Advanced SearchSearch Tips
Effect of explant's position and culture method on shoot proliferation and micro-cuttings for a rare and endangered species, Abeliophyllum distichum Nakai
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Plant Biotechnology
  • Volume 42, Issue 3,  2015, pp.228-234
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2015.42.3.228
 Title & Authors
Effect of explant's position and culture method on shoot proliferation and micro-cuttings for a rare and endangered species, Abeliophyllum distichum Nakai
Lee, Na Nyum; Kim, Ji-Ah; Kim, Yong-Wook; Choi, Yong Eui; Moon, Heung Kyu;
  PDF(new window)
Using either the apical or axillary bud of the endangered species Abeliophyllum distichum Nakai, we tested the effect of bud position and culture method on shoot proliferation and rooting. In shoot proliferation, the axillary bud explant was more effective than the apical bud and the effect was fostered by BA treatment, whereas no differences were observed in shoot elongation by the explant position. Spontaneous rooting was observed in the MS basal medium and resulted in conspicuous differences in the explant position : more than 80% in apical bud explant and 28% in axillary bud explant was achieved, respectively. The positional effects were also observed in BA pre-treatments: generally vertical culture method appeared to be better in shoot proliferation, growth, and rooting than that of the horizontal culture method regardless of the BA pre-treatment duration. The highest shoot multiplication was achieved through the vertical culture method with axillary bud explant, whereas the best shoot elongation and rooting was obtained using the vertical culture method with the apical bud explant. Apical bud explant was superior to axillary bud explant in ex vitro micro-cuttings and revealed a significant difference in shoot growth and root development. The above results suggest that explant position and culture method influence the efficiency of micropropagation for a rare and endangered plant Abeliophyllum distichum.
Abeliophyllum distichum Nakai;Apical or axillary bud explants;Cultural method;Micropropagation;
 Cited by
Effect of kinds of medium and plant growth regulators for plantlets regeneration by bud culture in Disporum sessile, Journal of Plant Biotechnology, 2017, 44, 1, 42  crossref(new windwow)
Azamal H, Mohinder P (2007) Effect of branch position and auxin treatment on clonal propagation of Tectona grandis Linn. f. New Forest 34:223-233 crossref(new window)

Bhardwaj DR, Mishra VK (2005) Vegetative propagation of Ulmus villosa: Effects of plant growth regulators, collection time, type of donor and position of shoot on adventitious root formation in stem cuttings. New Forest 29:105-116 crossref(new window)

Chen UC, Hsia CN, Yeh MS, Agrawal DC, Rsay HS (2006) In vitro micropropagation and ex vitro acclimation of Bulpleurum kaoi- an endangered medicinal plant native to Taiwan. In Vitro Cell Dev Biol Plant 42:128-133 crossref(new window)

Debergh PC (1988) Micropropagation of woody species-state of the art on in vitro aspects. Acta Hortic 227:287-295

Duong Tan Nhut, Bui Van Le, Seiichi Fukai, Michio Tanaka, K. Tran Thanh Van (2001) Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regul 33:59-65 crossref(new window)

Duong Tan Nhut, Truong Thi Thuy An, Nguyen Thi Dieu Huong, Nguyen Trinh Don, Nguyen Thanh Hai, Nguyen Quoc Thien, Nguyen Hong Vu (2007) Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture. Sci Hortic-Amaterdam 111:146-151 crossref(new window)

El-Morsy AA, Millet B (1996) Rhythmic growth and optimization of micropropagation: The effect of excision time and position of axillary buds on in vitro culture of Citrus aurantium L. Ann Bot-London 78:197-202 crossref(new window)

Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica. an endangered, medicinally important plant. Plant Biotech Rep 1:155-161 crossref(new window)

Gomes GAC, Paiva R, Paiva PDO, Santiago EJAD (2003) Plant regeneration from callus cultures of Maclura tinctoria, an endangered woody species. In Vitro Cell Dev Biol Plant 39:293-295 crossref(new window)

Han MS, Park SY, Moon HK, Kang YJ (2010) Micropropagation of a rare tree species, Empetrum nigrum var. japonicum K. Koch via axillary bud culture. J Kor Forest Soci 99(4): 568-572

Hsueh-Shih Lin, Marjo J, De Jeu, Evert Jacobsen (1998) Formation of shoots from leaf axils of Alstroemeria: The effect of the position on the stem. Plant Cell Tiss Org 52:165-169 crossref(new window)

Husen A (2004) Clonal propagation of Dalbergia sissoo Roxb. By softwood nodal cuttings: Effects of genotypes, application of IBA and position of cuttings on shoots. Silvae Genet 53:50-55 crossref(new window)

Kim CS, Kim ZS (2012) Effects of cutting time, auxin treatment and cutting position on rooting of the green-wood cuttings and growth characteristics of transplanted cuttings in the adult Prunus yedoensis. Kor J Horticul Sci Tech 30(2):129-136 crossref(new window)

