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Antibacterial Properties of Pit and Fissure Sealant Containing S-PRG filler on Streptococcus mutans
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 Title & Authors
Antibacterial Properties of Pit and Fissure Sealant Containing S-PRG filler on Streptococcus mutans
An, Jinseon; Park, Howon; Seo, Hyunwoo; Lee, Siyoung;
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The purpose of this study was to evaluate the antibacterial properties of a sealant containing S-PRG filler compared to those of two contemporary commercial sealants to determine the inhibition of bacterial growth in broth culture and biofilm formation using the CDC Biofilm Reactor. The BeautiSealant containing S-PRG filler, the fluoride releasing Clinpro sealant, which are known to have higher antibacterial effects, and the non-fluoride releasing Concise sealant were selected for this study. A Streptococcus mutans culture in BHI broth without sealant served as a negative control in the planktonic growth inhibition test. As a result, bacterial growth was inhibited in all three sealant groups compared to that in the control. The Clinpro sealant showed a significantly reduced number of CFUs compared to those of the BeautiSealant and Concise sealants. However, no significant difference was detected between the BeautiSealant and Concise sealants. The Clinpro sealant significantly decreased biofilm formation compared to that by the BeautiSealant and Concise sealants. No significant difference was observed between the BeautiSealant and Concise sealants. In conclusion, the sealant containing S-PRG filler had a less potent anti-bacterial property and increased biofilm formation capacity compared to those of the fluoride releasing Clinpro sealant.
S-PRG filler;BeautiSealant;Pit and fissure sealants;Streptococcus mutans;Fluoride;
 Cited by
Comparison of Cariogenicity of Bovine Milk and Low-fat Milk on Streptococcus mutans Biofilm, THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, 2017, 44, 2, 170  crossref(new windwow)
Griffin SO, Gray SK, Malvitz DM, Gooch BF : Caries risk in formerly sealed teeth. J Am Dent Assoc, 140:415-423, 2009. crossref(new window)

Ahovuo-Saloranta A, Forss H, Walsh T, et al. : Sealants for preventing dental decay in the permanent teeth. Cochrane Database Syst Rev, 3: Cd001830, 2013.

Mejare I, Lingstrom P, Petersson LG, et al. : Caries-preventive effect of fissure sealants: a systematic review. Acta Odontol Scand, 61:321-330, 2003. crossref(new window)

Matalon S, Peretz B, Sidon R, et al. : Antibacterial properties of pit and fissure sealants combined with daily fluoride mouth rinse. Pediatr Dent, 32:9-13, 2010.

Loyola-Rodriguez JP, Garcia-Godoy F : Antibacterial activity of fluoride release sealants on mutans streptococci. J Clin Pediatr Dent, 20:109-111, 1996.

Petrovski KR, Caicedo-Caldas A, Williamson NB, et al. : Efficacy of a novel internal dry period teat sealant containing 0.5% chlorhexidine against experimental challenge with Streptococcus uberis in dairy cattle. J Dairy Sci, 94:3366-3375, 2011. crossref(new window)

Yang SY, Piao YZ, Kim SM, et al. : Acid neutralizing, mechanical and physical properties of pit and fissure sealants containing melt-derived 45S5 bioactive glass. Dent Mater, 29:1228-1235, 2013. crossref(new window)

Al-Naimi, Al-Alousi, Al-Nema : Effect of silver and zinc oxide nanoparticle addition on microhardness and depth of cure of resin based pit and fissure sealants. IJERSTE, 4:157-163, 2015.

Naorungroj S, Wei HH, Arnold RR, et al. : Antibacterial surface properties of fluoride-containing resin-based sealants. J Dent, 38:387-391, 2010. crossref(new window)

Shin SW, Kim JS : Microleakage and anticariogenic effect of S-PRG filler-containing pit and fissure sealant. J Korean Acad Pediatr Dent, 40:247-252, 2013. crossref(new window)

Tay FR, Sano H, Tagami J, et al. : Ultrastructural study of a glass ionomer-based, all-in-one adhesive. J Dent, 29:489-498, 2001. crossref(new window)

Ikemura K, Tay FR, Kouro Y, et al. : Optimizing filler content in an adhesive system containing prereacted glass-ionomer fillers. Dent Mater, 19:137- 146, 2003. crossref(new window)

Ikemura K, Tay FR, Endo T, Pashley DH : A review of chemical-approach and ultramorphological studies on the development of fluoride-releasing dental adhesives comprising new pre-reacted glass ionomer (PRG) fillers. Dent Mater J, 27:315-339, 2008. crossref(new window)

Kamijo K, Mukai Y, Tominaga T, et al. : Fluoride release and recharge characteristics of denture base resins containing surface pre-reacted glass-ionomer filler. Dent Mater J, 28:227-233, 2009. crossref(new window)

Mukai Y, Kamijo K, Fujino F, et al. : Effect of denture base-resin with prereacted glass-ionomer filler on dentin demineralization. Eur J Oral Sci, 117: 750-754, 2009. crossref(new window)

Tomiyama K, Mukai Y, Teranaka T : Acid resistance induced by a new orthodontic bonding system in vitro. Dent Mater J, 27:590-597, 2008. crossref(new window)

Saku S, Kotake H, Scougall-Vilchis RJ, et al. : Antibacterial activity of composite resin with glassionomer filler particles. Dent Mater J, 29:193-198, 2010. crossref(new window)

Yoneda M, Suzuki N, Masuo Y, et al. : Effect of SPRG eluate on biofilm formation and enzyme activity of oral bacteria. Int J Dent, 2012:814913, 2012.

