J 47:112-123 crossref(new window)

Song CY (2009a) Selection of pure lines with various growth and flowering characteristics of spreading petunia, Petunia $\times$ hybrida. Flower Res J 17(2):128-136

Song CY (2009b) Correlation and combining ability of plant spreading characteristics in F1 hybrid by diallel cross in Petunia hybrida. Flower Res J 17(3):179-184

Sorensen MT, Danielsen V (2006) Effects of the plant growth regulator, chlormequat, on mammalian fertility. Int J Androl 29:129-133 crossref(new window)

Staldal V, Sohlberg JJ, Eklund DM. Ljung K, Sundberg E (2008) Auxin can act independently of CRC, LUG, SEU, SPT and STY1 in style development but not apical-basal patterning of the Arabidopsis gynoecium. New Phytol 180:798-808 crossref(new window)

Wen C-K and Chang C (2002) Arabidopsis RGL1 encodes a negative regulator of gibberellin responses. Plant Cell 14: 87-100 crossref(new window)

Zawaski C, Kadmiel M, Ma C, Gai Y, Jiang X, Strauss SH, Busov VB (2011) SHORT INTERNODES-like genes regulate shoot growth and xylem proliferation in Populus, New Phytologist 191:678-691 crossref(new window)

Zhu LH, Li XY, Welander M (2008) Overexpression of the Arabidopsis gai gene in apple significantly reduces plant size. Plant Cell Rep 27:289-296 crossref(new window)