Advanced SearchSearch Tips
In Vitro Hepatoprotective Effects of Fermented Curcuma longa L. by Aspergillus oryzae against Alcohol-Induced Oxidative Stress
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
In Vitro Hepatoprotective Effects of Fermented Curcuma longa L. by Aspergillus oryzae against Alcohol-Induced Oxidative Stress
Sung, Heami; Lee, Yoo-Hyun; Jun, Woojin;
  PDF(new window)
Protective effects of fermented Curcuma longa L. (CL) against alcoholic liver damage were investigated in HepG2/2E1 cells. Fermented CL was extracted by cold water (FCC), hot water, 80% ethanol, and methanol. Of the four extracts, the strongest hepatoprotective effect against ethanol-induced oxidative stress was observed in FCC. Pretreatment with FCC also reduced intracellular reactive oxygen species formation compared to ethanol-alone treated cells. FCC also enhanced catalase, glutathione-S-transferase, glutathione reductase, glutathione peroxidase, and superoxide dismutase, and non-enzymatic antioxidative activities such as glutathione compared to alcohol-treated HepG2/2E1 cells. Our findings suggest that FCC might be considered as a useful agent in the prevention of liver damage induced by oxidative stress by increasing the antioxidant defense mechanism.
alcohol;oxidative damage;hepatoprotective effect;HepG2/2E1;Curcuma longa L.;
 Cited by
Hwang JY, Ham JW, Nam SH. 2004. Effect of Maesil (Prunus mume) juice on the alcohol metabolizing enzyme activities. Korean J Food Sci Technol 36: 329-332.

Lin RC, Li TK. 1998. Effects of isoflavones on alcohol pharmacokinetics and alcohol-drinking behavior in rats. Am J Clin Nutr 68: 1512S-1515S. crossref(new window)

Hawkins RD, Kalant H. 1972. The metabolism of ethanol and its metabolic effects. Pharmacol Rev 24: 67-157.

Chung YI, Bae IY, Lee JY, Chun HS, Lee HG. 2009. Protective effects of branched-chain amino acid (BCAA)-enriched corn gluten hydrolysates on ethanol-induced hepatic injury in rats. Korean J Food Sci Technol 41: 706-711.

Zakhari S. 2006. Overview: how is alcohol metabolized by the body?. Alcohol Res Health 29: 245-254.

Rouach H, Fataccioli V, Gentil M, French SW, Morimoto M, Nordmann R. 1997. Effect of chronic ethanol feeding on lipid peroxidation and protein oxidation in relation to liver pathology. Hepatology 25: 351-355. crossref(new window)

French SW, Wong K, Jui L, Albano E, Hagbjork AL, Ingelman-Sundberg M. 1993. Effect of ethanol on cytochrome P450 2E1 (CYP2E1), lipid peroxidation, and serum protein adduct formation in relation to liver pathology pathogenesis. Exp Mol Pathol 58: 61-75. crossref(new window)

Zelko IN, Mariani TJ, Folz RJ. 2002. Superoxide dismutase multigene family: a comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radic Biol Med 33: 337-349. crossref(new window)

Chang P, Cheng E, Brooke S, Sapolsky R. 2005. Marked differences in the efficacy of post-insult gene therapy with catalase versus glutathione peroxidase. Brain Res 1063: 27-31. crossref(new window)

Kang SK. 2007. Changes in proximate composition, free amino acid, free sugar and vitamin of Curcuma longa L. and Curcuma atomatica Salib according to picking time. Korean J Food Preserv 14: 624-632.

Jung SH, Chang KS, Ko KH. 2004. Physiological effects of curcumin extracted by supercritical fluid from turmeric (Curcuma longa L.). Korean J Food Sci Technol 36: 317-320.

Eun CS. 2015. A study on the memory and cognitive enhancing effect of fermented turmeric. MS Thesis. Keimyung University, Daegu, Korea.

Kim HJ, Lee JW, Kim YD. 2011. Antimicrobial activity and antioxidant effect of Curcuma longa, Curcuma aromatica and Curcuma zedoaria. Korean J Food Preserv 18: 219-225. crossref(new window)

Kim MS, Chun SS, Choi JH. 2013. Effects of turmeric (Curcuma longa L.) on antioxidative systems and oxidative damage in rats fed a high fat and cholesterol diet. J Korean Soc Food Sci Nutr 42: 570-576. crossref(new window)

Kim Y, You Y, Jun W. 2012. Hepatoprotective activity of fermented Curcuma longa L. on galactosamine-intoxicated rats. J Korean Soc Food Sci Nutr 41: 790-795. crossref(new window)

Ha Y, Kim OK, Nam DE, Kim Y, Kim E, Jun W, Lee J. 2015. Effects of Curcuma longa L. extracts on natural killer cells and T cells. J Korean Soc Food Sci Nutr 44: 307-313. crossref(new window)

Shehzad A, Ul-lslam M, Wahid F, Lee YS. 2014. Multifunctional polymeric nanocurcumin for cancer therapy. J Nanosci Nanotechnol 14: 803-814. crossref(new window)

Kang SK, Hyun KH. 2007. Optimization of curcumin extraction and removal of bitter substance from Curcuma longa L.. Korean J Food Preserv 14: 722-726.

