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Antioxidant and Anti-Proliferative Activities of Oats under Different Solvent Extraction Conditions
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 Title & Authors
Antioxidant and Anti-Proliferative Activities of Oats under Different Solvent Extraction Conditions
Ham, Hyeonmi; Woo, Koan Sik; Park, Ji-Young; Lee, Byongwon; Choi, Yong-Hwan; Lee, Choonwoo; Kim, Wook Han; Lee, Junsoo; Lee, Yu-Young;
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The objective of this study was to determine the antioxidant and anti-proliferative activities of methanol, ethanol, acetone, and ethyl acetate extracts from oats (Avena sativa L.). Total polyphenol contents of extracts were analyzed by Folin-Ciocalteu assay. The antioxidant activities of extracts were determined by 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and reducing power. The anti-proliferative activities of colon (HCT116), lung (NCI-H460), and breast (MCF7) cancer cells were investigated. Among solvents, methanol extract showed the highest amount of total polyphenols, which was 8.2 mg gallic acid equivalents/g residue. High levels of ABTS radical [12.1 mg Trolox equivalent antioxidant capacity (TEAC)/g residue] and DPPH radical (4.4 mg TEAC/g residue) scavenging activity and reducing power () were found in methanol extracts. Moreover, methanol extracts indicated higher anti-proliferative activities against HCT116 (69.5%), NCI-H460 (75.2%), and MCF7 (84.8%) cells compared with other extracts. The results show that methanol was the best solvent for extraction of antioxidant and anti-proliferative compounds from oats. Moreover, notable antioxidant and anti-proliferative activities of oats could have significant health benefits.
 Cited by
귀리 도정 부위별 메탄올 추출물의 항산화 성분 및 항산화 활성,함현미;우관식;박지영;이병원;최혜선;최용환;이준수;이유영;

한국식품영양과학회지, 2016. vol.45. 11, pp.1681-1684 crossref(new window)
Ha TY. 2006. Development of functional food materials for healthy life. Korean J Crop Sci 51: 26-39.

Kim JH, Kim MY. 2015. Anticancer effect of citrus fruit prepared by gamma irradiation of budsticks. J Life Sci 25: 1051-1058. crossref(new window)

Torre L, Siegel R, Jemal A. 2015. Global cancer facts & figures. 3rd ed. American Cancer Society Inc., Atlanta, GA, USA.

Choi JH, Kim HI, Lee IS. 2009. Effect of Rosmarinus officinalis L. on growth inhibition and apoptosis induction in cancer cells. J Korean Soc Food Sci Nutr 38: 1008-1015. crossref(new window)

Biesalski HK. 2002. Free radical theory of aging. Curr Opin Clin Nutr Metab Care 5: 5-10. crossref(new window)

Stadtman ER, Berlett BS. 1998. Reactive oxygen-mediated protein oxidation in aging and disease. Drug Metab Rev 30: 225-243. crossref(new window)

Neergheen VS, Bahorun T, Taylor EW, Jen LS, Aruoma OI. 2010. Targeting specific cell signaling transduction pathways by dietary and medicinal phytochemicals in cancer chemoprevention. Toxicology 278: 229-241. crossref(new window)

Jeong YS, Kim JW, Lee ES, Gil NY, Kim SS, Hong ST. 2014. Optimization of alkali extraction for preparing oat protein concentrates from oat groat by response surface methodology. J Korean Soc Food Sci Nutr 43: 1462-1466. crossref(new window)

Aman P, Graham H. 1987. Analysis of total and insoluble mixed-linked (1$\rightarrow$3),(1$\rightarrow$4)-${\beta}$-D-glucans in barley and oats. J Agric Food Chem 35: 704-709. crossref(new window)

Boz H. 2015. Phenolic amides (avenanthramides) in oats - a review. Czech J Food Sci 33: 399-404.

Davidson MH, Dugan LD, Burns JH, Bova J, Story K, Drennan KB. 1991. The hypocholesterolemic effects of ${\beta}$-glucan in oatmeal and oat bran. A dose-controlled study. JAMA 265: 1833-1839. crossref(new window)

Berg A, Konig D, Deibert P, Grathwohl D, Berg A, Baumstark MW, Franz IW. 2003. Effect of an oat bran enriched diet on the atherogenic lipid profile in patients with an increased coronary heart disease risk. A controlled randomized lifestyle intervention study. Ann Nutr Metab 47: 306-311. crossref(new window)

Tong L, Liu L, Zhong K, Wang Y, Guo L, Zhou S. 2014. Effects of cultivar on phenolic content and antioxidant activity of naked oat in China. J Integr Agric 13: 1809-1816. crossref(new window)

Tapola N, Karvonen H, Niskanen L, Mikola M, Sarkkinen E. 2005. Glycemic responses of oat bran products in type 2 diabetic patients. Nutr Metab Cardiovasc Dis 15: 255-261. crossref(new window)

Chang HC, Huang CN, Yeh DM, Wang SJ, Peng CH, Wang CJ. 2013. Oat prevents obesity and abdominal fat distribution, and improves liver function in humans. Plant Foods Hum Nutr 68: 18-23. crossref(new window)

Peng CH, Chang HC, Yang MY, Huang CN, Wang SJ, Wang CJ. 2013. Oat attenuate non-alcoholic fatty liver and obesity via inhibiting lipogenesis in high fat-fed rat. J Funct Foods 5: 53-61. crossref(new window)

Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46: 4113-4117. crossref(new window)

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. crossref(new window)

Sharma OP, Bhat TK. 2009. DPPH antioxidant assay revisited. Food Chem 113: 1202-1205. crossref(new window)

Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared form glucosamine. Jpn J Nutr 44: 307-315. crossref(new window)

Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63. crossref(new window)

Tatiya AU, Tapadiya GG, Kotecha S, Surana SJ. 2011. Effect of solvents on total phenolics, antioxidant and antimicrobial properties of Bridelia retusa Spreng. stem bark. Indian J Nat Prod Resour 2: 442-447.

Stoner GD, Mukhtar H. 1995. Polyphenols as cancer chemopreventive agents. J Cell Biochem Suppl 22: 169-180.

Duthie GG, Duthie SJ, Kyle JAM. 2000. Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutr Res Rev 13: 79-106. crossref(new window)

Choupani M, Delouee SA, Alami M. 2014. Antioxidant properties of various solvent extracts of lemon verbena (Lippia citriodora) leaves. Int J Adv Biol Biomed Res 2: 1340-1346.

Oki T, Masuda M, Kobayashi M, Nishiba Y, Furuta S, Suda I, Sato T. 2002. Polymeric procyanidins as radical-scavenging components in red-hulled rice. J Agric Food Chem 50: 7524-7529. crossref(new window)

Sultana B, Anwar F, Ashraf M. 2009. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14: 2167-2180. crossref(new window)

Ham H, Woo KS, Lee B, Park JY, Sim EY, Kim BJ, Lee C, Kim SJ, Kim WH, Lee J, Lee YY. 2015. Antioxidant compounds and activities of methanolic extracts from oat cultivars. J Korean Soc Food Sci Nutr 44: 1660-1665. crossref(new window)

Guo W, Nie L, Wu D, Wise ML, Collins FW, Meydani SN, Meydani M. 2010. Avenanthramides inhibit proliferation of human colon cancer cell lines in vitro. Nutr Cancer 62: 1007-1016. crossref(new window)

Meydani M. 2009. Potential health benefits of avenanthramides of oats. Nutr Rev 67: 731-735. crossref(new window)