Advanced SearchSearch Tips
Simultaneous Determination of Caffeine, Theobromine and Theophylline in Tea Leaves by HPLC
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Simultaneous Determination of Caffeine, Theobromine and Theophylline in Tea Leaves by HPLC
Yun, Sang-Soon; Kim, Hyun; Jang, Su-Jin; Lim, Ho-Soo; Kim, Sheen Hee; Kim, Meehye;
  PDF(new window)
This study was aimed at investigating the natural occurrence of caffeine, theobromine, and theophylline in tea leaves. Sample clean-up was based on a simple solid phase extraction (SPE) treatment, and simultaneous analysis was performed by high performance liquid chromatography (HPLC). This method showed good results in terms of linearity, recovery, precision, limit of detection (LOD), and limit of quantitation (LOQ). The caffeine, theobromine, and theophylline contents of tea leaves (n
 Cited by
Kim HY, Lee YJ, Hong KH, Lee CW, Kim KS, Ha SC. Development of analysis method of caffeine and content survey in commercial foods by HPLC. Korean J. Food Sci. Technol. 31: 1471-1476 (1999)

Ashihara, H, Crozier A. Caffeine: A well known but little mentioned compound in plant science. Trends Plant Sci. 6: 407-413 (2001) crossref(new window)

Harland BF. Caffeine and Nutrition. Nutrition 16: 522-526 (2000) crossref(new window)

AOAC. Official Method of Analysis AOAC Intl. 19th ed. Method 979.11, 925.17. Association of Official Analytical Chemists, Arlington, VA, USA (2012)

Lau OW, Luk SF, Cheung YM. Simultaneous determination of ascorbic acid, caffeine and paracetamol in drug formulations by differential-pulse voltametry using a glassy carbon electrode. Analyst 114: 1047-1051 (1989) crossref(new window)

Newton JM. Spectrophotometric determination of caffeine in coffee products: Collaborative study. J. Assoc. Off. Ana. Chem. 62: 705-708 (1979)

Strahl NR, Lewis H, Fargen R. Comparison of gas chromatographic and spectrophotometric methods of determination for caffeine in teas. J. Agr. Food Chem. 25: 233-235 (1977) crossref(new window)

Blauch JL, Tarka jr SM. HPLC determination of caffeine and theobromine in coffee, tea and instant hot cocoa mixes. J. Food Sci. 48: 745-747 (1983) crossref(new window)

Perva-Uzunalic A, Skerget M, Knez Z, Weinreich B, Otto F, Gruner S. Extraction of active ingredients from green tea (Camellia sinesis): Extraction efficiency of major catechins and caffeine. Food Chem. 96: 597-605 (2006) crossref(new window)

de Aragao NM, Veloso MCC, Bispo MS, Ferreira SLC, de Andrade JB. Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversedphase high-performance liquid chromatography. Talanta 67: 1007-1013 (2005) crossref(new window)

Jun X. Caffeine extraction from green tea leaves assisted by high pressure processing. J. Food Eng. 94: 105-109 (2009) crossref(new window)

Hadad GM, Abdel Salam RA, Soliman RM, Mesbah MK. Rapid and simultaneous determination of antioxidant markers and caffeine in commercial teas and dietary supplements by HPLC-DAD. Talanta 101: 38-44 (2012) crossref(new window)

Bispo MS, Veloso MCC, Pinheiro HLC, de Oliveira RFS, Reis JON, de Andrade JB. Simultaneous determination of caffeine, theobromine, and theophylline by high-performance liquid chromatography. J. Chromatogr. Sci. 40: 45-48 (2002) crossref(new window)

FDA. Caffeine Intake by the U.S. Population. U.S. Food and Drug Administration. Kensington, CA, USA. pp 4 (2010)

Komes D, Horzic D, Belscak A, Kovacevic Ganic K, Baljak A. Determination of caffeine content in tea and mate tea by using different methods. Czech. J. Food Sci. 27: 213-216 (2009)

Zuo Y, Chen H, Deng Y. Simultaneous determination of catechins, caffeine and gallic acids in green, oolong, black and puerh teas using HPLC with a photodiode array detector. Talanta 57: 307-316 (2002) crossref(new window)

MFDS. Analysis method for food additives in food. Method 11.1. Ministry of Food and Drug Safety. Cheongju, Korea. (2014)

Chen QC, Mou SF, Hou XP, Ni ZM. Simultaneous determination of caffeine, theobromine and theophylline in foods and pharmaceutical preparations by using ion chromatography. Anal. Chim. Acta. 371: 287-296 (1998) crossref(new window)