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Cirsium japonicum Extracts Show Antioxidant Activity and PC12 Cell Protection against Oxidative Stress
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 Title & Authors
Cirsium japonicum Extracts Show Antioxidant Activity and PC12 Cell Protection against Oxidative Stress
Jang, Miran; Kim, Gun-Hee;
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The phenolic compounds, antioxidant activity and neuronal cell protective effect of Cirsium japonicum extract were evaluated in this study. High performance liquid chromatography mass analysis showed that C. japonicum was composed of chlorogenic acid, linarin, and pectolinarin. C. japonicum extract showed its antioxidant activity with half-maximal inhibitory concentrations of 567 and by DPPH and ABTS radical scavenging activity, respectively. The total antioxidant capacities of C. japonicum via DPPH, ABTS, and FRAP assays were 11.32, 100.15, and trolox equivalents, respectively. In addition, the neuroprotective effect of C. japonicum extract was investigated by measuring cell viability via MTT, LDH and DCF-DA assay using PC12 cells. C. japonicum extract showed neuronal cell protective effects in a dose-dependent manner. These results indicated that C. japonicum extract has potent antioxidant and neuronal protective effects. Therefore, C. japonicum can be regarded as an effective and safe functional food resource as natural antioxidants, and may decrease the risk of neurodegenerative disorders.
Cirsium japonicum;antioxidant activity;oxidative damage;PC12 cell;neuronal protective effect;
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음양곽 5종의 프레닐플라보노이드 함량에 따른 항산화 활성 및 산화적 스트레스에 대한 PC12 세포 보호효과,장미란;조천호;김건희;

원예과학기술지, 2017. vol.35. 6, pp.793-804 crossref(new window)
Lu YH, Su MY, Huang HY, Li L, Yuan CG. Protective effects of the citrus flavanones to PC12 cells against cytotoxicity induced by hydrogen peroxide. Neucosci. Lett. 484: 6-11 (2010) crossref(new window)

Esposito E, Rotilio D, Matteo VD, Giulio CD, Cacchio M, Algeri S. A review of specific dietary antioxidants and the effects on biochemical mechanisms related to neurodegenerative processes. Neurobiol. Aging 23: 719-735 (2002) crossref(new window)

Jeong CH, Kwak JH, Kim JH, Choi GN, Kim DO, Heo HJ. Neuronal cell protective and antioxidant effects of phenolics obtained from Zanthoxylum piperitum leaf using in vitro model system. Food Chem. 125: 417-422 (2011) crossref(new window)

Jang MR, Park HJ, Hong EY, Kim GH. Comparison of the antibacterial activity of domestic Cirsium japonicum collected from different regions. Korean J. Food Cook. Sci. 30: 278-283 (2014) crossref(new window)

Chon SU, Kim YM, Kim DK, He BG, Cho JY. Phytotoxic effect, DPPH radical scavenging activity and chlorogenic acid level of methanol extracts from aerial parts of several Korean salad plants. Korean J. Plant Resources 19: 405-410 (2006)

Ganzera M, Pocher P, Stuppner H. Differentiation of Cirsium japonicum and C. setosum by TLC and HPLC-MS. Phytochem. Anal. 16: 205-209 (2005) crossref(new window)

Ge H, Turhong M, Abudkrem M, Tang Y. Fingerprint analysis of Cirsium japonicum DC. using high performance liquid chromatography. J. Pharm. Anal. 3: 278-284 (2013) crossref(new window)

Jeong DM, Jung HA, Choi JS. Comparative antioxidant activity and HPLC profiles of some selected Korean thistles. Arch. Pharm. Res. 31: 28-33 (2008) crossref(new window)

Kim SJ, Kim GH. Identification for flavones in different parts of Cirsium japonicum. J. Food. Sci. Nutr. 8: 330-335 (2003) crossref(new window)

Greenlee H, Abascal K, Yarnell E, Ladas E. Clinical applications of Silybum marianum in oncology. Integr. Cancer. Ther. 6: 158-165 (2007) crossref(new window)

Jang MR, Hong EY, Cheong JH, Kim GH. Antioxidative components and activity of domestic Cirsium japonicum extract. J. Korean Soc. Food Sci. Nutr. 41: 739-744 (2012) crossref(new window)

Lee JH, Choi SI, Lee YS, Kim GH. Antioxidant and anti-inflammatory activities of ethanol extract from leaves of Cirsium japonicam. Food Sci. Biotech. 17: 38-45 (2008)

Yin Y, Heo SI, Wang MH. Antioxidant and anticancer activities of methanol and water extracts from leaves of Cirsium japonicam. J. Appl. Biol. Chem. 51: 160-164 (2008) crossref(new window)

