Advanced SearchSearch Tips
Antioxidant Effects of Eriodictyol on Hydrogen Peroxide-Induced Oxidative Stress in HepG2 Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Antioxidant Effects of Eriodictyol on Hydrogen Peroxide-Induced Oxidative Stress in HepG2 Cells
Joo, Tae-Woo; Hong, Sung-Hyun; Park, Sun-Young; Kim, Gur-Yoo; Jhoo, Jin-Woo;
  PDF(new window)
This study was conducted to investigate the antioxidant and hepatoprotective effects of eriodictyol compound against hydrogen peroxide-induced oxidative stress in HepG2 cells by measuring expression levels of antioxidant enzymes, liver function index enzyme activities, and inhibitory effects against reactive oxygen species (ROS) production. HepG2 cell viability was assessed using 3-(4,5-dimethyl thiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. In the concentration range of , eriodictyol displayed over 98% cell viability in HepG2 cells. The effects of increased gene expression on hydrogen peroxide-induced oxidative stress were analyzed by monitoring antioxidant enzyme (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx) gene expression levels using real-time PCR. Eriodictyol compound significantly increased gene expression levels of SOD, CAT, and GPx in a dose-dependent manner (). Hepatoprotective effects against hydrogen peroxide-induced oxidative stress were analyzed by monitoring glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) activities in HepG2 cell culture medium using a biochemistry analyzer. Eriodictyol compound significantly reduced GOT, LDH, and GGT activities in a dose-dependent manner in HepG2 cells. ROS level in HepG2 cells was analyzed by 2',7'-dichlorofluorescein fluorescence diacetate assay, and eriodictyol compound effectively reduced the intracellular ROS level in HepG2 cells. The results reveal that eriodictyol compound can be useful for development of effective antioxidant and hepatoprotective agents.
eriodictyol;antioxidant enzyme expression;hepatoprotective;flavonoid;antioxidant activity;
 Cited by
Kwak IS. 2008. Comparison of different assays for evaluating antioxidant activity of polyphenols and tea extracts. PhD Dissertation. Chonbuk National University, Jeonju, Korea.

Halliwell B. 1991. Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med 91: 14S-22S.

Sies H. 1991. Oxidative stress: from basic research to clinical application. Am J Med 91: 31S-38S.

Ciencewicki J, Trivedi S, Kleeberger SR. 2008. Oxidants and the pathogenesis of lung diseases. J Allergy Clin Immunol 122: 456-468. crossref(new window)

Ames BN, Shigenaga MK, Hagen TM. 1993. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA 90: 7915-7922. crossref(new window)

Yang HS, Choi YJ, Oh HH, Moon JS, Jung HK, Kim KJ, Choi BS, Lee JW, Huh CK. 2014. Antioxidative activity of mushroom water extracts fermented by lactic acid bacteria. J Korean Soc Food Sci Nutr 43: 80-85. crossref(new window)

Yang YY, Kim JY, Kwon O. 2012. Development of flavonoid database for commonly consumed foods by Koreans. Korean J Nutr 45: 283-292. crossref(new window)

Holden JM, Bhagwat SA, Haytowitz DB, Gebhardt SE, Dwyer JT, Peterson J, Beecher GR, Eldridge AL, Balentine D. 2005. Development of a database of critically evaluated flavonoids data: application of USDA's data quality evaluation system. J Food Compos Anal 18: 829-844. crossref(new window)

Lim SH, Kim JK, Kim DH, Sohn SH, Lee JY, Kim YM, Ha SH. 2011. Flower color modification by manipulating flavonoid biosynthetic pathway. Kor J Hort Sci Technol 29:511-522.

Kim MJ, Ryu MJ. 2012. Inhibition of melanogenesis and anti-UV properties Reynoutria elliptica. Kor J Aesthet Cosmetol 10: 961-968.

Garcia-Alonso M, de Pascual-Teresa S, Santos-Buelga C, Rivas-Gonzalo JC. 2004. Evaluation of the antioxidant properties of fruits. Food Chem 84: 13-18. crossref(new window)

Lee SJ, Chung HY, Lee IK, Yoo ID. 1999. Isolation and identification of flavonoids from ethanol extracts of Artemisia vulgaris and their antioxidant activity. Korean J Food Sci Technol 31: 815-822.

