Advanced SearchSearch Tips
Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Characterization of Differentiation of the Supernumerary Dental Pulp Stem Cells toward the Odontoblast by Application Period of Additives
Kim, Jongsoo;
  PDF(new window)
The aim of this study was to investigate the possibility of the supernumerary teeth for the stem cell source in dentistry. The Real Time Quantitative Reverse Transcription Polymerase Chain Reaction (Real Time qRT-PCR) method was used to evaluate the differentiation toward the odontoblast of the supernumerary dental pulp stem cells (sDPSCs). Supernumerary dental pulp stem cells were obtained from 3 children (2 males and 1 female, age 7 to 9) diagnosed that the eruption of permanent teeth was disturbed by supernumerary teeth. The common genes for odontoblasts are alkaline phosphatase (ALP), osteocalcin (OC), osteonectin (ON), dentin matrix acidic phosphoprotein 1 (DMP-1), dentin sialophosphoprotein (DSPP). The sDPSCs were treated for 0 days, 8 days and 14 days with additives and then Real Time qRT-PCR was performed in intervals of 0 days, 8 days and 14 days. The alizarin-red solution staining was performed to visualize the stained color for the degree of calcification at 7 days, 14 days, 21 days and 28 days after treating additives to the sDPSCs. From the result of the Real Time qRT-PCR, the manifestation exhibit maximum value at 8 days after additive treatment and shifted to a decrease trend at 14 days. Alizarin-red solution staining exhibit light results at 7 days after staining and generalized dark result at 14 days. Consequently, in studies with sDPSCs, appropriate treatment time of additives for Real Time qRT-PCR is 8 days. Also, a suitable period of Alizarin-red solution staining is 14 days.
Odontoblast;Supernumerary Tooth;Additives;Stem Cell;
 Cited by
Aubin J, Liu F, Malaval L, et al. : Osteoblast and chondroblast differentiation. Bone, 17:S77-S83, 1995. crossref(new window)

Arana-Chavez VE, Massa LF : Odontoblasts: the cells forming and maintaining dentine. Int J Biochem Cell Biol, 36:1367-1373, 2004. crossref(new window)

Park JW, Song JS, Son HG, et al. : Effect of storage media and duration on pulpal cell viability in exfoliated deciduous teeth. J Korean Acad Pediatr Dent, 41:1-7, 2014. crossref(new window)

Gronthos S, Brahim J, Li W, et al. : Stem cell properties of human dental pulp stem cells. J Dent Res, 81:531-535, 2002. crossref(new window)

Song JS, Kim SH, Jung HS, et al. : Characterization of Stem Cells Obtained from the Dental Pulp and Periodontal Ligament of Deciduous Teeth. Tissue Eng Regen Med, 7:575-582, 2010.

Wang S, Gao X, Gong W, et al. : Odontogenic differentiation and dentin formation of dental pulp cells under nanobioactive glass induction. Acta Biomater, 10:2792-2803, 2014. crossref(new window)

Lei M, Li K, Li B, et al. : Mesenchymal stem cell characteristics of dental pulp and periodontal ligament stem cells after in vivo transplantation. Biomaterials, 35:6332-6343, 2014. crossref(new window)

Song JH, Park BW, Byun JH, et al. : Isolation and characterization of human dental tissue-derived stem cells in the impacted wisdom teeth: comparison of dental follicle, dental pulp, and root apical papilla- derived cells. J Korean Assoc Oral Maxillofac Surg, 36:186, 2010. crossref(new window)

Ruch JV : Odontoblast commitment and differentiation. Biochem Cell Biol, 76:923-938, 1998. crossref(new window)

Gronthos S, Mankani M, Brahim J, et al. : Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A, 97: 13625-13630, 2000. crossref(new window)

Miura M, Gronthos S, Zhao M, et al. : SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A, 100:5807-5812, 2003. crossref(new window)

Huang AH, Chen YK, Lin LM, et al. : Isolation and characterization of dental pulp stem cells from a supernumerary tooth. J Oral Pathol Med, 37:571- 574, 2008. crossref(new window)

Kim JB : Managing Complications Related to Multiple Supernumerary Teeth. J Korean Acad Pediatr Dent, 41:180-186, 2014. crossref(new window)

Huang WH, Tsai TP, Su HL : Mesiodens in the primary dentition stage: a radiographic study. ASDC J Dent Child, 59:186-189, 1992.