Chon SU, Kim TS, Shin JS, Boo HO. Antioxidant activities of methanol extracts from root parts of Korean salad plants. Korean J. Plant Res. 21: 413-419 (2008)

Yin J, Heo SI, Wang MH. Antioxidant and antidiabetic activities of extracts from Cirsium japonicam roots. Nutr. Res. Pract. 2: 247-251 (2008) crossref(new window)

Kim EM, Won SI. Functional Composition and antioxidative activity from different organs of native Cirsium and Carduus genera. Korean J. Food Cook. Sci. 25: 406-414 (2009)

Mok JY, Kang HJ, Cho JK, Jeon IH, Kim HS, Park JM, Jeong SI, Shim JS, Jang SI. Antioxidative and anti-inflammatory effects of extracts from different organs of Cirsium japonicum var. ussuriense. Kor. J. Herbol. 26: 39-47 (2011)

Lee HK, Kim JS, Kim NY, Kim MJ, Park SU, Yu CY. Antioxidant, antimutagenicity and anticancer activities of extracts from Cirsium japonicum var. ussuriense KITAMURA. J. Med. Crop Sci. 11: 53-61 (2003)

Liu Y, Tia X, Gou L, Sun L, Ling X, Yin X. Luteolin attenuates diabetes-associated cognitive decline in rats. Brain Res. Bull. 94: 23-29 (2013) crossref(new window)

Han JY, Ahn SY, Kim CS, Yoo SK, Kim SK, Kim HC, Hong JT, Oh KW. Protection of apigenin against kainate-induced excitotoxicity by anti-oxidative effects. Biol. Pharm. Bull. 35: 1440-1446 (2012) crossref(new window)

Li G, Min BS, Zheng C, Lee J, OW SR, Ahn KS, Lee BK. Neuroprotective and free radical scavenging activities of phenolic compounds from Hovenia dulcis. Arch. Pharm. Res. 28: 804-809 (2005) crossref(new window)

Braca A, Politi M, Sanogo R, Sanou H, Morelli I, Pizza C, Tommasi ND. Chemical composition and antioxidant activity of phenolic compounds from wild and cultivated Sclerocarya birrea (Anacardiaceae) leaves. J. Agr. Food Chem. 51: 6689-6695 (2003) crossref(new window)

Wojdylo A, Oszmia ski J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 105: 940- 949 (2007) crossref(new window)

Heo HJ, Cho HY, Hong B, Kim HK, Kim EK, Kim BG, Shin DH. Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against A${\beta}$-induced oxidative stress in PC12 cells. Amyloid 8: 194-201 (2001) crossref(new window)

Prior RL, Xu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agr. Food Chem. 53: 4290-4302 (2005) crossref(new window)

Kwak JH, Choi GN, Park JH, Kim JH, Jeong HR, Jeong CH, Heo HJ. Antioxidant and neuronal cell protective effect of purple sweet potato extract. J. Agr. Life Sci. 44: 57-66 (2010)

Rice-Evans CA, Miller NJ, Paganga G. Paganga Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Bio. Med. 20: 933-956 (1996) crossref(new window)

Choi DY, Lee YJ, Hong JT, Lee HJ. Antioxidant properties of natural polyphenols and their therapeutic potentials for Alzheimer's disease. Brain Res. Bull. 10: 144-153 (2011)

Heo HJ, Choi SJ, Choi SG, Shin DH, Lee JM Lee CY. Effect of banana, orange, and apple on oxidative stress-induced neurotoxicity in PC12 cells. J. Food Sci. 73: H28-32 (2008) crossref(new window)

Lobner D. Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis? J. Neurosci. Meth. 96: 147-152 (2000) crossref(new window)

Zhao B. Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease. Neurochem. Res. 34: 630-638 (2009) crossref(new window)

Li Y, Shi W, Li Y, Zhou Y, Hua X, Song C, Ma H, Wang C, Li Y. Neuroprotective effects of chlorogenic acid against apoptosis of PC12 cells induced by methylmercury. Environ. Toxicol. Phar. 26: 13-21 (2008) crossref(new window)

Mikami Y, Yamazawa M. Chlorogenic acid, a polyphenol in coffee, protects neurons against glutamate neurotoxicity. Life Sci. 139: 69-74 (2015) crossref(new window)

Lou H, Fan P, Perez RG, Lou H. Neuroprotective effects of linarin through activation of the PI3K/Akt pathway in amyloid-${\beta}$- induced neuronal cell death. Bioorgan. Med. Chem. 19: 4021-4027 (2011) crossref(new window)