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Genetic diversity analysis of Glycyrrhiza uralensis using 8 novel polymorphic microsatellite markers
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  • Journal title : Journal of Plant Biotechnology
  • Volume 43, Issue 2,  2016, pp.174-180
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2016.43.2.174
 Title & Authors
Genetic diversity analysis of Glycyrrhiza uralensis using 8 novel polymorphic microsatellite markers
Um, Yurry; Jin, Mei-Lan; Lee, Yi; Hur, Mok; Cha, Seon Woo; Jung, Chan Sik; Kim, Seong Min; Lee, Jeong-Hoon;
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 Abstract
Licorice plant (Glycyrrhiza spp.) is an important herb, but the major portion of the national demand is imported to Korea because the domestic production base is vulnerable. We performed basic molecular breeding research for domestic cultivation and production. All publicly available G. uralensis EST sequences, which totaled 56,089, were assembled into 4,821 unigenes and examined for microsatellites. Eight polymorphic microsatellite loci were identified and 16 G. uralensis and 6 G. glabra accessions, which were collected from different locations, were genotyped using the microsatellites. Genetic diversity within the accessions was estimated by construction of a dendrogram. The dendrogram was clustered into two groups. The results showed that there is a correlative genetic relationship between species. The microsatellite markers were found to be useful for diversity analysis as they are able to successfully distinguish the Glycyrrhiza accessions.
 Keywords
Glycyrrhiza;Microsatellites;Genetic diversity;
 Language
English
 Cited by
 References
1.
Asl MM, Husseyinzadeh H (2008) Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds. Phytother Res 22:709-724 crossref(new window)

2.
Chevreux B, Pfisterer T, Drescher B (2004) Using the miraEST Assembler for Reliable and Automated mRNA Transcript Assembly and SNP Detection in Sequenced ESTs. Genome Res 14:1147-1159 crossref(new window)

3.
Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, Robles M (2005) Blast2GO: a Universal Tool for Annotation, Visualization and Analysis in Functional Genomics Research. Bioinformatics 21:3674-3676 crossref(new window)

4.
Erayman M, Ilhan E, GUZEL Y, Eren AH (2014) Transferability of SSR Markers from Distantly Related Legumes to Glycyrrhiza species. Turk J Agric For 38:32-38 crossref(new window)

5.
Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK (2011) Microsatellite markers: An Overview If the Recent Progress in Plants. Euphytica 177:309-334 crossref(new window)

6.
Khan S, Mirja KJ, Tayyab M, Abdin MZ (2009) RAPD Profile for Authentication of Medicinal Plant Glycirrhiza glabra L. Medicinal Aromatic Plant Sci Biotech 3:8-51

7.
Koressaar T, Remm M (2007) Enhancements and Modifi Cations of Primer Design Program Primer3. Bioinformatics (Oxford, England) 23:1289-1291 crossref(new window)

8.
Liu KJ, Muse SV (2005) PowerMarker: An Integrated Analysis Environment for Genetic Marker Analysis. Bioinformatics 21:2128-2129 crossref(new window)

9.
Liu Y, Zhang P, Song M, Hou J, Qing M, Wang W, Liu C (2015) Transcriptome Analysis and Development of SSR Molecular Markers in Glycyrrhiza uralensis Fisch. PloS one 10:e0143017 crossref(new window)

10.
Morin RD, Bainbridge M, Fejes A, Hirst M, Krzywinski M, Pugh TJ, McConald H, Varhol R, Jones SJM, Marra MA (2008) Profiling the HeLa S3 Transcriptome using Randomly Primed cDNA and Massively Parallel Short-read Sequencing. Biotechniques 45:81 crossref(new window)

11.
Rohlf FJ (2000) Phylogenetic Models and Reticulations. J Classif 17:185-189 crossref(new window)

12.
Rohlf FJ (2004) NTSYS-pc Ver. 2.11 T. Exter Software, New York

13.
Roldan-Ruiz I, Dendauw J, Van Bockstaele E, Depicker A, De Loose M (2000) AFLP Markers Reveal High Polymorphic Rates in Ryegrasses (Lolium spp.). Mol Breeding 6:125-134 crossref(new window)

14.
Sorrells ME, Wilson WA (1997) Direct Classification and Selection of Superior Alleles for Crop Improvement. Crop Sci 37:691-697 crossref(new window)

15.
Sudo H, Seki H, Sakurai N, Suzuki H, Shibata D, Toyoda A, Totoki Y, Sakaki Y, Iida O, Shibata T, Kojoma M, Muranaka T, Saito K (2009) Expressed Sequence Tags from Rhizomes of Glycyrrhiza uralensis. Plant Biotechnol 26:105-107 crossref(new window)

16.
Temnykh S, DeClerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch SR (2001) Computational and Experimental Analysis of Microsatellites in Rice (Oryza sativa L.): Frequency, Length Variation, Transposon Associations, and Genetic Marker Potential. Genome Res 11:1441-1452 crossref(new window)

17.
Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG (2012) Primer3-New Capabilities and Interfaces. Nucleic Acids Res 40:e115-e115 crossref(new window)

18.
Varshney RK, Graner A, Sorrells ME (2005a) Genic Microsatellite Markers: Features and Applications. Trends Biotechnol 23: 48-55 crossref(new window)

19.
Varshney RK, Sigmund R, Borner A, Korzun V, Stein N, Sorrells ME, Langridge P, Graner A (2005b) Interspecific Transferability and Comparative Mapping of Barley EST-SSR Markers in Wheatrye and Rice. Plant Sci 168:195-202 crossref(new window)

20.
Wang ML, Barkely NA, Jenkins TM (2009) Microsatellite Markers in Plants and Insects. Part I: Applications of Biotechnology. Genes Genom Gen 3:54-67

21.
Yamazaki M, Sato A, Shimomura K, Saito K, Murakoshi I (1994) Genetic Relationships among Glycyrrhiza Plants Determined by RAPD and RFLP Analyses. Biol Pharm Bull 17:1529-1531 crossref(new window)

22.
Yao H, Zhao Y, Chen DF, Chen JK, Zhao TS (2008) ISSR Primer Screening and Preliminary Evaluation of Genetic Diversity in Wild Populations of Glycyrrhiza uralensis. Biol Plantarum 52:117-120 crossref(new window)

23.
Zhang G, Sun Z, Zhou D, Xiong M, Wang X, Yang J, Wei Z (2015) Development and Characterization of Novel EST-SSRs from Larix gmelinii and Their Cross-Species Transferability. Molecules 20:12469-12480 crossref(new window)

24.
Zhang Q, Ye M (2009) Chemical Analysis of the Chinese Herbal Medicine Gan-Cao (licorice). J Chromatogr A 1216:1954- 1569 crossref(new window)