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Sorghum Extract Lowers Lymphatic Absorption of Trans Fat and Cholesterol in Rats
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 Title & Authors
Sorghum Extract Lowers Lymphatic Absorption of Trans Fat and Cholesterol in Rats
Kim, Juyeon; Noh, Sang Kyu; Woo, Koan-Sik; Seo, Myung-Chul;
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 Abstract
Excessive intake of trans fats is a risk factor for cardiovascular diseases. Previously, we showed that green tea extract lowers intestinal absorption of cholesterol and elaidic acid in rats. This study was conducted to investigate a possible role for sorghum extract on lymphatic absorption of trans fat and cholesterol in rats. Adult male rats with lymph cannulae were infused at a rate of 3.0 mL/h for 8 h via a duodenal catheter with a lipid emulsion containing of trielaidin, of trilinoelaidin, of triolein, cholesterol labeled with (), of cholesterol, and of Na-taurocholate without or with 100.0 mg of sorghum extract in phosphate-buffered saline buffer (pH 6.4). Lymph was collected hourly for 8 h. No significant difference was noted in lymph flow. However, the lymphatic absorption of elaidic acid and linoelaidic acid for 8 h was significantly lower in rats infused with sorghum extract than in those infused with no sorghum extract. Further, lymphatic absorption of was reduced by sorghum extract, which was observed previously. These data indicate that sorghum extract has an inhibitory effect on the intestinal absorption of trans fat and cholesterol. The mechanism(s) by which sorghum extract lowers intestinal absorption of trans fat warrants further study.
 Keywords
cholesterol;intestinal absorption;rats;sorghum extract;trans fat;
 Language
Korean
 Cited by
 References
1.
Semma M. 2002. Trans fatty acids: properties, benefits and risks. J Health Sci 48: 7-13. crossref(new window)

2.
Zbikowska A. 2010. Formation and properties of trans fatty acids - a review. Pol J Food Nutr Sci 60: 107-114.

3.
Lopez-Garcia E, Schulze MB, Meigs JB, Manson JE, Rifai N, Stampfer MJ, Willett WC, Hu FB. 2005. Consumption of trans fatty acids is related to plasma biomarkers of inflammation and endothelial dysfunction. J Nutr 135: 562-566.

4.
Bray GA, Lovejoy JC, Smith SR, DeLany JP, Lefevre M, Hwang D, Ryan DH, York DA. 2002. The influence of different fats and fatty acids on obesity, insulin resistance and inflammation. J Nutr 132: 2488-2491.

5.
Hammad S, Pu S, Jones PJ. 2016. Current evidence supporting the link between dietary fatty acids and cardiovascular disease. Lipids 51: 501-517.

6.
Lichtenstein AH. 2014. Dietary trans fatty acids and cardiovascular disease risk: past and present. Curr Atheroscler Rep 16: 433. crossref(new window)

7.
Nestel P. 2014. Trans fatty acids: are its cardiovascular risks fully appreciated?. Clin Ther 36: 315-321. crossref(new window)

8.
Chavarro JE, Furtado J, Toth TL, Ford J, Keller M, Campos H, Hauser R. 2011. Trans-fatty acid levels in sperm are associated with sperm concentration among men from an infertility clinic. Fertil Steril 95: 1794-1797. crossref(new window)

9.
Grimm MO, Rothhaar TL, Grosgen S, Burg VK, Hundsdorfer B, Haupenthal VJ, Friess P, Kins S, Grimm HS, Hartmann T. 2012. Trans fatty acids enhance amyloidogenic processing of the Alzheimer amyloid precursor protein (APP). J Nutr Biochem 23: 1214-1223. crossref(new window)

10.
Hu J, La Vecchia C, de Groh M, Negri E, Morrison H, Mery L. 2011. Dietary transfatty acids and cancer risk. Eur J Cancer Prev 20: 530-538. crossref(new window)

11.
de Souza RJ, Mente A, Maroleanu A, Cozma AI, Ha V, Kishibe T, Uleryk E, Budylowski P, Schunemann H, Beyene J, Anand SS. 2015. Intake of saturated and trans unsaturated fatty acids and risk of all cause mortality, cardiovascular disease, and type 2 diabetes: systematic review and metaanalysis of observational studies. BMJ 351: h3978.

12.
Ryu HS, Kim J, Kim HS. 2006. Enhancing effect of Sorghum bicolor L. Moench (sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Korean J Food & Nutr 19: 176-182.

13.
Waniska RD, Poe JH, Bandyopadhyay R. 1989. Effects of growth conditions on grain molding and phenols in sorghum caryopsis. J Cereal Sci 10: 217-225. crossref(new window)

14.
Castano G, Menendez R, Mas R, Amor A, Fernandez JL, Gonzalez RL, Lezcay M, Alvarez E. 2002. Effects of policosanol and lovastatin on lipid profile and lipid peroxidation in patients with dyslipidemia associated with type 2 diabetes mellitus. Int J Clin Pharmacol Res 22: 89-99.

