Publisher : The Korean Society of Plant Biotechnology
DOI : 10.5010/JPB.2015.42.4.370
Title & Authors
Factors influencing shoot regeneration from petal explant in spray mum 'Purple ND' Lee, Hyun Suk; Park, Hyun Rho; Kim, Hyun seak; Kim, Chang Kil;
This experiment compared the regeneration conditions of the radiation mutant spray chrysanthemum 'purple ND'. The four different flower blooming stages (S1: 10% opened flower, S2: 30% opened flower, S3: 50% opened flower, and S4: 70% opened flower) and different petal parts (TBOP: the basal of petal and TEOP: the end of petal) were used to compare regeneration conditions between plants grown in MS medium supplemented with IAA and BAP. The highest adventitious shooting rate was identified in plants grown on the IAA and BAP when using the end of petal at the S2 stage. It displayed 79.2% regeneration and produced 33.4 shoots. Rooted plantlets were successfully established in the greenhouse, showing the same morphological characteristics of vegetative and reproductive organs with those of the mother plant. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions.
Annadana S, Rademaker W, Ramanna M, Udayakumar M, de Jong J (2000) Response of stem explants to screening and explant source as a basis for methodical advancing of regeneration protocols for chrysanthemum. Plant Cell Tissue Organ Cult. 62:47-55.
Broerjes C (1966) Mutation breeding of chrysanthemum. Euphytica 15:156-162.
Choi KJ (2002) International union for the protection of new varieties of plant (UPOV) and its 1991 UPOV convention. Kor. J. Hort. Sci. Technol. 20:151-159.
Datta SK, Chakrabarty D, Mandal AKA (2001) Gamma rayinduced genetic manipulations in flower colour and shape in Dendranthema grandiflorum and their management through tissue culture. Plant Breed. 120:91-92.
Gilissen LJW, van Staveren MJ, Hakkert JC, Smulders MJM (1996) Competence for regeneration during tobacco internodal development: Involvement of plant age, cell elongation stage, and degree of polysomaty. Plant Physiol. 111:1243-1250.
Himstedt JP, Jacobsen HJ, Fisher-Kluver G (2001) Shoot regeneration from stem and leaf explant of chrysanthemum (Dendrathema $\times$ grandiflorim). Acta Hortic. 560-421-424.
Mandal AKA, Datta SK (2005) Direct somatic embryogenesis and plant regeneration from ray florets of chrysanthemum. Biol. Plant. 49:29-33.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15:473-479.
Park IS, Lee GJ, Kim DS, Chung SJ, Kim JB, Song HS, Goo DH, Kang SY (2007) Mutation breeding of a spray chrysanthemum 'Argus' by gamma-ray irradiation and tissue culture. Flower Res. J. 15:52-57.
Lu C, Vasil IK, Ozias-Akins P (1982) Somatic embryogenesis in Zea mays L. Theor. Appl. Genet. 62:109-112.
Petty LM, Harbred NP, Carre IA, Thomas B, Jackson SD (2003) Expression of the Arabidopsis gai gene under its own promoter causes a reduction in plant height in chrysanthemum by attenuation of the gibberellin response. Plant Sci. 164: 175-182.
Rural Development Administration (RDA) (2002) Handbook of farming standard-131. chrysanthemum cultivation. Government publications registration number (Korea) 11-139000-001153-14. RDA, Suwon, Korea.
Waseem K, Jilani MS, Khan MS (2009) Rapid plant regeneration of chrysanthemum (chrysanthemum morifolium L.) through shoot tip culture. Afr. J. Biotechnol. 8:1871-1877.
Zalewska M, Lema-Ruminska J, Miler N (2007) In vitro propagation using adventitious buds techique as a source of new variability in chrysanthemum. Sci. Hortic. 113:70-73.