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Anti-adipogenic Effects of the Water Extracts of Defatted Green Tea Seed Cake
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 Title & Authors
Anti-adipogenic Effects of the Water Extracts of Defatted Green Tea Seed Cake
Wee, Ji-Hyang; Sung, Hea Mi; Jung, Kyung Ok; Kim, Suk Jung; Shin, Yu-Rim; Park, Ju-Hyun; Kim, Jong-Deog;
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The effect of the hot water extract of defatted green tea seed cake (GTSE) on lipid metabolism and the underlying mechanisms of lipolysis in mature 3T3-L1 adipocytes were investigated. In this study, we found that the naringenin content of GTSE was 5.5 mg/g; however, catechins were not detected. The intracellular lipid droplets were stained with Oil Red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 52%, and intracellular triglyceride (TG) level was reduced by 33% after treatment with GTSE at a concentration of . To determine the mechanism of reduction in TG content, we determined the level of fatty acid synthase (FAS), phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), and acetyl-coenzyme A carboxylase (ACC) in the cell model. Incubation of the 3T3-L1 adipocytes with GTSE stimulated AMPK and ACC phosphorylation in a dose-dependent manner, and decreased the expression of FAS.
Defatted green tea seed cake;naringenin;3T3-L1 adipocyte;lipolysis;
 Cited by
비파 부위별 추출물이 3T3-L1 세포와 돼지 지방전구세포의 분화에 미치는 효과,이환;이재준;

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