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Behavior of Campylobacter jejuni Biofilm Cells and Viable But Non-Culturable (VBNC) C. jejuni on Smoked Duck
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 Title & Authors
Behavior of Campylobacter jejuni Biofilm Cells and Viable But Non-Culturable (VBNC) C. jejuni on Smoked Duck
Jo, Hye Jin; Jeon, Hye Ri; Yoon, Ki Sun;
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Biofilm cells and viable but non-culturable (VBNC) state may play a role in the survival of Campylobacter jejuni under unfavorable environmental conditions. The objective of this study was to investigate the behavior of C. jejuni biofilm cells and VBNC cells on smoked duck. The transfer of C. jejuni biofilm cells to smoked duck and its ability to resuscitate from biofilm and VBNC cells on smoked duck was investigated. Transfer experiments were conducted from C. jejuni biofilm cells to smoked duck after 5 min, 1 h, 3 h, and 24 h contact at room temperature, and the efficiency of transfer (EOT) was calculated. In addition, smoked duck was inoculated with C. jejuni biofilm and VBNC cells and then stored at 10, 24, 36, and to examine the cells' ability to resuscitate on smoked ducks. The 5 min contact time between C. jejuni biofilm cells and smoked duck showed a higher EOT (0.92) than the 24 h contact time (EOT=0.08), and the EOT decreased as contact time increased. Furthermore, C. jejuni biofilm cells on smoked duck were not recovered at 10, 24, and , and C. jejuni VBNC cells were not resuscitated at . Although the resuscitation of C. jejuni biofilm and VBNC cells was not observed on smoked duck, microbial criteria of C. jejuni is needed in poultry and processed poultry products due to risk of its survival and low infectious dose.
C. jejuni;biofilm;viable but non-culturable (VBNC);smoked duck;
 Cited by
Korea Duck Association. 2013. Duck Consumption. (accessed Nov 2013).

Ministry of Food and Drug Safety. 2013. Foodborne Illness Statistics. (accessed Nov 2013).

Franco DA, Williams CE. 1994. Campylobacter jejuni. In Foodborne Disease Handbook. Hui YH, Gorham JR, Murrell KD, Cliver DO, eds. Marcel Dekker Inc., New York, NY, USA. p 71-96.

Skirrow MB. 1991. Epidemiology of Campylobacter enteritis. Int J Food Microbiol 12: 9-16. crossref(new window)

Stern N, Nachamkin I, Blaser M, Tompkins L. 1992. Reservoirs for Campylobacter jejuni and approaches for intervention in poultry. American Society for Microbiology, Washington, DC, USA. p 49-60.

Black RE, Levine MM, Clements ML, Hughes TP, Blaser MJ. 1988. Experimental Campylobacter jejuni infection in humans. J Infect Dis 157: 472-479. crossref(new window)

Moore JE, Corcoran D, Dooley JS, Fanning S, Lucey B, Matsuda M, McDowell DA, Megraud F, Millar BC, O'Mahony R, O'Riordan L, O'Rourke M, Rao JR, Rooney PJ, Sails A, Whyte P. 2005. Campylobacter. Vet Res 36: 351-382. crossref(new window)

Nachamkin I. 1999. Campylobacter and Arcobacter. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. 7th ed. ASM Press, Washington, DC, USA. p 716-726.

Ku HK, Park SR, Kim SK. 2008. Characterization of viable but nonculturable condition of Escherichia coli induced with copper. Korean J Microbiol Biotechnol 36: 209-214.

Bogosian G, Morris PJ, O'Neil JP. 1998. A mixed culture recovery method indicates that enteric bacteria do not enter the viable but nonculturable state. Appl Environ Microbiol 64: 1736-1742.

Day AP, Oliver JD. 2004. Changes in membrane fatty acid composition during entry of Vibrio vulnificus into the viable but nonculturable state. J Microbiol 42: 69-73.

Oliver JD. 2005. The viable but nonculturable state in bacteria. J Microbiol 43: 93-100.

