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Optimization of γ-Aminobutyric Acid (GABA) Production Using Immobilized Lactobacillus plantarum K154 in Submerged Culture of Ceriporia lacerata
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 Title & Authors
Optimization of γ-Aminobutyric Acid (GABA) Production Using Immobilized Lactobacillus plantarum K154 in Submerged Culture of Ceriporia lacerata
Lee, Eun-Ji; Lee, Sam-Pin;
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 Abstract
The production of GABA was optimized by co-cultivation of immobilized Lactobacillus plantarum K154 (ILK) with Ceriporia lacerata cultures. The mycelial culture of C. lacerata was performed in a defined medium containing 3% glucose, 3% soybean flour, and 0.15% in a submerged condition for 7 days at , resulting in the production of 29.7 g/L mycelia, 3.1 g/L exopolysaccharides, 2% (w/w) -glucan, 68.96 unit/mL protease, and 10.37 unit/mL -amylase. ILK in C. lacerata culture showed viable cell counts of for immobilized cells and for free cells after 1 day. GABA production in the free and immobilized cells was 9.96 mg/mL and 6.30 mg/mL, respectively, after 7 days. A recycling test of ILK in the co-fermentation was consequently performed five times at for 15 days, resulting in the highest production of GABA. GABA could also be efficiently overproduced by co-cultivation with the produced polysaccharides, -glucan, peptides, and probiotics.
 Keywords
Ca-alginate bead;immobilization;-aminobutyric acid;Lactobacillus plantarum;Ceriporia lacerata;
 Language
Korean
 Cited by
1.
막걸리 유래 미생물의 활용을 위한 연구 동향,이영석;설정만;정덕열;김수린;

한국미생물생명공학회지, 2016. vol.44. 3, pp.229-235 crossref(new window)
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