JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Protective Effects of Ethanol Extract of Allium hookeri Root on Acute Alcohol-Induced Intoxication in ICR Mice
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Protective Effects of Ethanol Extract of Allium hookeri Root on Acute Alcohol-Induced Intoxication in ICR Mice
Kang, Hae-Young; Lee, Cho-Eun; Ly, Sun-Yung;
  PDF(new window)
 Abstract
Allium hookeri is known as a healthy food since it contains larger amounts of sulfur compounds than commonly known alliaceous plants. The antioxidant and anti-inflammatory effects of A. hookeri were compared between two types of extracts, water and 95% ethanol extracts of A. hookeri roots. A. hookeri root 95% ethanol extracts displayed superior total polyphenol content, antioxidant activity [1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) radical scavenging activity], and anti-inflammation activity than those of water extracts (P<0.05). We studied the effects of A. hookeri root 95% ethanol extracts (95% ethanol extracts group: AHE) on acute alcohol-induced intoxication in mice. AHE [250, 500, and 1,000 mg/kg body weight (BW)/d] was orally administered to the study group once a day for 1 week. On the last day of AHE treatment, 40% ethanol (10 mL/kg BW) was orally administered to induce acute liver injury. The blood alcohol concentration of mice treated with AHE was significantly lower compared to the control group (P<0.05). The levels of hepatic aspartate aminotransferase and alanine aminotransferase were lower in the AHE-treated group than the control group (P<0.05). The RT-PCR results for alcohol dehydrogenase and aldehyde dehydrogenase measured based on mRNA in liver tissues showed that enzyme activities were higher in the AHE-treated group than in the control group at a low blood alcohol concentration.
 Keywords
Allium hookeri;antioxidant and anti-inflammatory effect;acute alcohol-induced liver injury;
 Language
Korean
 Cited by
 References
1.
An SW, Kim YG, Kim MH, Lee BI, Lee SH, Kwon HI, Hwang B, Lee HY. 1999. Comparison of hepatic detoxification activity and reducing serum alcohol concentration of Hovenia dulsis THUNB and Alnus Japonica Steud. Korean J Med Crop Sci 7: 263-268.

2.
Korea National Health and Nutrition Examination Survey. 2013. https://knhanes.cdc.go.kr/knhanes/index.do (accessed Dec 2015).

3.
Lieber CS. 1997. Ethanol metabolism, cirrhosis and alcoholism. Clin Chim Acta 257: 59-84. crossref(new window)

4.
McGill HC. 1979. The relationship of dietary cholesterol to serum cholesterol concentration and to atherosclerosis in man. Am J Clin Nutr 32: 2664-2702.

5.
Berger K, Ajani UA, Kase CS, Gaziano JM, Buring JE, Glynn RJ, Hennekens CH. 1999. Light-to-moderate alcohol consumption and the risk of stroke among U.S. male physicians. N EngI J Med 341: 1557-1564. crossref(new window)

6.
Rimm EB, Williams P, Fosher K, Criqui M, Stampfer MJ. 1999. Moderate alcohol intake and lower risk of coronary heart disease: meta-analysis of effects on lipids and haemostatic factors. BMJ 319: 1523-1528. crossref(new window)

7.
Wheeler MD, Nakagami M, Bradford BU, Uesugi T, Mason RP, Connor HD, Dikalova A, Kadiiska M, Thurman RG. 2001. Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat. J Biol Chem 276: 36664-36672. crossref(new window)

8.
Mansouri A, Demeilliers C, Amsellem S, Pessayre D, Fromenty B. 2001. Acute ethanol administration oxidatively damages and depletes mitochondrial DNA in mouse liver, brain, heart, and skeletal muscles: protective effects of antioxidants. J Pharmacol Exp Ther 298: 737-743.

9.
Kim JS. 2004. Effect of a alcohol detoxification beverage (ADB) contained Radix puerariae and Bambusae caules in Liquamen Phyllostachyos on the alcohol administered mouse. J Korean Soc Food Sci Nutr 33: 318-323. crossref(new window)

10.
Rouach H, Clement M, Orfanelli MT, Janvier B, Nordmann J, Nordmann R. 1983. Hepatic lipid peroxidation and mitochondrial susceptibility to peroxidative attacks during ethanol inhalation and withdrawal. Biochem Biophys Acta 753: 439-444. crossref(new window)

11.
Moncade C, Torres V, Varghese G, Albano E, Israel Y. 1994. Ethanol-derived immunoreactive species formed by free radical mechanisms. Mol Pharmacol 46: 786-791.

