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Convenient Method for Selective Isolation of Immuno-Stimulating Polysaccharides from Persimmon Leaves
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 Title & Authors
Convenient Method for Selective Isolation of Immuno-Stimulating Polysaccharides from Persimmon Leaves
Lee, Sue Jung; Hong, Hee-Do; Shin, Kwang-Soon;
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The biological activity of polysaccharide is greatly influenced by polysaccharide structure and molecular distribution. Here, we developed a rapid and convenient isolation method for fractionating polysaccharides with different characteristics and optimized it using a polysaccharide mixture from Korean persimmon leaves. A crude polysaccharide mixture, persimmon leaves-enzyme (PLE) fraction, was isolated from persimmon leaves digested with pectinase and ethanol precipitation. The PLE fraction was further fractionated with a serially diluted ethanol solution (ethanol : deionized water=4:1, 2:1, 1.5:1, 1:1, and 0.5:1) to produce 10 subfractions (five precipitate fractions labeled from PLE-4 to PLE-0.5 and five supernatant fractions labeled from PLE-4S to PLE-0.5S). HPLC analysis indicated that PLE-4 and -2 consisted of diverse polysaccharides, whereas PLE-1.5, -1, and -0.5 contained high molecular weight (MW) polysaccharides. The fractions from PLE-4 to PLE-1 were mostly composed of 13 different characteristic sugars in rhamnogalacturonan (RG) I and II, and the sugars contained an arabino--3,6-galactan moiety. However, PLE-0.5 did not contain RG-II or -arabino-3,6-galactan. Treatment of macrophages with fractions PLE-1.5S and PLE-1S led to a increase in interleukin (IL)-6 production, whereas treatment with PLE-4S and PLE-2S fractions composed of low MW polysaccharides resulted in reduced levels of IL-6. These results indicate that this isolation method may be useful for the rapid and convenient fractionation of bioactive RGs from polysaccharide mixtures with various properties.
persimmon leaves;isolation method;immuno-stimulating activity;polysaccharide;rhamnogalacturonan;
 Cited by
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