Advanced SearchSearch Tips
Anti-Inflammatory Effect of Chondrus nipponicus Yendo Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Anti-Inflammatory Effect of Chondrus nipponicus Yendo Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells
Kim, Min-Ji; Bae, Nan-Yong; Kim, Koth-Bong-Woo-Ri; Park, Ji-Hye; Park, Sun-Hee; Jang, Mi-Ran; Ahn, Dong-Hyun;
  PDF(new window)
The anti-inflammatory activity of ethanol extract from Chondrus nipponicus Yendo (CNYEE) was investigated by measuring production of a lipopolysaccharide-induced inflammatory response mediator. CNYEE had no cytotoxic effects on proliferation of macrophages compared to the control. CNYEE significantly inhibited (over 50%) NO production at , with inhibitory effects on expression levels of cytokines such as interleukin (IL)-6, tumor necrosis factor- (TNF-), and IL-. In particular, IL-6 inhibitory activity of CNYEE was higher than 70% at . CNYEE also reduced protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor (NF)- in a dose-dependent manner. CNYEE also significantly reduced phosphorylation of p38, extracellular signal-regulated kinase, and c-Jun N-terminal kinase. Therefore, these results suggest that CNYEE may have anti-inflammatory effects by modulating the NF- and mitogen-activated protein kinases signaling pathways and may be used as an anti-inflammatory therapeutic material.
Chondrus nipponicus Yendo;anti-inflammatory;cytokine;MAPKs;
 Cited by
Willoughby DA. 1975. Heberden Oration, 1974. Human arthritis applied to animal models. Towards a better therapy. Ann Rheum Dis 34: 471-478. crossref(new window)

Miyake K. 2004. Innate recognition of lipopolysaccharide by Toll-like receptor 4-MD-2. Trends Microbiol 12: 186-192. crossref(new window)

Kim YW, Zhao RJ, Park SJ, Lee JR, Cho IJ, Yang CH, Kim SG, Kim SC. 2008. Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-${\kappa}B$-dependent iNOS and proinflammatory cytokines production. Br J Pharmacol 154: 165-173. crossref(new window)

Dhawan P, Richmond A. 2002. A novel NF-${\kappa}B$-inducing kinase-MAPK signaling pathway up-regulates NF-${\kappa}B$ activity in melanoma cells. J Biol Chem 277: 7920-7928. crossref(new window)

Hofseth LJ, Ying L. 2006. Identifying and defusing weapons of mass inflammation in carcinogenesis. Biochim Biophys Acta 1765: 74-84.

Jeong DH, Kim KBWR, Kang BK, Jung SA, Kim HJ, Jeong HY, Bark SW, Ahn DH. 2012. Anti-inflammatory activity of the Undaria pinnatifida water extract. J Appl Biol Chem 55: 221-225. crossref(new window)

Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Kim BR, Ahn NK, Choi YU, Ahn DH. 2014. Anti-inflammatory activity of an ethanol extract of Laminaria japonica root on lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Korean J Food Sci Technol 46: 729-733. crossref(new window)

Lee JH, Kim ND, Choi JS, Kim YJ, Heo MY, Lim SY, Park KY. 1998. Inhibitory effects of the methanolic extract of an edible brown alga, Ecklonia stolonifera and its component, phloroglucinol on aflatoxin $B_1$ mutagenicity in vitro (Ames test) and on benzo(a)pyrene or N-methyl N-nitrosourea clastogenicity in vivo (mouse micronucleus test). Nat Prod Sci 4: 105-114.

Kim HS, Kim GJ. 1998. Effects of the feeding Hizikia fusiforme (Harvey) Okamura on lipid composition of serum in dietary hyperlipidemic rats. J Korean Soc Food Sci Nutr 27: 718-723.

Nagayama K, Iwamura Y, Shibata T, Hirayama I, Nakamura T. 2002. Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J Antimicrob Chemother 50: 889-893. crossref(new window)

Bae SJ. 2004. Studies on the antioxidative and antimicrobial effects of Chondria crassicaulis. J Life Sci 14: 411-416. crossref(new window)

Shin JH, Choi DJ, Lim HC, Seo JK, Lee SJ, Choi SY, Sung NJ. 2006. Nutrients and antioxidant activity of red seaweeds. J Life Sci 16: 400-408. crossref(new window)

Bae NY, Kim MJ, Kim KBWR, Ahn NK, Choi YU, Park JH, Park SH, Ahn DH. 2015. Anti-inflammatory effect of ethanol extract from Grateloupia elliptica Holmes on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells and mice ears. J Korean Soc Food Sci Nutr 44: 1128-1136. crossref(new window)

Park YM, Won JH, Yun KJ, Ryu JH, Han YN, Choi SK, Lee KT. 2006. Preventive effect of Ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression of nuclear factor-${\kappa}B$ in RAW 264.7 cells. Biol Pharm Bull 29: 985-990. crossref(new window)

Lee ST, Jeong YR, Ha MH, Kim SH, Byun MW, Jo SK. 2000. Induction of nitric oxide and TNF-${\alpha}$ by herbal plant extracts in mouse macrophages. J Korean Soc Food Sci Nutr 29: 342-348.

