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Rapid metabolic discrimination between Zoysia japonica and Zoysia sinica based on multivariate analysis of FT-IR spectroscopy
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  • Journal title : Journal of Plant Biotechnology
  • Volume 43, Issue 2,  2016, pp.213-222
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2016.43.2.213
 Title & Authors
Rapid metabolic discrimination between Zoysia japonica and Zoysia sinica based on multivariate analysis of FT-IR spectroscopy
Yang, Dae-Hwa; Ahn, Myung Suk; Jeong, Ok-Cheol; Song, In-Ja; Ko, Suk-Min; Jeon, Ye-In; Kang, Hong-Gyu; Sun, Hyeon-Jin; Kwon, Yong-Ik; Kim, Suk Weon; Lee, Hyo-Yeon;
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This study aims to establish a system for the rapid discrimination of Zoysia species using metabolite fingerprinting of FT-IR spectroscopy combined with multivariate analysis. Whole cell extracts from leaves of 19 identified Zoysia japonica, 6 identified Zoysia sinica, and 38 different unidentified Zoysia species were subjected to Fourier transform infrared spectroscopy (FT-IR). PCA (principle component analysis) and PLS-DA (partial least square discriminant analysis) from FT-IR spectral data successfully divided the 25 identified turf grasses into two groups, representing good agreement with species identification using molecular markers. PC (principal component) loading values show that the region of the FT-IR spectra are important for the discrimination of Zoysia species. A dendrogram based on hierarchical clustering analysis (HCA) from the PCA and PLS-DA data of turf grasses showed that turf grass samples were divided into Zoysia japonica and Zoysia sinica in a species-dependent manner. PCA and PLS-DA from FT-IR spectral data of Zoysia species identified and unidentified by molecular markers successfully divided the 49 turf grasses into Z. japonica and Z. sinica. In particular, PLS-DA and the HCA dendrogram could mostly discriminate the 47 Z. japonica grasses into two groups depending on their origins (mountainous areas and island area). Considering these results, we suggest that FT-IR fingerprinting combined with multivariate analysis could be applied to discriminate between Zoysia species as well as their geographical origins of various Zoysia species.
Turf grasses;Zoysia japonica;Zoysia sinica;Fourier transform - infrared spectroscopy (FT-IR);Principal component analysis (PCA);Partial least square discriminant analysis (PLS-DA);
 Cited by
Ahn MS, Min SR, Jie EY, So EJ, Choi SY, Moon BC, Kang YM, Park SY, Kim SW (2015) Rapid comparison of metabolic equivalence of standard medicinal parts from medicinal plants and their in vitro-generated adventitious roots using FT-IR spectroscopy. J Plant Biotechnol 42:257-264 crossref(new window)

Christians N (1998) Fundamentals of turfgrass management, Ann Arbor Press, Chelsea, MI.

Chung SJ, Park SJ, Choi YI, Kim IK, Lee KY, Kim HJ, Lee GJ (2013) SCAR markers were developed to identify zoysiagrass mutants exhibiting fine leaf characteristics. CNU J Agr Sci 40:1-5

D'Souza L, Devi P, Shridhar MPD and Naik CG. 2008. Use of Fourier Transform Infrared (FTIR) Spectroscopy to Study Cadmium-Induced Changes in PadinaTetrastromatica (Hauck). Anal Chem Insights 3:135-143

Dumas P, Miller LM (2003) The use of synchrotron infrared microspectroscopy in biological and biomedical investigations. Vib Spectrosc 32:3-21 crossref(new window)

Fiehn O, Kopka J, Drmann P, Altmann T, Trethewey R, Willmitzer L (2000) Metabolite profiling for plant functional genomics. Nat Biotechnol 18:1157-1161 crossref(new window)

Hyun YH, Choi BJ, Kim YJ, Joo YK (2012) Analysis of Research Trend on Zoysiagrass (Zoysia spp.) Asian J. Turfgrass Sci 26(2):89-95

Kang BC, Namkung Y, Shin HK (1999) Analysis of the genetic variation in Anyang Joonggi and development of Anyang Joonggi specific DNA marker. Kor J Hort Sci Technol 17:197

