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PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN
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 Title & Authors
PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN
Kim, Seon-Mi; Choi, Nam-Ki; Cho, Seong-Hoon; Lee, Seok-Woo; Lim, Hoi-Jeong; Lim, Hoi-Soon; Kang, Mi-Sun; Oh, Jong-Suk;
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 Abstract
The purpose of this study was to assess the prevalence of periodontopathic bacteria and resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M), ermF, aacA-aphD, cfxA, , , vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia. and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition. the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants. cfxA, and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance. associated with resistance to -lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and . Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).
 Keywords
Periodontopathic bacteria;Antibiotic resistance;Antibiotic resistance determinants;Oral biofilm;Polymerase chain reaction;
 Language
Korean
 Cited by
1.
감귤에서 분리한 정유 성분의 항균활성 연구,정시화;김선빈;최학주;김동희;

대전대학교 한의학연구소 논문집, 2012. vol.20. 2, pp.67-78
1.
The Interactive Effect of These Bacterial Substrates on the Growth of Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis, Journal of dental hygiene science, 2015, 15, 2, 209  crossref(new windwow)
2.
Prevalence of Periodontopathogens in Saliva and Plaque of Korean Children and Adolescents, THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, 2016, 43, 1, 8  crossref(new windwow)
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