12.
Simic MG. 1988. Mechanisms of inhibition of free-radical processes in mutagenesis and carcinogensis. Mutat Res 202: 377-386. crossref(new window)

13.
Block E. 1992. The organosulfur chemistry of the genus Allium - Implications for the organic chemistry of sulfur. Angew Chem Int Ed Engl 31: 1135-1178. crossref(new window)

14.
Corzo-Martinez M, Corzo N, Villamiel M. 2007. Biological properties of onions and garlic. Trends Food Sci Technol 18: 609-625. crossref(new window)

15.
Kim HJ, Chun HS. 1999. Biological functions of organosulfur compounds in Allium vegetables. J Korean Soc Food Sci Nutr 28: 1412-1423.

16.
You WC, Blot WJ, Chang YS, Ershow A, Yang ZT, An Q, Henderson BE, Fraumeni JF Jr, Wang TG. 1989. Allium vegetables and reduced risk of stomach cancer. J Natl Cancer Inst 81: 162-164. crossref(new window)

17.
Sparnins VL, Mott AW, Barany G, Wattenberg LW. 1986. Effects of allyl methyl trisulfide on glutathione S-transferase activity and BP-induced neoplasia in the mouse. Nutr Cancer 8: 211-215. crossref(new window)

18.
Sparnins VL, Barany G, Wattenberg LW. 1988. Effects of organosulfur compounds from garlic and onions on benzo [${\alpha}$]pyrene-induced neoplasia and glutathione S-transferase activity in the mouse. Carcinogenesis 9: 131-134. crossref(new window)

19.
Gudi VA, Singh SV. 1991. Effect of diallyl sulfide, a naturally occurring anti-carcinogen, on glutathione-dependent detoxification enzymes of female CD-1 mouse tissues. Biochem Pharmacol 42: 1261-1265. crossref(new window)

20.
Maurya AK, Singh SV. 1991. Differential induction of glutathione transferase isoenzymes of mice stomach by diallyl sulfide, a naturally occurring anticarcinogen. Cancer Lett 57: 121-129. crossref(new window)

21.
Schaffer EM, Liu JZ, Milner JA. 1997. Garlic powder and allyl sulfur compounds enhance the ability of dietary selenite to inhibit 7,12-dimethylbenz[${\alpha}$]anthracene-induced mammary DNA adducts. Nutr Cancer 27: 162-168. crossref(new window)

22.
Kim CH, Lee MA, Kim TW, Jang JY, Kim HJ. 2012. Antiinflammatory effect of Allium hookeri root methanol extract in LPS-induced RAW264.7 cells. J Korean Soc Food Sci Nutr 41: 1645-1648. crossref(new window)

23.
Hu JJ, Yoo JS, Lin M, Wang EJ, Yang CS. 1996. Protective effects of diallyl sulfide on acetaminophen-induced toxicities. Food Chem Toxicol 34: 963-969. crossref(new window)

24.
Lin MC, Wang EJ, Patten C, Lee MJ, Xiao F, Reuhl KR, Yang CS. 1996. Protective effect of diallyl sulfone against acetaminophen-induced hepatotoxicity in mice. J Biochem Toxicol 11: 11-20. crossref(new window)

25.
Wang BH, Zuzel KA, Rahman K, Billington D. 1998. Protective effects of aged garlic extract against bromobenzene toxicity to precision cut rat liver slices. Toxicology 126: 213-222. crossref(new window)

26.
Wang BH, Zuzel KA, Rahman K, Billington D. 1999. Treatment with aged garlic extract protects against bromobenzene toxicity to precision cut rat liver slices. Toxicology 132: 215-225. crossref(new window)

27.
Wang EJ, Li Y, Lin M, Chen L, Stein AP, Reuhl KR, Yang CS. 1996. Protective effects of garlic and related organosulfur compounds on acetaminophen-induced hepatotoxicity in mice. Toxicol Appl Pharmacol 136: 146-154. crossref(new window)

28.
Ayam VS. 2011. Allim hookeri, Thw. Enum. A lesser known terrestrial perennial herb used as food and its ethnobotanical relevance in manipur. Afr J Food Agric Nutr Dev 11: 5389-5412.

