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Plant regeneration from hypocotyls explants of Astragalus sinicus L.
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  • Journal title : Journal of Plant Biotechnology
  • Volume 42, Issue 4,  2015, pp.396-400
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2015.42.4.396
 Title & Authors
Plant regeneration from hypocotyls explants of Astragalus sinicus L.
Park, Min Sun; Choi, Pil Son;
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To investigate the optimal conditions for shoot organogenesis in Astragalus sinicus L., hypocotyl explants were cultured in Murashige & Skoog's (MS) medium supplemented with 0.1, 1.0, 2.0, or 4.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) for 6 weeks. 2,4-D concentration significantly effected morphogenesis: some produced calli with adventitious shoots and roots, some produced calli with adventitious roots, some produced only calli, and some produced deep-brownish calli with roots. The formation of calli with shoots and/or roots was observed at lower levels of 2,4-D, whereas calli without shoots or with deep-brownish roots were formed after treatment with higher levels of 2,4-D. Also, a shoot organogenesis ability of callus clones was observed after treatment with medium with 0.1 or 1.0 mg/L 2,4-D grown in MS medium with combinations of benzyl adenine (BA) and 2,4-D for 4 weeks. Medium with a combination of BA and 2,4-D was effective for shoot formation, whereas root organogenesis from calli decreased. The greatest amount of shoot formation was obtained when calli were cultured in MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BA. Upon shoot transfer into 1/2 MS basal medium, plantlets developed, and the plantlets grew well in soil in a greenhouse.
Astragalus sinicus;Hypocotyl explants;Plantlets;Shoot organogenesis;
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