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Physiological Activities and Inhibitory Effect of Extracts of Cynanchi wilfordii Radix and Perilla sikokiana against Cell Differentiation in 3T3-L1 Adipocytes
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 Title & Authors
Physiological Activities and Inhibitory Effect of Extracts of Cynanchi wilfordii Radix and Perilla sikokiana against Cell Differentiation in 3T3-L1 Adipocytes
Kim, Seon Jeong; Kang, Seung Mi; Ko, Keon Hee; Nam, Sanghae;
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Cynanchi wilfordii Radix (CW) and Perilla sikokiana (PS) were extracted under different conditions to study their antioxidant, anti-diabetic, and anti-obesity activities. Their potentials as functional food ingredients were investigated. The highest total phenol contents were for CW100 [100% fermented ethanol (FE) extract from CW] and for PS50 (50% FE extract from PS). When extracts were processed at 1 mg/mL, DPPH radical scavenging activities were and , respectively, at CW100 and PS50. ABTS radical scavenging activities were and , respectively, at CW100 and PS50. However, ferric reducing antioxidant power activities at 1 mg/mL were higher than 80% for PS under all extraction conditions. The highest -amylase inhibitory activities were at CW50 and at PS0. Cell differentiation inhibitory effects in 3T3-L1 adipocytes were at CW100 and at PS50. The inhibitory effect of the CW100-PS50 mixture was , which was significantly higher than those of individual extracts.
Cynanchi wilfordii;Perilla sikokiana;antioxidant;-amylase activity;3T3-L1 adipocyte cell;
 Cited by
Lee WC, Kim AJ, Kim SY. 2003. The study on the functional materials and effects of mulberry leaf. Food Science and Industry 36(3): 2-14.

Kim SY, Ryu KS, Lee WC, Ku HO, Lee HS, Lee KR. 1999. Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice. Kor J Pharmacogn 30: 123-129.

Bauer G. 2000. Reactive oxygen and nitrogen species: efficient, selective, and interactive signals during intercellular induction of apoptosis. Anticancer Res 20: 4115-4139.

Branen AL. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc 52: 59-63. crossref(new window)

Omaye ST, Reddy KA, Cross CE. 1977. Effect of butylated hydroxytoluene and other antioxidants on mouse lung metabolism. J Toxicol Environ Health 3: 829-836. crossref(new window)

Cho YJ, Ju IS, Chun SS, An BJ, Kim JH, Kim MU, Kwon OJ. 2008. Screening of biological activities of extracts from Rhododendron mucronulatum Turcz. flowers. J Korean Soc Food Sci Nutr 37: 276-281. crossref(new window)

Wen KC, Huang CY, Lu FL. 1993. Determination of baicalin and puerarin in traditional Chinese medicinal preparations by high-performance liquid chromatography. J Chromatogr A 631: 241-250. crossref(new window)

Wang H, Zou H, Ni J, Kong L, Gao S, Guo B. 2000. Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase. J Chromatogr A 870: 501-510. crossref(new window)

Choi H, Zhu M, Kim C, Lee J. 2003. Studies of name and herbal origins of Ha-Soo-Oh. Korean J Orient Med 9: 81-89.

Kim HK, Kim YA, Lee AY, Ko BS. 2003. Pattern analysis of Cynanchi Wilfordi Radix and Polygoni Multiflori Radix. Kor J Pharmacogn 34: 278-281.

Lee TB. 1993. Illustrated guide to Korean flora. Hyangmunsa, Seoul, Korea. p 304.

Kim HS. 2004. Effects of Cynanchum wilfordii extract on serum lipid components and enzyme activities in hyperlipidemic and streptozotocin-induced diabetic rats. Korean J Hum Ecol 7: 1-11.

Han DS, Chung BH, Yoo HG, Kim YO, Beak SH. 1994. Studies on the cytotoxicity and antitumor activity of Perilla frutescens. Kor J Pharmacogn 25: 249-257.

Jang SM, Noh SH, Park SD. 1999. Botany of herbal resource. Hakmun Publishing Co., Seoul, Korea. p 473-476.

Noh SH, Kim SH, Lee KH, Ahn DK, Lee YJ, Kang BS, Ko WC, Song HJ, Joo YS. 1991. Medicinal plants. Young Lim Publishing Co., Seoul, Korea. p 125-128.

