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Comparison Study of Immunomodulatory Activity of Polysaccharide and Ethanol Extracted from Sargassum fulvellum
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 Title & Authors
Comparison Study of Immunomodulatory Activity of Polysaccharide and Ethanol Extracted from Sargassum fulvellum
Byun, Eui-Hong;
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The immune system plays an important role in maintaining and protecting human health. In the present study, comparison of immuno-modulatory activities between polysaccharides (SFP) and ethanol (SFE) extracts separated from Sargassum fulvellum in macrophages and murine splenocytes were investigated. Immuno-modulatory activities of macrophages were estimated based on cell proliferation, nitric oxide (NO), inducible NO synthase (iNOS), and cytokine production in RAW 264.7 macrophage cells, and lipopolysaccharide was used as a positive control. SFP and SFE treatment did not affect cytotoxicity in RAW 264.7 macrophage cells, and SFP treatment significantly increased NO and cytokine production (, IL-6, and ), whereas SFE did not contribute to the increase in NO and cytokine production. In the case of splenocytes, SFP treatment increased splenocyte proliferation and also highly increased production of Th-1 type cytokines (IL-2 and ) than those of SFE. Through this study, we confirmed that immuno-modulatory activities of Sargassum fulvellum may be due to polysaccharide extracts and this can be a potential nutraceutical.
Sargassum fulvellum;polysaccharide;ethanol extracts;immuno-modulatory activity;cytokine;
 Cited by
Hoebe K, Janssen E, Beutler B. 2004. The interface between innate and adaptive immunity. Nat Immunol 5: 971-974.

Kim HS, Kang JS. 2008. Preparation and characteristics of bread by medicinal herb composites with immunostimulating activity. J Korean Soc Food Sci Nutr 37: 109-116. crossref(new window)

Kim SW. 1998. Studies on anti-microbial and anti-cancer functions of polysaccharide extracted from Ganoderma lucidum. J Korean Soc Food Sci Nutr 27: 1183-1188.

Zhu H, Zhang Y, Zhang J, Chen D. 2008. Isolation and characterization of an anti-complementary protein-bound polysaccharide from the stem barks of Eucommia ulmoides. Int Immunopharmacol 8: 1220-1230.

Wang HX, Liu WK, Ng TB, Ooi VE, Chang ST. 1995. Immunomodulatory and antitumor activities of a polysaccharidepeptide complex from a mycelial culture of Tricholoma sp., a local edible mushroom. Life Sci 57: 269-281. crossref(new window)

Yadomae T. 2000. Structure and biological activities of fungal ${\beta}$-1,3 glucans. Yakugaku Zasshi 120: 413-431.

Hikino H, Kobayashi M, Suzuki Y, Konno C. 1989. Mechanism of hypoglycemic activity of aconitan A, a glycan from Aconitum carmichaeli roots. J Ethnopharmacol 25: 295-304. crossref(new window)

Bae SJ. 2004. Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33: 480-486. crossref(new window)

Tibbot BK, Skadsen RW. 1996. Molecular cloning and characterization of a gibberellin-inducible, putative ${\alpha}$-glucosidase gene from barley. Plant Mol Biol 30: 229-241. crossref(new window)

Kong CS, Um YR, Lee JI, Kim YA, Lee JS, Seo Y. 2008. Inhibition effects of extracts and its solvent fractions isolated from Limonium tetragonum on growth of human cancer cells. Korean J Biotechnol Bioeng 23: 177-182.

Heo SJ, Park EJ, Lee KW, Jeon YJ. 2005. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresour Technol 96: 1613-1623. crossref(new window)

Heo SJ, Jeon YJ. 2005. Antioxidant effect and protecting effect against cell damage by enzymatic hydrolysates form marine algae. Food Industry and Nutrition 10(1): 31-41.

