Advanced SearchSearch Tips
Effect of Extraction Methods on the Extraction Yield of Total Lipid and Arachidonic Acid from Single Cell Oil, Mortierella sp.
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Extraction Methods on the Extraction Yield of Total Lipid and Arachidonic Acid from Single Cell Oil, Mortierella sp.
Kim, Sun-Ki; Chung, Guk-Hoon; Han, Jeong-Jun; Cho, Sang Woo; Yoon, Suk Hoo;
  PDF(new window)
An oleaginous fungus was isolated from soil and identified as Mortierella sp. (M-12) for producing arachidonic acid (AA). Cell disruption methods, extraction methods, and particle sizes of freeze-dried biomass were tested to achieve maximum extraction of total lipids and AA. M-12 grown in glucose yeast media at for 7 days contained 35.5% total lipid, and 47% of the total lipid was AA. Lipid extraction yield from wet biomass was shown to be similar to that in a dry state. Maximum lipid extraction was achieved using a mixture of chloroform and methanol (2:1) as an extraction solvent. Different mechanical cell disruption methods did not affect lipid extraction yields. The smaller the particle size of the biomass, the better the lipid extraction yield was observed. Particle size of biomass was shown to more strongly affect lipid extraction than extraction time. The highest AA content was observed in the class of neutral lipids.
Mortierella sp.;single cell oil;lipid extraction;fatty acid composition;arachidonic acid;
 Cited by
미생물에 의한 단세포유지의 생산과 이용,김용노;윤석후;

한국식품과학회지, 2015. vol.47. 6, pp.687-697 crossref(new window)
모르티에렐라(Mortierella)속 유래 단세포유지로부터 추출한 지방질의 탈색,김선기;정국훈;한정준;조상우;윤석후;

한국식품과학회지, 2015. vol.47. 3, pp.405-408 crossref(new window)
Bleaching of Lipids Extracted from Single Cell Oil Produced by Mortierella sp., Korean Journal of Food Science and Technology, 2015, 47, 3, 405  crossref(new windwow)
Single Cell Oil-Recent Trends in Microbial Production and Utilization, Korean Journal of Food Science and Technology, 2015, 47, 6, 687  crossref(new windwow)
Fukaya T, Gondaira T, Kashiyae Y, Kotani S, Ishikura Y, Fujikawa S, Kiso Y, Sakakibara M. Arachidonic acid preserves hippocampal neuron membrane fluidity in senescent rats. Neurobiol. Aging 28: 1179-1186 (2007) crossref(new window)

Granstrom E. The arachidonic acid cascade. The prostaglandins, thromboxanes and leukotrienes. Inflammation 8: S15-25 (1984) crossref(new window)

Goodnight Jr SH, Harris WS, Connor WE, Illlngworth DR. Polyunsaturated Fatty acids, hyperlipidemia, and thrombosis. Arterioscl. Throm. Vas. 2: 87-113 (1982) crossref(new window)

Haag M. Essential fatty acids and the brain. Can. J. Psychiat. 48: 195-203 (2003) crossref(new window)

Mann NJ, Johnson LG, Warrick GE, Sinclair AJ. The arachidonic acid content of the Australian diet is lower than previously estimated. J. Nutr. 125: 2528-2535 (1995)

Park YS, Park HJ, Won SI. Association of Fatty Acid Intake and Dyslipidemia in Korean Adults: Korea National Health and Nutrition Survey, 1998-2007. J. East Asian Soc. Dietary Life 21: 789-807 (2011)

Makrides M, Neumann MA, Byard RW, Simmer K, Gibson RA. Fatty acid composition of brain, retina, and erythrocytes in breast- and formula-fed infants. Am. J. Clin. Nutr. 60: 189-194 (1994) crossref(new window)

Mann NJ, Johnson LG, Warrick GE, Sinclair AJ. The arachidonic acid content of the Australian diet is lower than previously estimated. J. Nutr. 125: 2528-2535 (1995)

Shinmen Y, Shimizu S, Akimoto K, Kawashima H, Yamada H. Production of arachidonic acid by Mortierella fungi. Appl. Microbiol. Biot. 31: 11-16 (1989)

Qi B, Fraser T, Mugford S, Dobson G, Sayanova O, Butler J, Napier JA, Stobart AK, Lazarus CM. Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nature Biotechnol. 22: 739-745 (2004) crossref(new window)

Eroshin VK, Dedyukhina EG, Chistyakova TI, Zhelifonova VP, Kurtzman CP, Bothast RJ. Arachidonic-acid production by species of Mortierella. World J. Microb. Biot. 12: 91-96 (1996) crossref(new window)

Ratledge C. Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie 86: 807-815 (2004) crossref(new window)

Zhu M, Zhou PP, Yu LJ. Extraction of lipids from Mortierella alpina and enrichment of arachidonic acid from the fungal lipids. Bioresource Technol. 84: 93-95 (2002) crossref(new window)

Manirakiza P, Covaci A, Schepens P. Comparative study on total lipid determination using Soxhlet, Roese-Gottlieb, Bligh & Dyer, and modified Bligh & Dyer extraction methods. J. Food Compos. Anal. 14: 93-100 (2001) crossref(new window)

Duthie AH, Patton S. Purification of phospholipids recovered from thin-layer chromatograms for infrared spectral analysis. J. Lipid Res. 6: 320-322 (1965)

Clarke PR, Hill CR. Physical and chemical aspects of ultrasonic disruption of cells. J. Acoust. Soc. Am. 47: 649-653 (1970) crossref(new window)

Dunn B, Wobbe CR. Preparation of protein extracts from yeast. Curr. Protoc. Mol. Biol. 13: 1-9 (2001)

Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, DH Byrne. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal. 19: 669-675 (2006) crossref(new window)

Rouser G, Kritchevsky G, Simon G, Nelson GJ. Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids. Lipids 2: 37-40 (1967) crossref(new window)

Freeman CP, West D. Complete separation of lipid classes on a single thin-layer plate. J. Lipid Res. 7: 324-327 (1966)

Lee SY, Jung MY, Yoon SH. Optimization of the refining process of camellia seed oil for edible purposes. Food Sci. Biotechnol. 23: 65-73 (2014) crossref(new window)

Eroshin VK, Satroutdinov AD, Dedyukhina EG, Chistyakova TI. Arachidonic acid production by Mortierella alpina with growthcoupled lipid synthesis. Process Biochem. 35: 1171-1175 (2000) crossref(new window)

Chen CS, Wu SH, Girdaukas G, Sih CJ. Quantitative analyses of biochemical kinetic resolution of enantiomers. 2. Enzyme-catalyzed esterifications in water-organic solvent biphasic systems. J. Am. Chem. Soc. 109: 2812-2817 (1987) crossref(new window)

Snyder JM, Friedrich JP, Christianson DD. Effect of moisture and particle size on the extractability of oils from seeds with supercritical $CO_2$. J. Am. Oil Chem. Soc. 61: 1851-1856 (1984) crossref(new window)