29.
Won JY, Yoo YC, Kang EJ, Yang H, Kim GH, Seong BJ, Kim SI, Han SH, Lee SS, Lee KS. 2013. Chemical components DPPH radical scavenging activity and inhibitory effects on nitric oxide production in Allium hookeri cultivated under open field and greenhouse conditions. J Korean Soc Food Sci Nutr 42: 1351-1356. crossref(new window)

30.
Kim CH, Lee MA, Kim TW, Jang JY, Kim HJ. 2012. Antiinflammatory effect of Allium hookeri root methanol extract in LPS-induced RAW264.7 cells. J Korean Soc Food Sci Nutr 41: 1645-1648. crossref(new window)

31.
Bae GC, Bae DY. 2012. The anti-inflammatory effects of ethanol extract of Allium Hookeri cultivated in South Korea. Kor J Herbology 27: 55-61.

32.
Park SY. 2014. A study on physicochemical and functional properties of Allium hookeri. MS Thesis. Chonbuk National University, Jeonju, Korea.

33.
Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.

34.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. crossref(new window)

35.
Pellegrini N, Re R, Yang M, Rice-Evans C. 1999. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenbenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Methods Enzymol 299: 379-389. crossref(new window)

36.
Jun HI, Park SY, Jeong DY, Song GS, Kim YS. 2014. Quality properties of yogurt added with hot water concentrates from Allium hookeri root. J Korean Soc Food Sci Nutr 43: 1415-1422. crossref(new window)

37.
Lee KW, Kim YS, Park PJ, Jeong JH. 2014. Comparison of effect of water and ethanolic extract from roots and leaves of Allium hookeri. J Korean Soc Food Sci Nutr 43: 1808-1816. crossref(new window)

38.
Kim KH, Kim HJ, Byun MW, Yook HS. 2012. Antioxidant and antimicrobial activities of ethanol extract from six vegetables containing different sulfur compounds. J Korean Soc Food Sci Nutr 41: 577-583. crossref(new window)

39.
Park MY, Ly SY. 2015. Quality characteristics of seasoned pork with water extracts of Allium hookeri root during storage. J Korean Soc Food Sci Nutr 44: 242-249. crossref(new window)

40.
Kang JR, Hwang CR, Sim HJ, Kang MJ, Kang ST, Shin JH. 2015. Biological activities of yellow garlic extract. J Korean Soc Food Sci Nutr 44: 983-992. crossref(new window)

41.
Dirsch VM, Kiemer AK, Wagner H, Vollmar AM. 1998. Effect of allicin and ajoene, two compounds of garlic, on inducible nitric oxide synthase. Atherosclerosis 139: 333-339. crossref(new window)

42.
Lee SR. 2002. A study on the alleviation effect of alcoholinduced hangover and antioxidative activity of Evodiae fructus. MS Thesis. Duksung Women's University, Seoul, Korea.

43.
Shin KH, Woo WS, Song YJ, Chung HS, Lee JM, Shin CS. 1995. Studies on the effect of Aloe spp. on ethanol metabolism (I) - effect of Aloe vera on serum ethanol level and hepatic ADH activity -. Kor J Pharmacogn 26: 148-153.

44.
Moon HI, Zee OP, Shin KH. 1998. Effect of perilla (Perilla frutescens Britton) extract on serum ethanol level and hepatic alcohol dehydrogenase activity. Korean J Med Crop Sci 6: 126-130.

45.
Park SH, Yoon HK, Koo SJ. 2004. Effects of water extracts of Camelia sinensis L on blood alcohol concentration and activities of acute alcohol metabolic enzymes in ICR mouse. J East Asian Soc Diet Life 14: 634-639.

46.
Park SM, Kang BK, Chung TH. 1998. The effect of mildronate on serum alcohol concentration and hangover syndrome. J Korean Soc Food Sci Nutr 27: 168-174.