Son HU, Heo JC, Seo MS, Lee SH. 2010. Effects of Perilla frutescens L. on anti-oxidant and anti-inflammation activity. Korean J Food Preserv 17: 757-761.

Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.

Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. crossref(new window)

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. crossref(new window)

Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem 239: 70-76. crossref(new window)

Gao H, Kawabata J. 2005. ${\alpha}$-Glucosidase inhibition of 6-hydroxyflavones. Part 3: Synthesis and evaluation of 2,3,4-trihydroxybenzoyl-containing flavonoid analogs and 6-aminoflavones as ${\alpha}$-glucosidase inhibitors. Bioorg Med Chem 13: 1661-1671. crossref(new window)

Hwang JT, Park IJ, Shin JI, Lee YK, Lee SK, Baik HW, Ha J, Park OJ. 2005. Genistein, EGCG, and capsaicin inhibit adipocyte differentiation process via activating AMP-activated protein kinase. Biochem Biophys Res Commun 338: 694-699. crossref(new window)

Sargent JM, Taylor CG. 1989. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeloid leukaemia. Br J Cancer 60: 206-210. crossref(new window)

Yoshizawa S, Horiuchi T, Fujiki H, Yoshida T, Okuda T, Sugimura T. 1987. Antitumor promoting activity of (-)-epigallocatechin gallate, the main constituent of "tannin" in green tea. Phytother Res 1: 44-47. crossref(new window)

Oboh G, Akindahunsi AA. 2004. Change in the ascorbic acid, total phenol and antioxidant activity of sun-dried commonly consumed green leafy vegetables in Nigeria. Nutr Health 18: 29-36. crossref(new window)

Jeong HJ, Park SB, Kim S, Kim HK. 2007. Total polyphenol content and antioxidative activity of Wild Grape (Vitis coignetiae) extracts depending on ethanol concentrations. J Korean Soc Food Sci Nutr 36: 1491-1496. crossref(new window)

Lee SJ, Bang WS, Hong JY, Kwon OJ, Shin SR, Yoon KY. 2013. Antioxidant and antimicrobial activities of black Doraji (Platycodon grandiflorum). Korean J Food Preserv 20: 510-517. crossref(new window)

Ancerewicz J, Migliavacca E, Carrupt PA, Testa B, Bree F, Zini R, Tillement JP, Labidalle S, Guyot D, Chauvet-Monges AM, Crevet A, Le Ridant A. 1998. Structure-property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radic Biol Med 25: 113-120. crossref(new window)

Kim JM, Cho ML, Seo KE, Kim YS, Jung TD, Kim YH, Kim DB, Shin GH, Oh JW, Lee JS, Lee JH, Kim JY, Lee DW, Lee OH. 2015. Effect of extraction conditions on in vitro antioxidant activities of root bark extract from Ulmus pumila L.. J Korean Soc Food Sci Nutr 44: 1172-1179. crossref(new window)

Kim JM, Kim DJ, Kim TH, Kim HS, Choe M. 2010. Antioxidative and anti-inflammatory effect of water extract from Perillae semen in RBL-2H3 cells. Korean J Nutr 43: 367-373. crossref(new window)

Kim YE, Yang JW, Lee CH, Kwon EK. 2009. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake Sing. (pine mushroom). J Korean Soc Food Sci Nutr 38: 555-560. crossref(new window)

Park EJ, Ahn JJ, Kwon JH. 2013. Effect of reflux conditions on extraction properties and antioxidant activity of freeze dried-Schisandra chinensis. Korean J Food Sci Technol 45: 550-556. crossref(new window)

Lee BB, Park SR, Han CS, Han DY, Park E, Park HR, Lee SC. 2008. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr 37: 405-409. crossref(new window)

Oh S, Hong SS, Kim YH, Koh SC. 2008. Screening of biological activities in fern plants native to Jeju Island. Korean J Plant Res 21: 12-18.

Jeong HJ, Lee SG, Lee EJ, Park WD, Kim JB, Kim HJ. 2010. Antioxidant activity and anti-hyperglycemic activity of medicinal herbal extracts according to extraction methods. Korean J Food Sci Technol 42: 571-577.

Park SJ, Lee IS, Lee SP, Yu MH. 2013. Inhibition of adipocyte differentiation and adipogenesis by supercritical fluid extracts and marc from Cinnamomum verum. J Life Sci 23: 510-517. crossref(new window)

McNeel RL, Mersmann HJ. 2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and differentiation. J Nutr Biochem 14: 266-274. crossref(new window)