Lim YM, Kim BR, Hong KY. 2008. Antioxidant effect of Crataegi Fructus extract on the oxidative stress of reactive oxygen species in cultured human skin fibroblast. Korean J Orient Physiol Pathol 22: 115-119.

Aebi H. 1984. Catalase in vitro. Methods Enzymol 105: 121-126. crossref(new window)

McCord JM, Fridovich I. 1969. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244: 6049-6055.

Habig WH, Jakoby WB. 1981. Assays for differentiation of glutathione S-transferases. Methods Enzymol 77: 398-405. crossref(new window)

Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158-169.

Carlberg I, Mannervik B. 1975. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem 250: 5475-5480.

Akerboom TPM, Sies H. 1981. Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol 77: 373-382. crossref(new window)

Draper HH, Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 186: 421-431. crossref(new window)

Eliasson E, Johansson I, Ingelman-Sundberg M. 1988. Ligand-dependent maintenance of ethanol-inducible cytochrome P-450 in primary rat hepatocyte cell cultures. Biochem Biophys Res Commun 150: 436-443. crossref(new window)

Yang MX, Cederbaum AI. 1997. Glycerol increases content and activity of human cytochrome P-4502E1 in a transduced HepG2 cell line by protein stabilization. Alcohol Clin Exp Res 21: 340-347. crossref(new window)

Lee GH, Yoo DY. 2012. Evaluation of anti-inflammatory effects of Yukmijihwangtang and individual drug substances based on the extraction methods. J Orient Obstet Gynecol 25: 89-107.

Jang JH, Lee C, Kim SC, Chung JW, Park CI. 2010. Protective effect of marine natural products against UVB-induced damages in human skin fibroblast via antioxidant mechanism. J Soc Cosmet Sci Korea 36: 79-87.

Bae D, Kim J, Lee SY, Choi EJ, Jung MA, Jeong CS, Na JR, Kim JJ, Kim S. 2015. Hepatoprotective effects of aqueous extracts from leaves of Dendropanax morbifera Leveille against alcohol-induced hepatotoxicity in rats and in vitro anti-oxidant effects. Food Sci Biotechnol 24: 1495-1503. crossref(new window)

Yoo Y, Yoo S, Yoon HG, Park J, Lee YH, Kim S, Oh KT, Lee J, Cho HY, Jun W. 2010. In vitro and in vivo hepatoprotective effects of the aqueous extract from Taraxacum officinale (dandelion) root against alcohol-induced oxidative stress. Food Chem Toxicol 48: 1632-1637. crossref(new window)

Kim OK, Ho JN, Nam DE, Jun W, Hwang KT, Kang JE, Chae OS, Lee J. 2012. Hepatoprotective effect of Curdrania tricuspidata extracts against oxidative damage. J Korean Soc Food Sci Nutr 41: 7-13. crossref(new window)

Hong S, Seo W, Jung H, Kang S. 1998. Protecting effects by rooibos tea against immobilization stress-induced cellular damage in rat. Korean J Food Sci Technol 30: 1222-1228.

Kang DG, Yun CK, Lee HS. 2003. Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea. J Ethnopharmacol 87: 231-236. crossref(new window)

Ahn TH, Yang YS, Lee JC, Moon CJ, Kim SH, Jun W, Park SC, Kim JC. 2007. Ameliorative effects of $pycnogenol^{(R)}$ on carbon tetrachloride-induced hepatic oxidative damage in rats. Phytother Res 21: 1015-1019. crossref(new window)

Paradis V, Kollinger M, Fabre M, Holstege A, Poynard T, Bedossa P. 1997. In situ detection of lipid peroxidation byproducts in chronic liver diseases. Hepatology 26: 135-142. crossref(new window)

Yeon SH, Ham H, Sung J, Kim Y, Namkoong S, Jeong HS, Lee J. 2013. Antioxidant activities of hot water extract from Cornus walteri Wanger against oxidative stress induced by tert-butyl hydroperoxide in HepG2 cells. J Korean Soc Food Sci Nutr 42: 1525-1532. crossref(new window)

You Y, Jung KY, Lee YH, Jun W, Lee BY. 2009. Hepatoprotective effects of Hovenia dulcis fruit on ethanol-induced liver damage in vitro and in vivo. J Korean Soc Food Sci Nutr 38: 154-159. crossref(new window)

Oh SY, Seo SH, Lee JH, Lee JS, Ma JY. 2011. Protective effect of Galgeun-tang against $CCl_4$ induced hepatotoxicity. Korean J Orient Physiol Pathol 25: 663-668.