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Anti-Inflammatory Effect of Ethanol Extract from Onion (Allium cepa L.) Peel on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears
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 Title & Authors
Anti-Inflammatory Effect of Ethanol Extract from Onion (Allium cepa L.) Peel on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears
Ahn, Na-Kyung; Kang, Bo-Kyeong; Kim, Koth-Bong-Woo-Ri; Kim, Min-Ji; Bae, Nan-Young; Park, Ji-Hye; Park, Sun-Hee; Ahn, Dong-Hyun;
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Inflammation is a complex process involving a variety of immune cells, which defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. Onion peel contains several phenolic compounds, including quercetin at an amount 20 times greater in peel than edible flesh. Therefore, in this study, the anti-inflammatory effects of onion peel ethanol extract (OPEE) were investigated lipopolysaccharide-induced inflammatory response. In our results, NO production decreased in a dose-dependent manner. Secretion of IL-6, , and was suppressed by 44%, 53%, and 60% respectively, at . Moreover, OPEE also suppressed expression of COX-2, iNOS, , and MAPKs in a dose-dependent manner. Formation of mice ear edema was reduced at the highest dose tested compared to the control, and reduction of ear thickness was observed in the histological analysis as well. In the acute toxicity test, no morality was observed in mice administered 5,000 mg/kg body weight of OPEE over a 2-week observation period. These results suggest that OPEE may have significant effects on inflammatory factors and be a potential anti-inflammatory material.
anti-inflammatory effect;;MAPKs;pro-inflammatory cytokines;onion peel;
 Cited by
콘택트렌즈 다목적용액 적용을 위한 케르세틴과 트랜스-칼콘의 항균 활성,최지영;강종석;한종길;변종철;

한국안광학회지, 2016. vol.21. 4, pp.333-340 crossref(new window)
Antibacterial Activity of Quercetin and trans-Chalcone for Application in Contact Lens Multipurpose Solution, Journal of Korean Ophthalmic Optics Society, 2016, 21, 4, 333  crossref(new windwow)
Joung EM, Jung KH. 2014. Antioxidant activity of onion (Allium cepa L.) peel extracts obtained as onion byproducts. Korean J Food Sci Technol 46: 364-368. crossref(new window)

Lee HA, Han SJ, Hong SH, Kim OJ. 2014. Effects of onion peel water extract on the blood lipid profiles in mice fed a high-fat diet. Korean J Medicinal Crop Sci 22: 203-209. crossref(new window)

Pak JI, Kim YW, Seo TS, Jang A. 2012. Supplementation effect of onion peel extracts on small intestine of obese mice. J Life Sci 22: 1477-1486. crossref(new window)

Hatano T, Yasuhara T, Yoshihara R, Ikegami Y, Matsuda M, Yazaki K, Agata I, Nishibe S, Noro T, Yoshizaki M, Okuda T. 1991. Inhibitory effects of galloylated flavonoids on xanthine oxidase. Plant Med 57: 83-84. crossref(new window)

Bilyk A, Cooper PL, Sapers GM. 1984. Varietal differences in distribution of quercetin and kaempferol in onion tissue. J Agric Food Chem 32: 274-276. crossref(new window)

Middleton E. 1998. Effect of plant flavonoids on immune and inflammatory cell function. Adv Exp Med Biol 439: 175-182. crossref(new window)

Byun MW. 2012. Anti-inflammatory activity of austroinulin from Stevia rebaudiana in LPS-induced RAW264.7 cells. J Korean Soc Food Sci Nutr 41: 456-461. crossref(new window)

Jeong DH, Kim KB, Kim MJ, Kang BK, Ahn DH. 2013. Anti-inflammatory activity of ethanolic extract of Sargassum micracanthum. J Microbiol Biotechnol 23: 1691-1698. crossref(new window)

Ko YJ, Song SM, Hyun WC, Yang SK, Song CK, Lee DS, Yoon WJ. 2014. Anti-inflammatory effect of Castanopsis cuspidata extracts in murine macrophage RAW 264.7 cells. Korean J Plant Res 27: 439-446. crossref(new window)

Kim MJ, Bae GS, Choi SB, Jo IJ, Kim DG, Shin JY, Lee SK, Kim MJ, Park SJ, Song HJ. 2014. The anti-inflammatory effect of Taraxacum coreanum on lipopolysaccharide induced inflammatory response on RAW 264.7 cells. Kor J Herbology 29: 21-26.

Jeong DH, Kim KB, Kim MJ, Kang BK, Ahn DH. 2014. Anti-inflammatory activity of methanol extract and n-hexane fraction mojabanchromanol b from Myagropsis myagroides. Life Sci 114: 12-19. crossref(new window)

Jeon SM, Choi MH, Lee YJ, Shin HJ. 2013. Anti-wrinkle effect of facial mask pack containing onion (Allium cepa) skin extracts. Korean Soc Biotechnol Bioeng J 28: 387-393.

Lee ST, Jeong YR, Ha MH, Kim SH, Byun MW, Jo SK. 2000. Induction of nitric oxide and TNF-${\alpha}$ by herbal plant extracts in mouse macrophages. J Korean Soc Food Sci Nutr 29: 342-348.