Blaser MJ, Hardesty HL, Powers B, Wang WL. 1980. Survival of Campylobacter fetus subsp. jejuni in biological milieus. J Clin Microbiol 11: 309-313.

Moran AP, Upton ME. 1987. Factors affecting production of coccoid forms by Campylobacter jejuni on solid media during incubation. J Appl Bacteriol 62: 527-537. crossref(new window)

Rollins DM, Colwell RR. 1986. Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment. Appl Environ Microbiol 52: 531-538.

Jones DM, Sutcliffe EM, Curry A. 1991. Recovery of viable but non-culturable Campylobacter jejuni. J Gen Microbiol 137: 2477-2482. crossref(new window)

Stern NJ, Jones DM, Wesley IV, Rollins DM. 1994. Colonization of chicks by non-culturable Campylobacter spp. Lett Appl Microbiol 18: 333-336. crossref(new window)

Ziprin RL, Droleskey RE, Hume ME, Harvey RB. 2003. Failure of viable nonculturable Campylobacter jejuni to colonize the cecum of newly hatched leghorn chicks. Avian Dis 47: 753-758. crossref(new window)

Hengge-Aronis R. 1999. Interplay of global regulators and cell physiology in the general stress response of Escherichia coli. Curr Opin Microbiol 2: 148-152. crossref(new window)

Hall-Stoodley L, Costerton JW, Stoodley P. 2004. Bacterial biofilms: From the natural environment to infectious diseases. Nat Rev Microbiol 2: 95-108. crossref(new window)

Joshua GW, Guthrie-Irons C, Karlyshev AV, Wren BW. 2006. Biofilm formation in Campylobacter jejuni. Microbiology 152: 387-396. crossref(new window)

Reeser RJ, Medler RT, Billington SJ, Jost BH, Joens LA. 2007. Characterization of Campylobacter jejuni biofilms under defined growth conditions. Appl Environ Microbiol 73: 1908-1913. crossref(new window)

Sanders SQ, Boothe DH, Frank JF, Arnold JW. 2007. Culture and detection of Campylobacter jejuni within mixed microbial populations of biofilms on stainless steel. J Food Prot 70: 1379-1385.

Teh KH, Flint S, French N. 2010. Biofilm formation by Campylobacter jejuni in controlled mixed-microbial populations. Int J Food Microbiol 143: 118-124. crossref(new window)

Moe KK, Mimura J, Ohnishi T, Wake T, Yamazaki W, Nakai M, Misawa N. 2010. The mode of biofilm formation on smooth surfaces by Campylobacter jejuni. J Vet Med Sci 72: 411-416. crossref(new window)

Ica T, Caner V, Istanbullu O, Nguyen HD, Ahmed B, Call DR, Beyenal H. 2012. Characterization of mono-and mixedculture Campylobacter jejuni biofilms. Appl Environ Microbiol 78: 1033-1038. crossref(new window)

Park NY, Hong SH, Yoon KS. 2014. Effects of commercial marinade seasoning and a natural blend of cultured sugar and vinegar on Campylobacter jejuni and Salmonella Typhimurium and the texture of chicken breasts. Poult Sci 93: 719-727. crossref(new window)

Park NY, Ro EY, Jo HJ, Park KS, Yoon KS. 2014. Effect of packaging and temperature on survival kinetics of Campylobacter jejuni on precooked chicken breast. J Food Saf 34: 371-379. crossref(new window)

Rossoni EMM, Gaylarde CC. 2000. Comparison of sodium hypochlorite and peracetic acid as sanitising agents for stainless steel food processing surfaces using epifluorescence microscopy. Int J Food Microbiol 61: 81-85. crossref(new window)

Marques SC, Rezende JdGOS, Alves LAdF, Silva BC, Alves E, Abreu LRd, Piccoli RH. 2007. Formation of biofilms by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected chemical sanitizers. Braz J Microbiol 38: 538-543. crossref(new window)

Rodriguez A, McLandsborough LA. 2007. Evaluation of the transfer of Listeria monocytogenes from stainless steel and high-density polyethylene to Bologna and American cheese. J Food Prot 70: 600-606.