15.
Carr TP, Weller CL, Schlegel VL, Cuppett SL, Guderian DM Jr, Johnson KR. 2005. Grain sorghum lipid extract reduces cholesterol absorption and plasma non-HDL cholesterol concentration in hamsters. J Nutr 135: 2236-2240.

16.
Cho SH, Ha TY. 2003. In vitro and in vivo effects of prosomillet and sorghum on cholesterol metabolism. Food Sci Biotechnol 12: 485-490.

17.
Klopfenstein CF, Varriano-Marston E, Hoseney RC. 1981. Cholesterol-lowering effect of sorghum diet in guinea pigs. Nutr Rep Int 24: 621-627.

18.
Al-Mamary M, Molham AH, Abdulwali AA, Al-Obeidi A. 2001. In vivo effects of dietary sorghum tannins on rabbit digestive enzymes and mineral absorption. Nutr Res 21: 1393-1401. crossref(new window)

19.
Koo SI, Noh SK. 2007. Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipidlowering effect. J Nutr Biochem 18: 179-183. crossref(new window)

20.
Ikeda I, Imasato Y, Sasaki E, Nakayama M, Nagao H, Takeo T, Yayabe F, Sugano M. 1992. Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. Biochim Biophys Acta 1127: 141-146. crossref(new window)

21.
Loest HB, Noh SK, Koo SI. 2002. Green tea extract inhibits the lymphatic absorption of cholesterol and ${\alpha}$-tocopherol in ovariectomized rats. J Nutr 132: 1282-1288.

22.
Kim J, Koo SI, Noh SK. 2012. Green tea extract markedly lowers the lymphatic absorption and increases the biliary secretion of $^{14}C$-benzo[a]pyrene in rats. J Nutr Biochem 23: 1007-1011. crossref(new window)

23.
Seo MC, Ko JY, Song SB, Lee JS, Kang JR, Kwak DY, Oh BG, Yoon YN, Nam MH, Jeong HS, Woo KS. 2011. Antioxidant compounds and activities of foxtail millet, proso millet and sorghum with different pulverizing methods. J Korean Soc Food Sci Nutr 40: 790-797. crossref(new window)

24.
Bollman JL, Cain JC, Grindlay JH. 1948. Techniques for the collection of lymph from the liver, small intestine, or thoracic duct of the rat. J Lab Clin Med 33: 1349-1352.

25.
Koo SI, Noh SK. 2001. Phosphatidylcholine inhibits and lysophosphatidylcholine enhances the lymphatic absorption of ${\alpha}$-tocopherol in adult rats. J Nutr 131: 717-722.

26.
Folch J, Lees M, Sloane-Stanley GH. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226: 497-509.

27.
Slover HT, Lanza E. 1979. Quantitative analysis of food fatty acids by capillary gas chromatography. J Am Oil Chem Soc 56: 933-943. crossref(new window)

28.
Tso P, Balint JA. 1986. Formation and transport of chylomicrons by enterocytes to the lymphatics. Am J Physiol 250: G715-G726.

29.
Juhel C, Armand M, Pafumi Y, Rosier C, Vandermander J, Lairon D. 2000. Green tea extract (AR25) inhibits lipolysis of triglycerides in gastric and duodenal medium in vitro. J Nutr Biochem 11: 45-51. crossref(new window)

30.
Lindahl M, Tagesson C. 1997. Flavonoids as phospholipase $A_2$ inhibitors: importance of their structure for selective inhibition of group II phospholipase $A_2$. Inflammation 21: 347-356. crossref(new window)

31.
Wang S, Noh SK, Koo SI. 2006. Green tea catechins inhibit pancreatic phospholipase $A_2$ and intestinal absorption of lipids in ovariectomized rats. J Nutr Biochem 17: 492-498. crossref(new window)

32.
Reynier MO, Lafont H, Crotte C, Sauve P, Gerolami A. 1985. Intestinal cholesterol uptake: comparison between mixed micelles containing lecithin or lysolecithin. Lipids 20: 145-150. crossref(new window)

33.
Wang S, Noh SK, Koo SI. 2006. Epigallocatechin gallate and caffeine differentially inhibit the intestinal absorption of cholesterol and fat in ovariectomized rats. J Nutr 136: 2791-2796.

34.
Yoo YS, Lee KS, Cho KH, Kim DH, Park CH, Lee SH, Lee JH. 2009. Effect of barley on variation of blood glucose and lipid metabolism. Korean J Fam Med 30: 790-795. crossref(new window)

35.
Kim J, Noh SK. 2012. Green tea extract decreases the lymphatic absorption of trans fat in rats. J Korean Soc Food Sci Nutr 41: 73-78. crossref(new window)