Ying W, Masayuki K, Daisuke K : Ion release and buffering capacity of S-PRG filler-containing pit and fissure sealant in lactic acid. Nano Biomedicine, 3: 275-281, 2011.

Kimyai S, Lotfipour F, Pourabbas R, et al. : Effect of two prophylaxis methods on adherence of Streptococcus mutans to microfilled composite resin and giomer surfaces. Med Oral Patol Oral Cir Bucal, 16:e561-567, 2011.

Goeres DM, Loetterle LR, Hamilton MA, et al. : Statistical assessment of a laboratory method for growing biofilms. Microbiology, 151:757-762, 2005. crossref(new window)

Ogawa A, Furukawa S, Fujita S, et al. : Inhibition of Streptococcus mutans biofilm formation by Streptococcus salivarius FruA. Appl Environ Microbiol, 77:1572-1580, 2011. crossref(new window)

Oong EM, Griffin SO, Kohn WG, et al. : The effect of dental sealants on bacteria levels in caries lesions: a review of the evidence. J Am Dent Assoc, 139: 271-278, 2008. crossref(new window)

Song KW : Study on the degree of conversion and fluoride release of fluoride-containing sealants. Ph. D. Dissertation, Seoul University, Seoul, 1999.

Seo YJ : Effect of resin monomers on the activity of cariogenic bacteria. Ph. D. Dissertation, Chosun University, Kwang Ju, 2004.

Itota T, Carrick TE, Yoshiyama M, McCabe JF : Fluoride release and recharge in giomer, compomer and resin composite. Dent Mater, 20:789-795, 2004. crossref(new window)

Wiegand A, Buchalla W, Attin T : Review on fluoride- releasing restorative materials-fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater, 23:343- 362, 2007. crossref(new window)

Van Loveren C : Antimicrobial activity of fluoride and its in vivo importance: identification of research questions. Caries Res, 1:65-70, 2001.

Guida A, Towler MR, Wall JG, et al. : Preliminary work on the antibacterial effect of strontium in glass ionomer cements. J Mater Sci Lett, 22:1401-1403, 2003. crossref(new window)

Dabsie F, Gregoire G, Sixou M, Sharrock P : Does strontium play a role in the cariostatic activity of glass ionomer:Strontium diffusion and antibacterial activity. J Dent, 37:554-559, 2009. crossref(new window)

Koletsi-Kounari H, Mamai-Homata E, Diamanti I : An in vitro study of the effect of aluminum and the combined effect of strontium, aluminum, and fluoride elements on early enamel carious lesions. Biol Trace Elem Res, 147:418-427, 2012. crossref(new window)

Dembitsky VM, Al Quntar AA, Srebnik M : Natural and synthetic small boron-containing molecules as potential inhibitors of bacterial and fungal quorum sensing. Chem Rev, 111:209-237, 2011. crossref(new window)

Kaga M, Kakuda S, Ida Y, et al. : Inhibition of enamel demineralization by buffering effect of SPRG filler-containing dental sealant. Eur J Oral Sci, 122:78-83, 2014. crossref(new window)

Suzuki N, Yoneda M, Haruna K, et al. : Effects of S-PRG eluate on oral biofilm and oral malodor. Arch Oral Biol, 59:407-413, 2014. crossref(new window)

Hahnel S, Wastl DS, Schneider-Feyrer S, et al. : Streptococcus mutans biofilm formation and release of fluoride from experimental resin-based composites depending on surface treatment and S-PRG filler particle fraction. J Adhes Dent, 16:313-321, 2014.

Fujimoto Y, Iwasa M, Murayama R, et al. : Detection of ions released from S-PRG fillers and their modulation effect. Dent Mater J, 29:392-397, 2010. crossref(new window)

Ito S, Iijima M, Hashimoto M, et al. : Effects of surface pre-reacted glass-ionomer fillers on mineral induction by phosphoprotein. J Dent, 39:72-79, 2011. crossref(new window)

Al-Bakri IA, Harty D, Al-Omari WM, et al. : Surface characteristics and microbial adherence ability of modified polymethylmethacrylate by fluoridated glass fillers. Aust Dent J, 59:482-489, 2014. crossref(new window)

Fan Y, Townsend J, Wang Y, et al. : Formulation and characterization of antibacterial fluoride-releasing sealants. Pediatr Dent, 35:E13-18, 2013.

Costerton JW, Stewart PS, Greenberg EP : Bacterial biofilms: a common cause of persistent infections. Science, 284:1318-1322, 1999. crossref(new window)

Brambilla E, Ionescu A, Cazzaniga G, et al. : The influence of antibacterial toothpastes on in vitro Streptococcus mutans biofilm formation: a continuous culture study. Am J Dent, 27:160-166, 2014.

Somanah J, Bourdon E, Bahorun T, Aruoma OI : The inhibitory effect of a fermented papaya preparation on growth, hydrophobicity, and acid production of Streptococcus mutans, Streptococcus mitis, and Lactobacillus acidophilus: its implications in oral health improvement of diabetics. Food science & nutrition, 1:416-421, 2013. crossref(new window)

Ikeda M, Matin K, Nikaido T, et al. : Effect of surface characteristics on adherence of S. mutans biofilms to indirect resin composites. Dent Mater J, 26:915-923, 2007. crossref(new window)

Hannig M : Transmission electron microscopy of early plaque formation on dental materials in vivo. Eur J Oral Sci, 107:55-64, 1999. crossref(new window)

Hotta M, Morikawa T, Tamura D, Kusakabe S : Adherence of Streptococcus sanguinis and Streptococcus mutans to saliva-coated S-PRG resin blocks. Dent Mater J, 33:261-267, 2014. crossref(new window)