Advanced SearchSearch Tips
DNA microarray analysis of RNAi plant regulated expression of NtROS2a gene encoding cytosine DNA demethylation
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Plant Biotechnology
  • Volume 43, Issue 2,  2016, pp.231-239
  • Publisher : The Korean Society of Plant Biotechnology
  • DOI : 10.5010/JPB.2016.43.2.231
 Title & Authors
DNA microarray analysis of RNAi plant regulated expression of NtROS2a gene encoding cytosine DNA demethylation
Choi, Jang Sun; Lee, In Hye; Jung, Yu Jin; Kang, Kwon Kyoo;
  PDF(new window)
To study the transcript levels of epigenetically regulated genes in tobacco, we have developed a transgenic line OX1 overexpressing NtROS2a gene encoding cytosine DNA demethylation and a RNAi plant line RNAi13. It has been reported that salt- and -stress tolerance of these transgenic lines are enhanced with various phenotypic characters (Lee et al. 2015). In this paper, we conducted microarray analysis with Agilent Tobacco 4 x 44K oligo chip by using overexpression line OX1, RNAi plant line RNAi 13, and wild type plant WT. Differentially expressed genes (DEGs) related to metabolism, nutrient supply, and various stressed were up-regulated by approximately 1.5- to 80- fold. DEGs related to co-enzymes, metabolism, and methylation functional genes were down-regulated by approximately 0.03- to 0.7- fold. qRT-PCR analysis showed that the transcript levels of several candidate genes in OX1 and RNAi lines were significantly (p < 0.05) higher than those in WT, such as genes encoding KH domain-containing protein, MADS-box protein, and Zinc phosphodiesterase ELAC protein. On the other hand, several genes such as those encoding pentatricopeptide (PPR) repeat-containing protein, histone deacetylase HDAC3 protein, and protein kinase were decreased by approximately 0.4- to 1.0- fold. This study showed that NtROS2a gene encoding DNA glycosylase related to demethylation could regulate adaptive response of tobacco at transcriptional level.
DNA demethylation;methylation;microarray analysis;NtROS2a transgenic tobacco;transcriptional level;
 Cited by
시토신 탈메틸화 관련 NtROS2a 유전자 도입 형질전환벼의 건조스트레스 내성 증진,최장선;이인혜;조용구;정유진;강권규;

Journal of Plant Biotechnology, 2016. vol.43. 3, pp.376-382 crossref(new window)
Overexpression ofNtROS2agene encoding cytosine DNA demethylation enhances drought tolerance in transgenic rice, Journal of Plant Biotechnology, 2016, 43, 3, 376  crossref(new windwow)
Amasino R. 2010. Seasonal and developmental timing of flowering. Plant J 61:1001-1013 crossref(new window)

Bird AP. 2002. DNA methylation patterns and epigenetic memory. Genes Dev. 16:6-21 crossref(new window)

Boyko A, Kovalchuk I. 2010. Transgenerational response to stress in Arabidopsis thaliana. Plant Signal Behav 5:995-998 crossref(new window)

Gao MJ, Schafer UA, Parkin IA, Hegedus DD, Lydiate DJ, Hannoufa A. 2003. A novel protein from Brassica napus has a putative KID domain and responds to low temperature. Plant J 33:1073-1086 crossref(new window)

Choi CS, Hiroshi Sano. 2007. Abiotic-stress induces demethylation and transcriptional activation of a gene encoding a glycerophosphodiesterase- like protein in tobacco plants. Mol. Genet. Genomics 277:589-00 crossref(new window)

Feng S, Jacobsen SE, Reik W. 2010. Epigenetic reprogramming in plant and animal development. Science 330:622-627 crossref(new window)

Gehring M, Huh JH, Hsieh TF, Penterman J, Choi Y, Harada JJ, Goldberg R, Fischer RL. 2006. DEMETER DNA glycosylase establishes MEDEA polycomb gene self-imprinting by allelespecific demethylation. Cell 124:495-506 crossref(new window)

Goldberg AD, Allis CD, Bernstein E. 2007. Epigenetics: a landscape takes shape. Cell 128:635-638 crossref(new window)

Heo JB, Sung S. 2011. Vernalization-mediated epigenetic silencing by a long intronic noncoding RNA. Science 331:76-79 crossref(new window)

Huh JH, Bauer MJ, Hsieh TF, Fischer RL. 2008. Cellular programming of plant gene imprinting. Cell 132:735-744 crossref(new window)

Kim DH, Doyle MR, Sung S, Amasino RM. 2009. Vernalization: winter and the timing of flowering in plants. Annu Rev Cell Dev Biol 25:277-299 crossref(new window)

Law JA, Jacobsen SE. 2010. Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat. Rev. Genet. 11: 204-220 crossref(new window)

Lee IH, Choi JS, Marjohn N, Cho YG, Kang KK, Jung YJ. 2015. Regulation of abiotic stress response through NtROS2a-mediated demethylation in tobacco. Plant Breed. Biotech. 3(2):108-118 crossref(new window)

Li E, Bestor TH, Jaenisch R. 1992. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69: 915-926. crossref(new window)

Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the $^{2-{\Delta}{\Delta}CT}$ method. Methods 25:402-408 crossref(new window)

Morales-Ruiz T, Ortega-Galisteo AP, Ponferrada-Marin MI, Martinez-Macias MI, Ariza RR, Roldan-Arjona T. 2006. DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases. Proc. Natl. Acad. Sci. USA 103:6853-6858 crossref(new window)

Reik W, Dean W, Walter J. 2001. Epigenetic reprogramming in mammalian development. Science 293:1089-1093 crossref(new window)

Sano H, Kamada I, Youssefian S, Katsumi M, Wabiko H. 1990. Asingle treatment of rice seedlings with 5-azacytidine induces heritable dwarfism and under methylation of genomic DNA. Molecular Genetics and Genomics 220:441-447 crossref(new window)

Steward N, Ito M, Yamakuchi Y, Koizumi N, Sano H. 2002. Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. J. Biol. Chem. 277: 37741-37746 crossref(new window)

Wu SC, Zhang Y. 2010. Active DNA demethylation: many roads lead to Rome. Nat Rev Mol Cell Biol. 11:607-620

Zhang X, Yazaki J, Sundaresan A, Cokus S, Chan SW, Chen H, Henderson IR, Shinn P, Pellegrini M, Jacobsen SE et al. 2006. Genome-Wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell 126:1189-1201 crossref(new window)

Zhu J, Jeong JC, Zhu Y, Sokolchik I, Miyazaki S, Zhu JK, Hasegawa PM, Bohnert HJ, Shi H, Yun DJ, Bressan RA. 2008. Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance. Proc. Natl. Acad. Sci. USA 105:4945-4950 crossref(new window)