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Effect of Supercritical Carbon Dioxide on Physicochemical Properties and Microbial Reduction of Freeze-Dried Bovine Liver
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Effect of Supercritical Carbon Dioxide on Physicochemical Properties and Microbial Reduction of Freeze-Dried Bovine Liver
Kim, Hye-Min; Woo, Sung-Woon; Kim, Ah-Na; Heo, Ho-Jin; Chun, Ji-Yeon; Choi, Sung-Gil;
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Supercritical carbon dioxide () treatment has been becoming an important method for substituting the use of organic solvents for samples extraction prior to analysis due to its low toxicity, ease of handling, low cost of disposal etc. Freeze-dried bovine liver was treated with under different pressures (200, 300, and 450 bar) in order to investigate effects on physicochemical properties and reduction of microbial load. The yield of lipid extraction from bovine liver by treatment increased with increasing pressure, with values of 84, 86, and 90% in response to 200, 300, and 450 bar, respectively. Results of high performance liquid chromatography analysis showed that vitamin A and coenzyme (), which is soluble in lipid, were almost removed from bovine liver by treatment. Saturated fatty acids ratio of bovine liver decreased with increasing pressure, whereas polyunsaturated fatty acids increased with increasing pressure. Total content of amino acids in bovine liver treated by was less than that of the control sample without treatment. The number of aerobic bacteria in bovine liver, which was stored at for 5 days and freeze-dried, decreased from 6.2 to 4.2 log CFU/g by treatment at 100 bar for 3 h. Interestingly, coliform bacteria were not found in the bovine liver sample by at 100 bar for 3 h under all storage conditions. This indicates that treatment can effectively reduce coliform bacteria in the food matrix even at low moisture. In conclusion, freeze-dried bovine liver by proper treatment may be used as a potential high protein source, with increasing microbial safety and stability of lipid oxidation.
supercritical carbon dioxide;physicochemical properties;microbial;reduction;bovine liver powder;
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