Kim SJ, Kim GH, Park JH, Park BG, Park MS, Oh DH. 2012. Analysis of transfer rate on Listeria monocytogenes contaminated pork meat during processing. J Fd Hyg Safety 27: 432-441. crossref(new window)

Wadhawan T, Maruska ZB, Siripattanakul S, Hill CB, Gupta A, Pruss BM, McEvoy JM, Khan E. 2011. A new method to determine initial viability of entrapped cells using fluorescent nucleic acid staining. Bioresour Technol 102: 1622-1627. crossref(new window)

Adzitey F, Huda N, Ali GR. 2012. Prevalence and antibiotic resistance of Campylobacter, Salmonella, and L. monocytogenes in ducks: A review. Foodborne Pathog Dis 9: 498-505. crossref(new window)

Flament A, Soubbotina A, Mainil J, Marlier D. 2012. Prevalence of Salmonella serotypes in male mule ducks in Belgium. Vet Rec 170: 311. crossref(new window)

Ku SK, Hwang SH, Lim SD, Lee KH, Kim YB. 2013. Nutritional characteristics and quality changes of duck by-products during frozen storage at-20$^{\circ}C$. Korean J Food Sci An 33: 109-118. crossref(new window)

Little CL, Richardson JF, Owen RJ, de Pinna E, Threlfall EJ. 2008. Prevalence, characterisation and antimicrobial resistance of Campylobacter and Salmonella in raw poultrymeat in the UK, 2003-2005. Int J Environ Health Res 18: 403-414. crossref(new window)

Uyttendaele M, De Troy P, Debevere J. 1999. Incidence of Listeria monocytogenes in different types of meat products on the Belgian retail market. Int J Food Microbiol 53: 75-80. crossref(new window)

Choi BG, Park JH, Kim HS, Jeon HR, Min JH, Yoon KS. 2015. Effect of slightly acidic electrolyzed water on the egg quality and the survival of S. enteritidis during storage. Abstract No P-J06 presented at the conference of the Korea Society of Foodservice Sanitation. Sookmyung Women's University, Seoul, Korea.

Michaelsen AR, Sebranek JG, Dickson JS. 2006. Effects of microbial inhibitors and modified atmosphere packaging on growth of Listeria monocytogenes and Salmonella enterica Typhimurium and on quality attributes of injected pork chops and sliced cured ham. J Food Prot 69: 2671-2680.

Ministry of Food and Drug Safety. 2016. Animal product of processing standards and component specification. &cmd=v (accessed Mar 2016).

Yoon KS, Burnette CN, Oscar TP. 2004. Development of predictive models for the survival of Campylobacter jejuni (ATCC 43051) on cooked chicken breast patties and in broth as a function of temperature. J Food Prot 67: 64-70.

Morey A, Singh M, McKee SR. 2012. Efficacy of manufacturer recommended microwave time against Listeria monocytogenes in ready-to-eat chicken products. Int J Poult Sci 11: 177-180. crossref(new window)

Midelet G, Carpentier B. 2002. Transfer of microorganisms, including Listeria monocytogenes, from various materials to beef. Appl Environ Microbiol 68: 4015-4024. crossref(new window)

Boulange-Petermann L, Baroux B, Bellon-Fontaine MN. 1993. The influence of metallic surface wettability on bacterial adhesion. J Adhesion Sci Technol 7: 221-230. crossref(new window)

Bryers JD. 1987. Biologically active surfaces: processes governing the formation and persistence of biofilms. Biotechnol Prog 3: 57-68. crossref(new window)

Stintzi A, Marlow D, Palyada K, Naikare H, Panciera R, Whitworth L, Clarke C. 2005. Use of genome-wide expression profiling and mutagenesis to study the intestinal lifestyle of Campylobacter jejuni. Infect Immun 73: 1797-1810. crossref(new window)

Perez-Rodriguez F, Valero A, Carrasco E, Garcia RM, Zurera G. 2008. Understanding and modelling bacterial transfer to foods: A review. Trends Food Sci Technol 19: 131-144. crossref(new window)