Jeong DH, Kim KBWR, Kang BK, Jung SA, Kim HJ, Jeong HY, Park SW, Ahn DH. 2012. Anti-inflammatory activity of the water extract of Sargassum fulvellum. KSBB J 27: 325-329. crossref(new window)

Jeong DH, Kim KBWR, Kim MJ, Kang BK, Bark SW, Pak WM, Kim BR, Ahn NK, Choi YU, Ahn DH. 2014. Anti-inflammatory effect of ethanol extract from Sargassum fulvellum on lipopolysaccharide induced inflammatory responses in RAW 264.7 cells and mice ears. J Korean Soc Food Sci Nutr 43: 1158-1165. crossref(new window)

Sunderkotter C, Steinbrink K, Goebeler M, Bhardwaj R, Sorg C. 1994. Macrophages and angiogenesis. J Leukoc Biol 55: 410-422.

Morel F, Doussiere J, Vignais PV. 1991. The superoxide-generating oxidase of phagocytic cells. Physiological, molecular and pathological aspects. Eur J Biochem 201: 523-546. crossref(new window)

Ramesh HP, Yamaki K, Tsushida T. 2002. Effect of fenugreek (Trigonella foenum-graecum L.) galactomannan fractions on phagocytosis in rat macrophages and on proliferation and IgM secretion in HB4C5 cells. Carbohyd Polym 50: 79-83. crossref(new window)

Wink DA, Hines HB, Cheng RY, Switzer CH, Flores-Santana W, Vitek MP, Ridnour LA, Colton CA. 2011. Nitric oxide and redox mechanisms in the immune response. J Leukoc Biol 89: 873-891. crossref(new window)

Alderton WK, Cooper CE, Knowles RG. 2001. Nitric oxide synthases: structure, function and inhibition. Biochem J 357: 593-615. crossref(new window)

Tintinalli JE, Kelen GD, Stapczynski JS. 2003. Emergency medicine: A comprehensive study guide. 6th ed. McGraw Hill, New York, NY, USA. p 231-242.

Kim JY, Jung KS, Jeong HG. 2004. Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Lett 569: 321-326. crossref(new window)

Wang H, Actor JK, Indrigo J, Olsen M, Dasgupta A. 2003. Asian and Siberian ginseng as a potential modulator of immune function: an in vitro cytokine study using mouse macrophage. Clin Chim Acta 327: 123-128. crossref(new window)

Ryu HS. 2008. Effects of Job's Tears (Yul-Moo) extracts on mouse splenocyte and macrophage cell activation. Korean J Food & Nutr 21: 1-6.

Cha JH, Kim YS, Lee EM. 2010. Effects of Prunellae Spica water extract on immune response in macrophage cells. J Oriental Obstet Gynecol 23: 91-100.

Ryu HS, Kim J, Kim HS. 2006. Enhancing effect of Sorghum bicolor L. Moench (sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Korean J Food & Nutr 19: 176-182.

Cyster JG. 1999. Chemokines and cell migration in secondary lymphoid organs. Science 286: 2098-2102. crossref(new window)

Zalys R, Zagon IS, Bonneau RH, Lang CM, McLaughlin PJ. 2000. In vivo effects of chronic treatment with [$MET^5$]-enkephalin on hematological values and natural killer cell activity in athymic mice. Life Sci 66: 829-834. crossref(new window)

Abbas AK, Lichtman AH. 2003. Cell and tissues of the immune system. In Cellular and Molecular Immunology. 5th ed. WB Saunders Co., Sydney, Australia. p 264-269.

Wang JE, Jorgensen PF, Ellingsen EA, Almiof M, Thiemermann C, Foster SJ, Aasen AO, Solberg R. 2001. Peptidoglycan primes for LPS-induced release of proinflammatory cytokines in whole human blood. Shock 16: 178-182. crossref(new window)

Medzhitov R. 2001. Toll-like receptors and innate immunity. Nat Rev Immunol 1: 135-145. crossref(new window)

Schaeffer DJ, Krylov VS. 2000. Anti-HIV activity of extracts and compounds from algae and cyanobacteria. Ecotoxicol Environ Safe 45: 208-227. crossref(new window)