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Effects of Proteins on the Reactivity of Various Phenolic Compounds with the Folin-Ciocalteu Reagent
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 Title & Authors
Effects of Proteins on the Reactivity of Various Phenolic Compounds with the Folin-Ciocalteu Reagent
Park, Kyung A; Choi, Yoo-mi; Kang, Smee; Kim, Mi-Ri; Hong, Jungil;
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The Folin-Denis assay using the Folin-Ciocalteu (F-C) reagent has been commonly used for analyzing the total phenolic compound content in various food products. In the present study, the effects of proteins on the reactivity of the F-C reagent with different phenolic compounds were investigated. Bovine serum albumin (BSA) or skim milk proteins showed a concentration-dependent increase in color response in the Folin-Denis assay; these proteins decreased the color response of most phenolic compounds tested. The reactivity of phenolic compounds was significantly less pronounced in the presence of BSA and this interference was greater at higher concentrations of phenolic compounds. The reactivity of phenolic compounds with the F-C reagent was reduced significantly by their oxidation; the reaction of the oxidized products with the F-C reagent was more severely affected by BSA. The interfering effects in the Folin-Denis assay might be attributable to binding interactions of phenolic compounds with proteins.
phenolic compound;Folin-Denis assay;protein;bovine serum albumin;Folin-Ciocalteu reagent;
 Cited by
퉁퉁마디 지상부 분획의 산화방지 활성, 활성산소종 생성과 세포독성 조절작용,강스미;홍정일;

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