Kim SW, Ban SH, Chung H, Cho SH, Chung HJ, Choi PS, Yoo OJ, Liu JR (2004) Taxonomic discrimination of higher plants by multivariate analysis of Fourier transform infrared spectroscopy data. Plant Cell Rep 23:246-250 crossref(new window)

Kim SW, Cho SH, Chung H, Liu JR (2007) Genetic discrimination between Catharanthus roseus cultivars by multivariate analysis of fourier transform infrared spectroscopy data. J Plant Biotechnol 34:201-205 crossref(new window)

Kim SW, Min SR, Kim JH, Park SK, Kim TI, Liu JR (2009) Rapid discrimination of commercial strawberry cultivars using Fourier transform infrared spectroscopy data combined by multivariate analysis. Plant Biotechnol Rep 3:87-93 crossref(new window)

Krishnan P, Kruger NJ, Ratcliffe RG (2005) Metabolite fingerprinting and profiling in plants using NMR. J Exp Bot 56:255-265

Kwon YK, Kim SW, Seo JM, Woo TH, Liu JR (2011) Prediction and discrimination of taxonomic relationship within Orostachys species using FT-IR spectroscopy combined by multivariate analysis. J Plant Biotechnol 38:9-14 crossref(new window)

Kwon YK, Ahn MS, Park JS, Liu JR, In DS, Min BW, Kim SW (2014) Discrimination of cultivation ages and cultivars of ginseng leaves using Fourier transform infrared spectroscopy combined with multivariate analysis. J Ginseng Res 38:52-58 crossref(new window)

Li, RF, Wei JH, Wang HZ, He J, Sun ZY (2006) Development of highly regenerable callus lines and Agrobacterium-mediated transformation of Chinese lawngrass (Zoysia sinica Hance) with a cold inducible transcription factor, CBF1. Plant Cell Tiss Org 85:297-305 crossref(new window)

Lopez-Sanchez M, Ayora-Canada MJ, Molina-Diaz A (2010) Olive fruit growth and ripening as seen by vibrational spectroscopy. J Agric Food Chem 58:82-87 crossref(new window)

Parker FS. 1983. Applications of infrared, raman and resonance raman spectroscopy in biochemistry, Plenum Press, New York, P. 527

Sun HJ, Song IJ, Bae TW, Lee HY (2010) Recent development in biotechnological improvement of Zoysia japonica Steud. J Plant Biotechnol 37:400-407 crossref(new window)

Song SY, Ha TJ, Jang KC, Kim IJ, Kim SW (2012) Establishment of rapid discrimination system of leguminous plants at metabolic level using FT-IR spectroscopy with multivariate analysis. J Plant Biotechnol 39:121-126 crossref(new window)

Song SY, Jie EY, Ahn MS, Lee IH, Nou IS, Min BW, Kim SW (2014) Fourier Transform Infrared (FT-IR) spectroscopy of genomic DNA to discriminate F1 progenies from their paternal lineage of Chinese cabbage (Brassica rapa subsp. pekinensis). Mol Breeding 33:453-464 crossref(new window)

Trygg J, Holmes E, Londstedt T (2007) Chemometrics in metabonomics. J Proteome Res 6:467-479

Turgeon AJ. 1985. Turgrass Management, Rev Ed, Raston Publishing, Raston, Virginia

Wolkers WF, Oliver AE, Tablin F, Crowe JH (2004) A fourier transform infrared spectroscopy study of sugar glasses. Carb Res 339:1077-85 crossref(new window)

Yang GM, Ahn BJ, Choi JS (1995) Identification of native zoysiagrasses (Zoysia spp.) using morphological characteristics and esterase isozymes. J Kor Soc Hort Sci 36(2):240-247

Yee N, Benning LG, Phoenix VR, Ferris FG (2004) Characterization of metal-Cyanobacteria sorption reactions: A combined macroscopic and infrared spectroscopic investigation. Environ Sci Technol 38:775-82 crossref(new window)

Yu TY, Yeam DY, Kim, YJ, Kim SJ (1974) Morphological studies on Korean lawn grasses (Zoysia spp.). Jour Kor Soc Hort Sci 15(1):79-91