Ismaili H, Sosa S, Brkic D, Fkih-Tetouani S, Ilidrissi A, Touati D, Aquino RP, Tubaro A. 2002. Topical anti-inflammatory activity of extracts and compounds from Thymus broussonettii. J Pharm Pharmacol 54: 1137-1140. crossref(new window)

Zhang WY, Lee JJ, Kim Y, Kim IS, Han JH, Lee SG, Ahn MJ, Jung SH, Myung CS. 2012. Effect of eriodictyol on glucose uptake and insulin resistance in vitro. J Agric Food Chem 60: 7652-7658. crossref(new window)

Bucolo C, Leggio GM, Drago F, Salomone S. 2012. Eriodictyol prevents early retinal and plasma abnormalities in streptozotocin- induced diabetic rats. Biochem Pharmacol 84: 88-92. crossref(new window)

Lee JH, Jhoo JW. 2012. Antioxidant activity of different parts of Lespedeza bicolor and isolation of antioxidant compound. Korean J Food Sci Technol 44: 763-771. crossref(new window)

Alia M, Ramos S, Mateos R, Granado-Serrano AB, Bravo L, Goya L. 2006. Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide. Toxicol Appl Pharmacol 212: 110-118. crossref(new window)

Molina MF, Sanchez-Reus I, Iglesias I, Benedi J. 2003. Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biol Pharm Bull 26: 1398-1402. crossref(new window)

Manju V, Nalini N. 2005. Chemopreventive potential of luteolin during colon carcinogenesis induced by 1,2-dimethylhydrazine. Ital J Biochem 54: 268-275.

Wong MC, Portmann B, Sherwood R, Niemela O, Koivisto H, Parkkila S, Trick K, L'abbe MR, Wilson J, Dash PR, Srirajaskanthan R, Preedy VR, Wiseman H. 2007. The cytoprotective effect of alpha-tocopherol and daidzein against d-galactosamine-induced oxidative damage in the rat liver. Metabolism 56: 865-875. crossref(new window)

Green RM, Flamm S. 2002. AGA technical review on the evaluation of liver chemistry tests. Gastroenterology 123: 1367-1384. crossref(new window)

Ahn SY, Park SY. 2012. A diagnostic approach to abnormal liver function tests in asymptomatic patients. Korean J Med 82: 134-142. crossref(new window)

Lim TS, Kim JS, Kim JM. 1984. Electrophoretic patterns of GGT, ALP and LDH isoenzyme in various hepatic diseases. Kyungpook Univ Med J 25: 268-285.

Yu KH. 2014. Effects of fermented aqueous extracts from Ligularia fischeri leaves by using lactic acid bacteria on improving liver function. PhD Dissertation. Kangwon National University, Chuncheon, Korea.

Kim KS, Jeon DS, Kim JS, Kim JM. 1982. GGT, ALPI and LDH in various liver diseases. Kyungpook Univ Med J 23: 325-333.

Kim KA. 2009. Understanding and application of liver function tests. Korean J Med 76: 163-168.

Choi K, Kim BI, Cho YK, Park CY, Sohn JI, Jeon WK, Kim H, Chung ES, Keum DG, Lee HY, Lee SJ. 1999. Diagnostic usefulness of serum ${\gamma}$-glutamyl transferase (GGT) activity in fatty liver and relationship with other factors. Korean J Med 57: 1006-1013.

Li H, Li C, Shen T, Zhao L, Ren D. 2014. R-eriodictyol and S-eriodictyol exhibited comparable effect against H2O2- induced oxidative stress in EA.hy926 cells. Drug Discov Ther 8: 214-224.

Areias FM, Rego AC, Oliveira CR, Seabra RM. 2001. Antioxidant effect of flavonoids after ascorbate/$Fe^{2+}$-induced oxidative stress in cultured retinal cells. Biochem Pharmacol 62: 111-118. crossref(new window)

Lee ER, Kim JH, Kang YJ, Cho SG. 2007. The anti-apoptotic and anti-oxidant effect of eriodictyol on UV-induced apoptosis in keratinocytes. Biol Pharm Bull 30: 32-37. crossref(new window)