Sheeba MS, Asha VV. 2009. Cardiospermum halicacabum ethanol extract inhibits LPS induced COX-2, TNF-${\alpha}$ and iNOS expression, which is mediated by NF-${\kappa}B$ regulation, in RAW264.7 cells. J Ethnopharmacol 124: 39-44. crossref(new window)

Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. crossref(new window)

Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354. crossref(new window)

Kim DW, Chi YS, Son KH, Chang HW, Kim JS, Kang SS, Kim HP. 2002. Effects of sophoraflavanone G, a prenylated flavonoid from Sophora flavescens, on cyclooxygenase-2 and in vivo inflammatory response. Arch Pharm Res 25: 329-335. crossref(new window)

Saraiva RA, Araruna MK, Oliveira RC, Menezes KD, Leite GO, Kerntopf MR, Costa JG, Rocha JB, Tome AR, Campos AR, Menezes IR. 2011. Topical anti-inflammatory effect of Caryocar coriaceum Wittm. (Caryocaraceae) fruit pulp fixed oil on mice ear edema induced by different irritant agents. J Ethnopharmacol 136: 504-510. crossref(new window)

OECD. 2002. OECD guidelines for the testing of chemicals, section 4: health effects. Test No. 420: acute oral toxicityfixed dose procedure. download/9742001e.pdf?expires=1442476991&id=id&accname= guest&checksum=FBBD152B70A8C56DA16EBF8D BA7B164B (accessed Jun 2015).

Lee E, Kim H, Yu J, Cho Y, Kim D, Shin Y, Cho Y, Kwon O, An B. 2014. Anti-inflammatory effect of Polygonum multiflorum extraction in activated RAW 264.7 cells with lipopolysaccharide. Korean J Food Preserv 21: 740-746. crossref(new window)

Kim CH, Lee MA, Kim TW, Jang JY, Kim HJ. 2012. Antiinflammatory effect of Allium hookeri root methanol extract in LPS-induced RAW264.7 cells. J Korean Soc Food Sci Nutr 41: 1645-1648. crossref(new window)

Ko WM, Lee DS, Jang M, Kim KS, Lee HS, Baek HY, Oh H, Kim YC. 2013. A comparison of anti-inflammatory effects of Sophorae Flos EtOH extracts at the different flowering stages on LPS-induced inflammation in RAW264.7 cells. Kor J Pharmacogn 44: 291-297.

Stankiewicz W, Dabrowski MP, Chcialowski A, Plusa T. 2002. Cellular and cytokine immunoregulation in patients with chronic obstructive pulmonary disease and bronchial asthma. Mediators Inflammation 11: 307-312. crossref(new window)

Beutler B, Cerami A. 1989. The biology of cachectin/TNF-${\alpha}$primary mediator of the host response. Annu Rev Immunol 7: 625-655. crossref(new window)

Liu M, Li J, Kong F, Lin J, Gao Y. 1998. Induction of immunomodulating cytokines by a new polysaccharide-peptide complex from culture mycelia of Lentinus edodes. Immunopharmacology 40: 187-198. crossref(new window)

Dinarello CA, Endres S, Meydani SN, Meydani M, Hellerstein MK. 1990. Interleukin-1, anorexia, and dietary fatty acids. Ann N Y Acad Sci 587: 332-338. crossref(new window)

Kang BK, Kim KBWR, Ahn NK, Choi YU, Kim M, Bark SW, Park WM, Kim BR, Park JH, Bae NY, Ahn DH. 2015. Anti-inflammatory effect of onion (Allium cepa) peel hot water extract in vitro and in vivo. Korean Soc Biotechnol Bioeng J 30: 148-154.

Ito T, Ikeda U. 2003. Inflammatory cytokines and cardiovascular disease. Curr Drug Targets Inflamm Allergy 2: 257-265. crossref(new window)

Bishop-Bailey D, Calatayud S, Warner TD, Hla T, Mitchell JA. 2002. Prostaglandins and the regulation of tumor growth. J Environ Pathol Toxicol Oncol 21: 93-101.

Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH. 2012. Anti-inflammatory effects of extracts from Ligustrum ovalifolium H. leaves on RAW264.7 macrophages. J Korean Soc Food Sci Nutr 41: 1205-1210. crossref(new window)

Ghosh S, Hayden MS. 2008. New regulators of NF-${\kappa}B$ in inflammation. Nat Rev Immunol 8: 837-848. crossref(new window)

Giuliani C, Napolitano G, Bucci I, Montani V, Monaco F. 2001. NF-${\kappa}B$ transcription factor: role in the pathogenesis of inflammatory, autoimmune, and neoplastic diseases and therapy implications. Clin Ter 152: 249-253.

Hwang SI, Ju HJ, Kim HD, Kwak TW, Yoo BH, Kim SG, Lee CW, Hwang TH, An WG. 2011. Anti-inflammatory effect of fermented red ginseng via regulation of NF-${\kappa}B$ signal transduction. Korean J Oriental Physiology & Pathology 25: 521-527.

Johnson GL, Lapadat R. 2002. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298: 1911-1912. crossref(new window)

Kim JE, Kim SB, Kang OH, Shin IS, Kang SH, Lee SH, Kwon DY. 2013. Inhibitory effect of water extract from Dojuksan on LPS-induced proinflammatory cytokines production in RAW 264.7 cells. Kor J Herbology 28: 53-60.

Kim BH, Lee YT, Kang KH. 2010. Codonopsis lanceolata inhibits inflammation through regulation of MAPK in LPSstimulated RAW264.7 cells. Korean J Oriental Physiology & Pathology 24: 80-84.

Ju MS, Jeong HU, Kim HG, Park GH, Youn YS, Kim YO, Kim SY, Oh MS. 2010. Anti-nociceptive and anti-inflammatory effects of Geranii Herba. Kor J Herbology 25: 97-101.