Burkhardt JK, Halama D, Frerich B, et al. : Realtime RT-PCR discriminating mRNA encoding osteocalcin from unspecific targets. Anal Bioanal Chem, 393:1351-1355, 2009. crossref(new window)

Turan S, Aydin C, Bereket A, et al. : Identification of a novel dentin matrix protein-1 (DMP-1) mutation and dental anomalies in a kindred with autosomal recessive hypophosphatemia. Bone, 46:402-409, 2010. crossref(new window)

D'souza R, Cavender A, Sunavala G, et al. : Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo. J Bone Miner Res, 12:2040-2049, 1997. crossref(new window)

De Vries IG, Quartier E, Boute P, et al. : Immunocytochemical localization of osteocalcin in developing rat teeth. J Dent Res, 66:784-790, 1987. crossref(new window)

Mundlos S, Schwahn B, Reichert T, et al. : Distribution of osteonectin mRNA and protein during human embryonic and fetal development. J Histochem Cytochem, 40:283-291, 1992. crossref(new window)

Reichert T, Storkel S, Becker K, et al. : The role of osteonectin in human tooth development: an immunohistological study. Calcif Tissue Int, 50:468- 472, 1992. crossref(new window)

Lian JB, Stein GS, Bortell R, et al. : Phenotype suppression: A postulated molecular mechanism for mediating the relationship of proliferation and differentiation by Fos/Jun interactions at AP-1 sites in steroid responsive promoter elements of tissue -specific genes. J Cell Biochem, 45:9-14, 1991. crossref(new window)

Livak KJ, Schmittgen TD : Analysis of relative gene expression data using real-time quantitative PCR and the 2-${\Delta}$${\Delta}$CT method. Methods, 25:402-408, 2001. crossref(new window)

Nanci A. Ten Cate's Oral Histology-Pageburst on VitalSource: Development, Structure, and Function: Elsevier Health Sciences; 2007.

Leso H : Odontoblast differentiation and tooth morphogenesis. J Dent Res, 79:1640, 2000. crossref(new window)

Butler WT, Ritchie H : The nature and functional significance of dentin extracellular matrix proteins. Int J Deve Biol, 39:169-179, 1995.

Gehron RP : The biochemistry of bone. Endocrinol Metab Clin North Am, 18:858-902, 1989.

Goseki M, Oida S, Nifuji A, et al. : Properties of alkaline phosphatase of the human dental pulp. J Dent Res, 69:909-912, 1990. crossref(new window)

Magnusson P, Larsson L, Magnusson M, et al. : Isoforms of bone alkaline phosphatase: characterization and origin in human trabecular and cortical bone. J Bone Miner Res, 14:1926-1933, 1999. crossref(new window)

Hanawa M, Takano Y, Wakita M : An autoradiographic study of calcium movement in the enamel organ of rat molar tooth germs. Arch Oral Biol, 35: 899-906, 1990. crossref(new window)

Papagerakis P, Berdal A, Mesbah M, et al. : Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth. Bone, 30:377-385, 2002. crossref(new window)

Pittenger MF, Mackay AM, Beck SC, et al. : Multilineage potential of adult human mesenchymal stem cells. Science, 284:143-147, 1999. crossref(new window)

Park JH, Song JS, Lee JH, et al. : Characteristics of Stem Cells Derived from the Periodontal Ligament of Supernumerary Teeth. Tissue Eng Regen Med, 8: 123-131, 2011.

Feng JQ, Luan X, Wallace J, et al. : Genomic organization, chromosomal mapping, and promoter analysis of the mouse dentin sialophosphoprotein (Dspp) gene, which codes for both dentin sialoprotein and dentin phosphoprotein. J Biol Chem, 273: 9457-9464, 1998. crossref(new window)

Kotobuki N, Hirose M, Funaoka H, et al. : Enhancement of in vitro osteoblastic potential after selective sorting of osteoblasts with high alkaline phosphatase activity from human osteoblast-like cells. Cell Transplant, 13:377-383, 2004. crossref(new window)