Lardet, Enjalric F, Carron MP (1990) Hevea brasiliensis (Mull. Arg.) apex cultures. Influence of the morphogenetical stage and explant age. Comptes Rendus de l'AcadeUmie des Sciences Serie III-Sciences de la Vie 310(5):195-202

Leakey RRB (1983) Stock Plant factors affecting root initiation in cuttings of Triplochiton Scleroxylon K. Schum an indigenous hardwood of West Africa. J Hortic Sci Biotech 58:227-290

Lee NN, Choi YE, Moon HK (2014) Effect of LEDs on shoot multiplication and rooting of rare plant Abeliophyllum distichum Nakai. J Plant Biotechnol 41:94-99 crossref(new window)

Lee TB (1976) Illustrated flora of Korea. Hyangmun Press

Lee TB (1990) Conservation of threatened plants in Korea. Bull Kwanak Arboretum. 3:190-196

Maria Papafotiou, Aekaterini N, Martini (2009) Effect of position and orientation of leaflet explants with respect to plant growth regulators on micropropagation of Zamioculcas zamiifolia Engl. (ZZ). Sci Hortic-Amsterdam 120:115-120 crossref(new window)

Matin MA (1989) Carbon economy during rooting of cuttings of Nauclea diderrichii (De. Wild and Th. Dus.) Merill. M. Phil thesis, University of Edinburgh, United Kingdom

Minal Mhatre, Bapat VA, Rao PS (1985) Regeneration of plants from the culture of leaves and axillary buds in mulberry (Morus indica L.). Plant Cell Rep 4:78-80 crossref(new window)

Mitsukuri K, Mori G, Johkan M, ShimadaY, Mishiba K-I, Morikawa T, Masayuki Oda (2009) Effects of explant position and dark treatment on bud formation in floret culture of Ponerorchis graminifolia Rchb.f. Sci Hortic-Amsterdam 121:243-247 crossref(new window)

Moon HK, Kim YW (2008) In vitro propagation of a rare and endangered species, Echinosophora koreensis Nakai, by axillary bud culture. J Plant Biotechnol 35(3):229-234 crossref(new window)

Moon HK, Noh EW, Ha YM, Shim KK (2002) Micropropagation of Juvenile and mature tree of Corylolopsis coreana by axillary bud culture. J Plant Biotechnol 29(2):117-121 crossref(new window)

Moon HK, Suk GY, Kim SC (1997) Micropropagation of a rare species, Forsythia saxatilis N. through tissue culture. J Kor Forest Soci 86(4):430-434

Moon HK, Suk GY, Kwon YJ, Son SH (1999) Micropropagation of a rare species, Abeliophyllum distichum Nakai, via axillary bud culture. Kor J plant tiss culture 26(2):133-136

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. J Plant Physiol 15:473-497 crossref(new window)

Negash L (2002) Successful vegetative propagation techniques for the threatened African pencil cedar (Juniperus procera Hoechst. Ex Endl.). Forest Ecol Manag 161:53-64 crossref(new window)

Ofori D, Newton AC, Leakey RRB, Grace J (1997) Vegetative propagation of Milicia excelsa by leafy stem cuttings: Effects of maturation, coppicing, cutting length and position on rooting ability. J Tropical Forest Sci 10(1):115-129

Paek KY (1998) Commercial aspects and problems in micropropagation. The 12th Symposium on Plant Biotechnology. Korea-Japan Joint Symposium. Frontier Researches in Plant Biotechnol 3:152-156

Park SY, Ahn JK, Lee WY (2003) High frequency shoot induction from root segments of Albizzia coreana. J Kor Forest Soci 92:626-631

Pereira AMS, Moro JR, Cerdeira RMM, Franca SC (1995) Effect of phytoregulators and physiological characteristics of the explants on micropropagation of Maytenus ilicifolia. Plant Cell Tiss Org 42:295-297 crossref(new window)

Poupard C, Chauviere M, Monteuuis O (1994) Rooting Acacia mangium cuttings: Effects of age, within-shoot position and auxin treatment. Silvae Genet 43(4):226-231

Sanjaya, Muthan B, Rathore TS, Rai VR (2006) Micropropagation of an endangered Indian sandalwood (Santalum album L.). J Forest Res 11:203-209 crossref(new window)

Youn Y, Lee SK, Park JI (1992) In vitro propagation of a rare species-Berchemia berchemiaefolia. Res Rep For Gen Res Inst Korea 28:63-67

Vendrame WA, Maguire I, Carvalho VS (2007) In vitro propagation and plantlet regeneration from Doritaenopsis purple gem 'Ching Hua' flower explants. HortScience 42: 1256-1258

Viemont J-D (1984) Les bruyeres in vitro. IV. Influence de la croissance rythmique sur l'evolution des bourgeons axillaires de l'Erica x darleyensis. Annales des Sciences Naturelles. Botanique. Paris 6:237-244