JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Diagnosis of Early Dental Caries with Dye-Enhancing Quantitative Light-Induced Fluorescence (QLF)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Diagnosis of Early Dental Caries with Dye-Enhancing Quantitative Light-Induced Fluorescence (QLF)
Kim, Mihee; Lee, Sangho; Lee, Nanyoung;
  PDF(new window)
 Abstract
This study used sodium fluorescein to improve imaging diagnostic ability by increasing the fluorescence difference between sound enamel and caries lesions. It also made it easier to discriminate between stain and caries lesions using quantitative light-induced fluorescence (QLF). Half of the specimen surface was covered with nail varnish as a control. Specimens were divided randomly in six decalcification groups and decalcified for different lengths of time. Then, was measured using QLF-D. After applying 0.075% sodium fluorescein, we measured again and compared it with the initial value. After cutting the central portion of the specimen, we measured the lesion depth using scanning electron microscopy. The lesion surfaces observed with QLF were darker than normal enamel, whereas they were lighter than normal enamel after applying fluorescein. Longer decalcification time was associated with greater fluorescent dye penetration. The measured after applying fluorescein was higher than the initial value (p < 0.05). Due to QLF measurement using fluorescein being more sensitive for diagnosing early decalcification, this approach will enable early diagnosis of dental caries before the cavity formation stage, allowing the treatment of early caries lesions. With QLF and sodium fluorescein, we can easily discriminate between stain and caries lesions.
 Keywords
Dental caries;Quantitative light-induced fluorescence;Sodium fluorescein;
 Language
Korean
 Cited by
 References
1.
Lee SH : Present situation and prospect of pediatric dentistry in Korea - Focused on management of dental caries -. J Korean Acad Pediatr Dent, 39:206-225, 2012. crossref(new window)

2.
Wefel JS, Harless JD : Comparison of artificial white spots by microradiography and polarized light microscopy. J Dent Res, 63:1271-1275, 1984. crossref(new window)

3.
Ekstrand K, Qvist V, Thylstrup A : Light microscope study of the effect of probing in occlusal surfaces. Caries Res, 21:368-374, 1987. crossref(new window)

4.
van Dorp CS, Exterkate RA, ten Cate JM : The effect of dental probing on subsequent enamel demineralization. ASDC J Dent Child, 55:343-347, 1988.

5.
Lussi A : Validity of diagnostic and treatment decisions of fissure caries. Caries Res, 25:296-303, 1991. crossref(new window)

6.
Penning C, van Amerongen JP, Seef RE, ten Cate JM : Validity of probing for fissure caries diagnosis. Caries Res, 26:445-449, 1992. crossref(new window)

7.
Warren JJ, Levy SM, Wefel JS : Explorer probing of root caries lesions: an in vitro study. Spec Care Dentist, 23:18-21, 2003. crossref(new window)

8.
Akarsu S, Koprulu H : In vitro comparison of the efficacy of DIAGNOdent by visual inspection and radiographic diagnostic techniques in the diagnosis of occlusal caries. J Clin Dent, 17:53-58, 2006.

9.
Costa AM, Bezzerra AC, Fuks AB : Assessment of the accuracy of visual examination, bite-wing radiographs and DIAGNOdent on the diagnosis of occlusl acries. Eur Arch Paediatr Dent, 8:118-122, 2007. crossref(new window)

10.
Sridhar N, Tandon S, Rao N : A comparative evaluation of DIAGNOdent with visual and radiography for detection of occlusal caries: an in vitro study. Indian J Dent Res, 20:326-331, 2009. crossref(new window)

11.
Stookey GK : Quantitative light fluorescence: a technology for early monitorig of the caries process. Dent Clin North Am, 49:753-770, 2005. crossref(new window)

12.
Hwang KS, Kim JS, Yoo SH : Comparative study on the efficacy of Digital Imaging Fiber-Optic Trans-Illumination and Laser Fluorescence in monitoring the remineralization process of incipient smooth surface enamel lesions. J Korean Acad Pediatr Dent, 34:183-191, 2007.

13.
Yanikoglu F, Ozturk F, Stookey GK, et al. : Detection of natural white spot caries lesions by an ultrasonic system. Caries Res, 34:225-232, 2000. crossref(new window)

14.
Kim HS, Kim WK, Lee CS, Lee SH : Optical sensitivity of laser fluorescence for incipient caries detection. J Korean Acad Pediatr Dent, 26:109-118, 1999.

15.
Maeng MH, Kim JS, Kim SO : Comparative study on the early detection of enamel lesions using DIFOTI and laser fluorescence. J Korean Acad Pediatr Dent, 33:207-220, 2006.