Sheeba MS, Asha VV. 2009. Cardiospermum halicacabum ethanol extract inhibits LPS induced COX-2, TNF-${\alpha}$ and iNOS expression, which is mediated by NF-${\kappa}B$ regulation, in RAW264.7 cells. J Ethnopharmacol 124: 39-44. crossref(new window)

Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. crossref(new window)

Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354. crossref(new window)

Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Ahn NK, Choi YU, Bae NY, Park JH, Ahn DH. 2015. Anti-inflammatory effect of Sargassum coreanum ethanolic extract through suppression of NF-${\kappa}B$ pathway in LPS induced RAW264.7 cells in mice. Microbiol Biotechnol Lett 43: 112-119.

Jeong H, Sung M, Kim Y, Ham H, Choi Y, Lee J. 2012. Anti-inflammatory activity of Salvia plebeia R. Br. leaf through heme oxygenase-1 induction in LPS-stimulated RAW 264.7 macrophages. J Korean Soc Food Sci Nutr 41: 888-894. crossref(new window)

Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH. 2012. Anti-inflammatory effects of extracts from Ligustrum ovalifolium H. leaves on RAW264.7 macrophages. J Korean Soc Food Sci Nutr 41: 1205-1210. crossref(new window)

Kim MJ, Bae NY, Kim KBWR, Park JH, Park SH, Cho YJ, Ahn DH. 2015. Anti-inflammatory effect of Zostera marina ethanolic extract on LPS-induced RAW264.7 cells and mouse model. Korean Soc Biotechnol Bioeng J 30: 182-190.

Majdalawieh A, Ro HS. 2010. Regulation of $I{\kappa}B{\alpha}$ function and NF-${\kappa}B$ signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators Inflamm 2010: 823821.

Hyun TK, Ko YJ, Kim EH, Chung IM, Kim JS. 2015. Anti-inflammatory activity and phenolic composition of Dendropanax morbifera leaf extracts. Ind Crops Prod 74: 263-270. crossref(new window)

Nathan C, Xie QW. 1994. Nitric oxide synthases: roles, tolls, and controls. Cell 78: 915-918. crossref(new window)

Bhattacharyya A, Pathak S, Datta S, Chattopadhyay S, Basu J, Kundu M. 2002. Mitogen-activated protein kinases and nuclear fator-${\kappa}B$ regulate Helicobacter pylori-mediated interleukin-8 release from macrophages. Biochem J 368: 121-129. crossref(new window)

Chen X, Miao J, Wang H, Zhao F, Hu J, Gao P, Wang Y, Zhang L, Yan M. 2015. The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-${\kappa}B$ pathway. J Ethnopharmacol 170: 72-80. crossref(new window)

Lebovic DI, Bentzien F, Chao VA, Garrett EN, Meng YG, Taylor RN. 2000. Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-$1{\beta}$. Mol Hum Reprod 6: 269-275. crossref(new window)

Jeong DH, Kim KBWR, Kim MJ, Kang BK, Bark SW, Pak WM, Kim BR, Ahn NK, Choi YU, Ahn DH. 2014. Anti-inflammatory effect of ethanol extract from Sargassum fulvellumon lipopolysaccharide induced inflammatory responses in RAW 264.7 cells and mice ears. J Korean Soc Food Sci Nutr 43: 1158-1165. crossref(new window)

Kim DH, Hwang EY, Son JH. 2013. Anti-inflammatory activity of Carthamus tintorious seed extract in Raw 264.7 cells. J Life Sci 23: 55-62. crossref(new window)

Miyasaka N, Hirata Y. 1997. Nitric oxide and inflammatory arthritides. Life Sci 61: 2073-2081. crossref(new window)

Simmons ML, Murphy S. 1992. Induction of nitric oxide from glial cells. J Neurochem 59: 897-905. crossref(new window)

Martel-Pelletier J, Pelletier JP, Fahmi H. 2003. Cyclooxygenase-2 and prostaglandins in articular tissues. Semin Arthritis Rheum 33: 155-167. crossref(new window)

Needleman P, Isakson PC. 1997. The discovery and function of COX-2. J Rheumatol 49: 6-8.

Seybold VS, Jia YP, Abrahams LG. 2003. Cyclo-oxygenase-2 contributes to central sensitization in rats with peripheral inflammation. Pain 105: 47-55. crossref(new window)

Xie QW, Whisnant R, Nathan C. 1993. Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon ${\gamma}$ and bacterial lipopolysaccharide. J Exp Med 177: 1779-1784. crossref(new window)

Robinson MJ, Cobb MH. 1997. Mitogen-activated protein kinase pathways. Curr Opin Cell Biol 9: 180-186. crossref(new window)

Dong C, Davis RJ, Flavell RA. 2002. MAP kinases in the immune response. Annu Rev Immunol 20: 55-72. crossref(new window)

Kim HG, Shrestha B, Lim SY, Yoon DH, Chang WC, Shin DJ, Han SK, Park SM, Park JH, Park HI, Sung JM, Jang Y, Chung N, Hwang KC, Kim TW. 2006. Cordycepin inhibits lipopolysaccharide-induced inflammation by the suppression of NF-${\kappa}B$ through Akt and p38 inhibition in RAW 264.7 macrophage cells. Eur J Pharmacol 545: 192-199. crossref(new window)

Park JS, Lee EJ, Lee JC, Kim WK, Kim HS. 2007. Anti-inflammatory effects of short chain fatty acids in IFN-${\gamma}$-stimulated RAW 264.7 murine macrophage cells: involvement of NF-${\kappa}B$ and ERK signaling pathways. Int Immunopharmacol 7: 70-77. crossref(new window)