16.
Adeyemi AA, Jarad FD, Pender N, Higham SM : Comparison of quantitative light-induced fluorescence (QLF) and digital imaging applied for the detection and quantification of staining and stain removal on teeth. J Dent, 34:460-466, 2006 crossref(new window)

17.
al-Sehaibany F, White G, Rainey JT : The use of caries detector dye in diagnosis of occlusal caries lesions. J Clin Pediatr Dent, 20:293-298, 1996.

18.
Van de Rijke JW, Ten Bosch JJ : Optical quantification of caries-like lesion in vitro by use of a fluorescent dye. J Dent Res, 69:1184-1187, 1990. crossref(new window)

19.
Van de Rijke JW : Use of dyes in cariology. Int Dent J, 41:111-116, 1991.

20.
Eggertsson H, Analoui M, Stookey G, et al. : Detection of early proximal caries in vitro using laser fluorescence, dye-enhanced laser fluorescence and direct visual examination. Caries Res, 33:227-233, 1999. crossref(new window)

21.
Vaarkamp J, ten Bosch J, Verdonschot EH, Huysmans MC : Wavelength-dependent fiber-optic transillumination of small approximal caries lesions: The use of a dye, and a comparison to bitewing radiography. Caries Res, 31:232-237, 1997. crossref(new window)

22.
Arends J, ten Bosch JJ : Demineralization and remineralization evaluation techniques. J Dent Res, 71 Spec No:924-928, 1992.

23.
Reynolds EC : Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions. J Dent Res, 76:1587-1595, 1997. crossref(new window)

24.
Benson PE, Pender N, Higham SM : An in situ caries model to study demineralization during fixed orthodontics. Clin Orthod Res, 2:143-153, 1999.

25.
Ogaard B, Rola G : The in vivo orthodontic banding model for vital teeth and the in situ orthodontic banding model for hard-tissue slabs. J Dent Res, 71 Spec No:832-835, 1992.

26.
Fontana M, Li Y, Stookey GK, et al. : Measurement of enamel demineralization using microradiography and confocal microscopy. A correlation study. Caries Res, 30:317-325, 1996. crossref(new window)

27.
Aljehani A, Tranaeus S, Shi XQ, et al. : In vitro quantification of white spot enamel lesions adjacent to fixed orthodontic appliances using quantitative light-induced fluorescence and DIAGONOdent. Acta Odontol Scand, 62:313-318, 2004. crossref(new window)

28.
Kühnisch J, Ifland S, Heinrich-Weltzien R : In vivo detection of non-cavitated caries lesions on occlusal surfaces by visual inspection and quantitative lightinduced flurescence. Acta Odontol Scand, 65:183-188, 2007. crossref(new window)

29.
Alammari MR, Smith PW, de Josselin de Jong E, Higham SM : Quantitative light-induced fluorescence:a tool for early occlusal dental caries detection and supporting decision making in vivo. J Dent, 41:127-132, 2013. crossref(new window)

30.
Kim HE, Kwon HK, Kim BI : Recovery percentage of remineralization according to severity of early caries. Am J Dent, 26:132-136, 2013.

31.
Sagel PA, Lapujade PG, Miller JM, Sunberg RJ : Objective quantification of plaque using digital image analysis. Monogr Oral Sci, 17:130-143, 2000. crossref(new window)

32.
Lund F, Jogestrand T, Kolegard R : Computerized analysis of video fluorescein imaging of the skin. Clin Physiol, 20:374-379, 2000. crossref(new window)

33.
Rosen B : Fluorescein angiography in laser treatment of diabetic macular edema. Ophthalmology, 108:237-239, 2001.

34.
Mormann W, Regolati B, Lutz F, Saxer UP : The gingivitis fluorescein test in recruits. Helv Odontol Acta, 19:27-30, 1975.

35.
Van der Veen MH, Tsuda H, Arends J, ten Bosch JJ : Evaluation of sodium fluorescein for quantitative diagnosis of root caries. J Dent Res, 75:588-593, 1996. crossref(new window)

36.
Van der Veen MH, ten Bosch JJ : An in vitro evaluation of fluorescein penetration into natural root surface carious lesions. Caries Res, 27:258-261, 1993. crossref(new window)

37.
Lee SH, Han KJ, Lee CS : Diagnosis of early caries with dye-enhanced laser fluorescence. J Korean Acad Pediatr Dent, 30;1-9, 2003.

38.
Pretty IA, Ingram GS, Higram SM, et al. : The use of fluorescein-enhanced quantitative light-induced fluorescence to monitor de- and re- mineralization of in vitro root caries. J Oral Rehabil, 30:1151-1156, 2003. crossref(new window)